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Proteomic analysis targeting stroma of pancreatic cancer

Research Project

Project/Area Number 23592018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionYokohama City University

Principal Investigator

ENDO Itaru  横浜市立大学, 医学(系)研究科(研究院), 教授 (60211091)

Co-Investigator(Kenkyū-buntansha) HIRANO Hisashi  横浜市立大学, 生命ナノシステム科学研究科, 教授 (00275075)
MORI Ryutaro  横浜市立大学, 附属病院, 助教 (90596412)
HIROSHIMA Yukihiko  横浜市立大学, 医学研究科, 客員研究員 (60718021)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords膵癌 / 癌微小環境 / 転移 / 浸潤 / ノックアウトマウス / 膵炎 / リン酸化 / 間質 / イメージング
Research Abstract

Pancreatic cancer has an aggressive malignancy. Recently, stroma in tumor is reported to be associated with cancer-cell proliferation, apoptosis, differentiation and invasion in several cancers. In this project, we comprehensively examined proteins expressing in the stroma of pancreatic tumors or of normal pancreas, and found that CRMP4 differentially expressed in pancreatic tumor stroma compared to normal pancreatic tissue. Of all the CRMPs, only crmp4 was differentially expressed in pancreatic cancer tissues. CRMP4 staining was highly correlated with poor differentiation and liver metastasis. Multivariate analyses suggested that venous invasion and CRMP4 overexpression were prognostic factors for survival. CRMP4 knockdown using siRNA reduced cellular invasion, but did not affect proliferation. Our results suggested that CRMP4 is significantly associated with poor prognosis by increasing pancreatic cancer cell invasiveness, and can be a novel therapeutic target for pancreatic cancer.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (18 results) Remarks (1 results)

  • [Journal Article] Hand-held high-resolution fluorescence imaging system for fluorescence-guided surgery of patient and cell-line pancreatic tumors growing orthotopically in nude mice.2014

    • Author(s)
      Hiroshima Y, Maawy A, Sato S, Murakami T, Uehara F, Miwa S, Yano S, Momiyama M, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM.
    • Journal Title

      J Surg Res.

      Volume: 187(2) Issue: 2 Pages: 510-7

    • DOI

      10.1016/j.jss.2013.11.1083

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of Salmonella typhimurium A1-R Versus Chemotherapy on a Pancreatic Cancer Patient-Derived Orthotopic Xenograft (PDOX).2014

    • Author(s)
      Hiroshima Y, Zhao M, Maawy A, Zhang Y, Katz MH, Fleming JB, Uehara F, Miwa S, Yano S, Momiyama M, Suetsugu A, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM.
    • Journal Title

      J Cell Biochem

      Volume: 115(7) Issue: 7 Pages: 1254-61

    • DOI

      10.1002/jcb.24769

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successful fluorescence-guided surgery on human colon cancer patient-derived orthotopic xenograft mouse models using a fluorophore-conjugated anti-CEA antibody and a portable imaging system.2014

    • Author(s)
      Hiroshima Y, Maawy A, Metildi CA, Zhang Y, Uehara F, Miwa S, Yano S, Sato S, Murakami T, Momiyama M, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM.
    • Journal Title

      J Laparoendosc Adv Surg Tech A.

      Volume: 24(4) Issue: 4 Pages: 241-7

    • DOI

      10.1089/lap.2013.0418

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hand-Held High-Resolution Fluorescence Imaging System for Fluorescence-Guided Surgery of Patient and Cell-Line Pancreatic Tumors Growing Orthotopically in Nude Mice2013

    • Author(s)
      Yukihiko Hiroshima, Ali Maawy, Sho Sato, Takashi Murakami, Fuminari Uehara, Shinji Miwa, Shuya Yano, Masashi Momiyama, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo and Robert M. Hoffman
    • Journal Title

      J Surg Res.

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Successful fluorescence-guided surgery on human colon cancer patient-derived orthotopic xenograft (PDOX) mouse models using a fluorophore-conjugated anti-CEA antibody and a portable imaging system2013

    • Author(s)
      Yukihiko Hiroshima, Ali Maawy, Cristina A. Metildi, Yong Zhang, Fuminari Uehara, Shinji Miwa, Shuya Yano, Sho Sato, Takashi Murakami, Masashi Momiyama, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo and Robert M. Hoffman
    • Journal Title

      J Laparoendosc Adv Surg Tech A.

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells.2013

    • Author(s)
      Hiroshima Y, Zhao M, Zhang Y, Maawy A, Hassanein MK, Uehara F, Miwa S, Yano S, Momiyama M, Suetsugu A, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM.
    • Journal Title

      Cell Cycle

      Volume: 1;12(17) Issue: 17 Pages: 2774-80

    • DOI

      10.4161/cc.25872

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Collapsin response mediator protein 4 expression is associated with liver metastasis and poor survival in pancreatic cancer2013

    • Author(s)
      Hiroshima Y, Nagashima Y et al
    • Journal Title

      Ann Surg Oncol

      Volume: 20 (Suppl 3) Issue: S3 Pages: 369-378

    • DOI

      10.1245/s10434-012-2491-3

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Collapsin Response Mediator Protein 4 Expression is Associated with Liver Metastasis and Poor Survival in Pancreatic Cancer.2012

    • Author(s)
      Hiroshima Yukihiko
    • Journal Title

      Annals of Surgical Oncology

      Volume: -

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Establishment of a patient-derived orthotopic xenograft (PDOX) model of patient cervical cancer2014

    • Author(s)
      Yukihiko Hiroshima1, Yong Zhang1, Ali Maawy2, Sho Sato3, Takashi Murakami3, Mako Yamamoto1, Fuminari Uehara1, Shinji Miwa1, Shuya Yano1, Masashi Momiyama3, Takashi Chishima3, Kuniya Tanaka3, Ali Maawy2, Itaru Endo3, Robert M. Hoffman1.
    • Organizer
      AACR
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] The advantages of patient-derived orthotopic xenograft (PDOX) of metastatic cervical cancer for individualized therapy compared to the PDX model2014

    • Author(s)
      Yukihiko Hiroshima1, Yong Zhang1, Ali Maawy2, Sho Sato3, Takashi Murakami3, Mako Yamamoto1, Fuminari Uehara1, Shinji Miwa1, Shuya Yano1, Masashi Momiyama3, Takashi Chishima3, Kuniya Tanaka3, Michael Bouvet2, Itaru Endo3, Robert M. Hoffman1.
    • Organizer
      AACR
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficacy of Salmonella typhimurium A1-R and anti-VEGF therapy on a patient-derived orthotopic xenograft (PDOX) pancreatic cancer model2014

    • Author(s)
      Yukihiko Hiroshima1, Ming Zhao1, Matthew H.G. Katz2, Jason B. Fleming2, Sho Sato3, Takashi Murakami3, Mako Yamamoto1, Fuminari Uehara1, Shinji Miwa1, Shuya Yano1, Masashi Momiyama3, Yong Zhang1, Ali Maawy4, Takashi Chishima3, Kuniya Tanaka3, Michael Bouvet4, Itaru Endo3, Robert M. Hoffman1.
    • Organizer
      AACR
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Zoledronic acid inhibits proliferation and metastasis of human pancreatic cancer in the patient-derived orthotopic xenograft (PDOX) model by targeting tumor-educated macrophages2014

    • Author(s)
      Yukihiko Hiroshima1, Mohamed K. Hassenein1, Rhiana Menen1, Matthew H.g. Katz2, Jason B. Fleming2, Sho Sato3, Takashi Murakami3, Mako Yamamoto1, Fuminari Uehara1, Shinji Miwa1, Shuya Yano1, Masashi Momiyama3, Ali Maawy4, Takashi Chishima3, Kuniya Tanaka3, Michael Bouvet4, Itaru Endo3, Robert M. Hoffman1.
    • Organizer
      AACR
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Efficacy of neoadjuvant chemotherapy in combination with fluorescence-guided surgery on a pancreatic cancer patient-derived orthotopic xenograft (PDOX)2014

    • Author(s)
      Yukihiko Hiroshima1, Ali Maawy1, Yong Zhang1, Sho Sato2, Takashi Murakami2, Mako Yamamoto1, Fuminari Uehara1, Shinji Miwa1, Shuya Yano1, Masashi Momiyama2, Takashi Chishima2, Kuniya Tanaka2, Michael Bouvet3, Itaru Endo2, Robert M. Hoffman1.
    • Organizer
      AACR
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] UVC irradiation in combination with fluorescence-guided surgery cures metastatic human pancreatic cancer in orthotopic mouse models2014

    • Author(s)
      Yukihiko Hiroshima1, Ali Maawy2, Sho Sato3, Takashi Murakami3, Mako Yamamoto1, Fuminari Uehara1, Shinji Miwa1, Shuya Yano1, Masashi Momiyama3, Takashi Chishima3, Kuniya Tanaka3, Michael Bouvet4, Itaru Endo3, Robert M. Hoffman1.
    • Organizer
      AACR
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Salmonella typhimurium A1-R effectively targets human-patient pancreatic tumorgrafts in nude mice2013

    • Author(s)
      Ming Zhao, Matthew H. G. Katz, Jason B. Fleming, Atsushi Suetsugu, Yong Zhang, Ali Maawy, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman, Yukihiko Hiroshima
    • Organizer
      ASCO annual meeting
    • Place of Presentation
      Chicago, IL
    • Related Report
      2013 Final Research Report
  • [Presentation] Salmonella typhimurium A1-R targets chemoresistant stem-like human pancreatic cancer cells2013

    • Author(s)
      Yukihiko Hiroshima, Ming Zhao, Yong Zhang, Ali Maawy, Mohamed K. Hassanein, Atsushi Suetsugu, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman
    • Organizer
      ASCO annual meeting
    • Place of Presentation
      Chicago, IL
    • Related Report
      2013 Final Research Report
  • [Presentation] Efficacy of Salmonella typhimurium A1-R on chemoresistant stem-like human pancreatic cancer cells2013

    • Author(s)
      Yukihiko Hiroshima, Ming Zhao, Yong Zhang, Ali Maawy, Mohamed Hassanein, Atsushi Suetsugu, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman
    • Organizer
      AACR annual meeting
    • Place of Presentation
      Washington, DC
    • Related Report
      2013 Final Research Report
  • [Presentation] Fluorescence-guided surgery for human-patient colon cancer tumorgrafts using a fluorophore-conjugated anti-CEA antibody2013

    • Author(s)
      Yukihiko Hiroshima, Ali Maawy, Yong Zhang, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman
    • Organizer
      AACR annual meeting
    • Place of Presentation
      Washington, DC
    • Related Report
      2013 Final Research Report
  • [Presentation] Tumor-educated macrophages promote proliferation of human pancreatic cancer cells in vitro and in vivo2013

    • Author(s)
      Yukihiko Hiroshima, Masashi Momiyama, Mohamed Hassanein, Ali Maawy, Rhiana Menen, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman
    • Organizer
      AACR annual meeting
    • Place of Presentation
      Washington, DC
    • Related Report
      2013 Final Research Report
  • [Presentation] Efficacy of Salmonella typhimurium A1-R treatment on human patient pancreatic tumorgrafts2013

    • Author(s)
      Yukihiko Hiroshima, Ming Zhao, Matthew H.G. Katz, Jason B. Fleming, Atsushi Suetsugu, Yong Zhang, Ali Maawy, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman
    • Organizer
      AACR annual meeting
    • Place of Presentation
      Washington, DC
    • Related Report
      2013 Final Research Report
  • [Presentation] Efficacy of Salmonella typhimurium A1-R on chemoresistant stem-like human pancreatic cancer cells2013

    • Author(s)
      Hiroshima Yukihiko
    • Organizer
      AACR annual meeting
    • Place of Presentation
      U.S.A., Washington, DC
    • Related Report
      2012 Research-status Report
  • [Presentation] Fluorescence-guided surgery for human-patient colon cancer tumorgrafts using a fluorophore-conjugated anti-CEA antibody2013

    • Author(s)
      Hiroshima Yukihiko
    • Organizer
      AACR annual meeting
    • Place of Presentation
      U.S.A., Washington, DC
    • Related Report
      2012 Research-status Report
  • [Presentation] Tumor-educated macrophages promote proliferation of human pancreatic cancer cells in vitro and in vivo2013

    • Author(s)
      Hiroshima Yukihiko
    • Organizer
      AACR annual meeting
    • Place of Presentation
      U.S.A., Washington, DC
    • Related Report
      2012 Research-status Report
  • [Presentation] Efficacy of Salmonella typhimurium A1-R treatment on human patient pancreatic tumorgrafts2013

    • Author(s)
      Hiroshima Yukihiko
    • Organizer
      AACR annual meeting
    • Place of Presentation
      U.S.A., Washington, DC
    • Related Report
      2012 Research-status Report
  • [Presentation] Collapsin response mediator protein 4 promotes tumor cell invasion and lethality of pancreatic cancer2012

    • Author(s)
      Yukihiko Hiroshima, Fumio Nakamura, Hiroshi Miyamoto, Ryutaro Mori, Koichi Taniguchi, Ryusei Matsuyama, Kazuhisa Takeda, Hirotoshi Akiyama, Kuniya Tanaka, Yasushi Ichikawa, Yoji Nagashima, Yoshio Goshima, Itaru Endo
    • Organizer
      AACR annual meeting
    • Place of Presentation
      Chicago, IL
    • Related Report
      2013 Final Research Report
  • [Presentation] Collapsin Response Mediator Protein 4 Promotes Tumor Cell Invasion and Lethality of Pancreatic Cancer2012

    • Author(s)
      廣島 幸彦
    • Organizer
      AACR Annual Meeting 2012
    • Place of Presentation
      アメリカ、シカゴ
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.ycusurg2.jp/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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