Development of artificial blood vessel using biocompatible nanofiber sheet as a scaffold to accerelate native tissue regeneration

• Project/Area Number: 23592026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Chiba University
• Principal Investigator: ISHIZAKA Toru 千葉大学, 医学部附属病院, 講師 (10372616)
• Co-Investigator(Kenkyū-buntansha): MATSUMIYA Goro 千葉大学, 医学研究院, 教授 (20314312) ; KOUNO Hiroki 千葉大学, 医学部附属病院, 助教 (40375803) ; ISHIDA Keiichi 千葉大学, 医学部附属病院, 助教 (40375671)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ナノファイバー / 人工血管 / 心臓血管外科学 / 生体組織工学

Research Abstract

<Objective> We sought to evaluate the usefulness of polyglutamic acid nanofiber sheet as a scaffold on the artificial arterial vessel. <Methods> Polyglutamic acid nanofiber sheet was attached to the woven polylactic acid vessel graft, which was transplanted under the skin in the thigh of the rat and was replaced of the abdominal aorta of the rabbit. <Results> Early infiltration of the native fibroblast cells was observed on the graft under the skin. Graft replacing the aorta invited the thrombo-occlusion of the abodominal aorta followed by paraplegia. <Conclusion> Polyglutamic acid nanofiber sheet showed high histocompatibility, but its thrombogenicity excluded the possibility of its use as an artificial blood vessel.