The Cardioprotective Effect Of Interleukin-11 in Cardiac Surgery

• Project/Area Number: 23592027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Chiba University
• Principal Investigator: KOUNO Hiroki 千葉大学, 医学部附属病院, 助教 (40375803)
• Co-Investigator(Kenkyū-buntansha): MATSUMIYA Goro 千葉大学, 大学院医学研究院, 教授 (20314312) ; ISHIZAKA Toru 千葉大学, 医学部附属病院, 講師 (10372616) ; ISHIDA Keiichi 千葉大学, 医学部附属病院, 助教 (40375671) ; FUJIO Yasushi 大阪大学, 薬学研究科, 教授 (20359839)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: IL-11 / 心臓血管外科 / 心筋保護効果

Research Abstract

<Objective> We sought to evaluate the cardioprotective effect of IL-11 during prolonged hypothermic ischemia and subsequent reperfusion using a rat heart donor model. <Methods> IL-11 was administered intravenously before harvesting the rat heart. The hearts were preserved in cold buffer for 6 hours, and then mounted onto a Langendorff perfusion apparatus for reperfusion using an oxygenated solution containing IL-11. Normal saline was used instead of IL-11 in the control group. Functional recovery of the reperfused heart was observed and measured, and apoptotic cells were counted using the TUNEL staining technique. <Results> Functional recovery was significant after 6 hours of cold ischemia in the IL-11 group. The number of apoptotic cells was also significantly smaller in the IL-11 group. <Conclusion> IL-11 significantly protected the heart in this rat ischemic model. This was associated with the prevention of apoptosis of the cells exposed to the ischemia-reperfusion injury.