Proteomic analysis for RTK regulation by nitric oxide and its application to cancer therapy

• Project/Area Number: 23592016
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: SUGITA Hiroki 熊本大学, 医学部附属病院, 非常勤診療医師 (30398218)
• Co-Investigator(Kenkyū-buntansha): TAKAMORI Hiroshi 熊本大学, 医学部附属病院, 非常勤診療医師 (90363514)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: RTK / 一酸化窒素(NO) / IGF-iR / IRS-1 / PI3-K/Akt / Yeast two hybrid assay / 膵癌 / IGF-I / PI3-K/ Akt / NO / EGFR / MEK / MAPK / 増殖シグナル / 癌 / PI3-K

Research Abstract

To clarify the mechanisms of insulin-like growth factor(IGF) signaling regulated by nitric oxide, we performed Yeast Two Hybrid Assay which can search a protein binding to insulin receptor substrate( IRS)-1. Screening for cDNA library was performed. Eight proteins were selected as candidate of regulator of IRS-I. We found out that one of them bind to IRS-1 and regulated IGF signaling.

Research Products

• [Journal Article] Statins inhibit tumor progression via an enhancer of zeste homolog 2-mediated epigenetic alteration in colorectal cancer. (2014)
  • Author(s): S. Ishikawa, H. Hayashi, K. Kinoshita, M. Abe, H. Kuroki, R. Tokunaga, S. Tomiyasu, H. Tanaka, H. Sugita, T. Arita, Y. Yagi, M. Watanabe, M. Hirotaand H. Baba
  • Journal Title: Internationa Journal of Cancer Volume: Epub ahead of print Issue: 11 Pages: 2528-36
  • DOI: 10.1002/ijc.28672
  • Peer Reviewed
• [Journal Article] NO donor and MEK inhibitor synergistically inhibit proliferation and invasion of cancer cells (2012)
  • Author(s): Furuhashi S, Sugita H, Takamori H, Horino K, Nakahara O, Okabe H, Miyake K, Tanaka H, Beppu T, Baba H
  • Journal Title: Int J Oncol Volume: 40(3) Pages: 807-15
  • DOI: 10.3892/ijo.2011.1243
  • Peer Reviewed
• [Journal Article] Inducible nitric oxide synthase deficiency ameliorates skeletal muscle insulin resistance but does not alter unexpected lower blood glucose levels after burn injury in C57BL/6 mice. (2012)
  • Author(s): Michiko Sugita, Hiroki Sugita
  • Journal Title: Metabolism Volume: 61 Issue: 1 Pages: 127-136
  • DOI: 10.1016/j.metabol.2011.06.001
  • Peer Reviewed
• [Journal Article] The duration of SIRS before organ failure is a significant prognostic factor of sepsis (2012)
  • Author(s): Sugita H, Kinoshita Y, Baba H.
  • Journal Title: Int J Emerg Med. Volume: 5(1) Issue: 1 Pages: 44-44
  • DOI: 10.1186/1865-1380-5-44
  • Peer Reviewed
• [Journal Article] Distal pancreatectomy using a no-touch isolation technique (2012)
  • Author(s): Hirota M, Hashimoto D, Ishiko T, Satoh N, Takamori H, Chikamoto A, Tanaka H, Sugita H, Sand J, Nordback I, Baba H.
  • Journal Title: Scand J Surg Volume: 101(3) Pages: 156-9
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22968237
  • Peer Reviewed
• [Journal Article] Distal pancreatectomy using a no-touch isolation technique (2012)
  • Author(s): Masahiko Hirota, Hiroki Sugita
  • Journal Title: Scand J Surg Volume: 101 Issue: 3 Pages: 156-159
  • DOI: 10.1177/145749691210100303
  • Peer Reviewed
• [Presentation] 外科的手術が施行された残胃癌の予後についての検討 (2014)
  • Author(s): 杉田裕樹
  • Organizer: 第114回日本外科学会
  • Place of Presentation: 国立京都国際会館(発表確定)
  • Year and Date: 2014-04-03
• [Presentation] 外科的手術が施行された残胃癌の予後についての検討 (2014)
  • Author(s): 杉田 裕樹
  • Organizer: 第114回日本外科学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 絞扼性イレウスに対する腹腔鏡下イレウス解除術についての経験 (2013)
  • Author(s): 杉田裕樹
  • Organizer: 第26回日本内視鏡外科学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2013-11-28
• [Presentation] The role of nitric oxide in growth factor signaling of cancer cells (2011)
  • Author(s): Sugita H, Furuhashi S, Baba H.
  • Organizer: 2nd World DNA and Genome Day
  • Place of Presentation: Dalian, China
  • Year and Date: 2011-04-28
• [Book] Pancreatic Cancer, chapter : Nitric oxide regulates growth factor signaling in pancreatic cancer (2012)
  • Author(s): Sugita H,Furuhashi S, Baba H.
  • Publisher: Intech
• [Book] Pancreatic cancer, Molecular mechanism and targets (2012)
  • Author(s): Hiroki Sugita
  • Publisher: Intech
• [Book] Pancreatic Cancer, chapter: Nitric oxide regulates growth factor signaling in pancreatic cancer (2012)
  • Author(s): Sugita H, Furuhashi S, Baba H
  • Total Pages: 14
  • Publisher: Intech ISBN 978-953-307-815-1