|Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
<Objective> We sought to evaluate the cardioprotective effect of IL-11 during prolonged hypothermic ischemia and subsequent reperfusion using a rat heart donor model. <Methods> IL-11 was administered intravenously before harvesting the rat heart. The hearts were preserved in cold buffer for 6 hours, and then mounted onto a Langendorff perfusion apparatus for reperfusion using an oxygenated solution containing IL-11. Normal saline was used instead of IL-11 in the control group. Functional recovery of the reperfused heart was observed and measured, and apoptotic cells were counted using the TUNEL staining technique. <Results> Functional recovery was significant after 6 hours of cold ischemia in the IL-11 group. The number of apoptotic cells was also significantly smaller in the IL-11 group. <Conclusion> IL-11 significantly protected the heart in this rat ischemic model. This was associated with the prevention of apoptosis of the cells exposed to the ischemia-reperfusion injury.