Pre-clinical study on pulmonary perfusion preservation using modified ET-Kyoto solutions

• Project/Area Number: 23592056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: BANDO TORU 京都大学, 医学(系)研究科(研究院), 准教授 (20293954)
• Co-Investigator(Kenkyū-buntansha): DATE Hiroshi 京都大学, 大学院医学研究科, 教授 (60252962) ; AOYAMA Akihiro 京都大学, 大学院医学研究科, 助教 (60379047) ; SATO Masaaki 京都大学, 大学院医学研究科, 助教 (00623109) ; 黄 政龍 公益財団法人田附興風会, その他部局等, その他 (10271511) ; 藤永 卓司 京都大学, 医学(系)研究科(研究院), 助教 (00444456) ; 趙 向東 京都大学, 医学(系)研究科(研究院), 助教 (00444464)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 呼吸器外科 / 移植・再生医療 / トランスレーショナルリサーチ / 外科

Research Abstract

We evaluated the effects of continuous perfusion on pulmonary preservation using a rat and a dog ex vivo perfusion models aiming at clinical application of modified ET-K solution as a perfusate. Neither a potassium-reduced ET-K nor an ET-K without HES demonstrated sufficient pulmonary preservation for reliable clinical practice. In addition, supplementation with red blood cells to the perfusion solution also failed to show any significant improvements of the preservation effect. A further extensive modification is needed for the clinical application.

Research Products

• [Journal Article] Protective effect of surfactant inhalation against warm ischemic injury in an isolated rat lung ventilation model (2013)
  • Author(s): Ohsumi A, Chen F, Sakamoto J, Nakajima D, Kobayashi M, Bando T, Date H
  • Journal Title: PLoS ONE Volume: 8
  • NAID: 120005324900
  • Peer Reviewed
• [Journal Article] Protective effect of plasmin in marginal donor lungs in an ex vivo lung perfusion model (2013)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Nakajima D, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 32 Pages: 505-510
  • Peer Reviewed
• [Journal Article] Protective effect of plasmin in marginal donor lungs in an ex vivo lung perfusion model. (2013)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Nakajima D, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 32 Pages: 505-510
  • Peer Reviewed
• [Journal Article] Protective effect of surfactant inhalation against warm ischemic injury in an isolated rat lung ventilation model. (2013)
  • Author(s): Ohsumi A, Chen F, Sakamoto J, Nakajima D, Kobayashi M, Bando T, Date H
  • Journal Title: PLoS ONE Volume: 8
  • NAID: 120005324900
  • Peer Reviewed
• [Journal Article] Reconditioning of lungs donated after cardiac death using short-term hypothermic machine perfusion (2012)
  • Author(s): Nakajima D, Chen F, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: Transplantation Volume: 94 Pages: 999-1004
  • NAID: 120005398546
  • Peer Reviewed
• [Journal Article] Protective effect of pre-recovery surfactant inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Ohsumi A, Chen F, Sakamoto J, Nakajima D, Hijiya K, Motoyama H, Okita K, Kikuchi R, Yamada T, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31 Pages: 1136-1142
  • NAID: 120004874000
  • Peer Reviewed
• [Journal Article] Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Yamada T, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transpl Int Volume: 25 Pages: 1096-1105
  • Peer Reviewed
• [Journal Article] The effect of beta-2 adrenoreceptor agonost inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Zhao X, Sakai H, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31 Pages: 773-779
  • Peer Reviewed
• [Journal Article] Reconditioning of lungs donated after circulatory death with normothermic ex vivo lung perfusion (2012)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Ohsumi A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31 Pages: 187-193
  • NAID: 120003892139
  • Peer Reviewed
• [Journal Article] Reconditioning of lungs donated after circulatory death with normothermic ex vivo lung perfusion. (2012)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Ohsumi A, Bando T, Date H.
  • Journal Title: J Heart Lung Transplant Volume: 31 Pages: 187-93
  • NAID: 120003892139
  • Peer Reviewed
• [Journal Article] The effect of beta-2 adrenoreceptor agonist inhalation on lungs donated after cardiac death in a canine lung transplantation model. (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Zhao X, Sakai H, Bando T, Date H.
  • Journal Title: J Heart Lung Transplant Volume: 31 Pages: 773-9
  • Peer Reviewed
• [Journal Article] Thrapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model. (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Yamada T, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transpl int Volume: 25 Pages: 1096-105
  • Peer Reviewed
• [Journal Article] Protective effect of pre-recovery surfactant inhalation on lungs donated after cardiac death in a canine lung transplantation model. (2012)
  • Author(s): Ohsumi A, Chen F, Sakamoto J, Nakajima D, Hijiya K, Motoyama H, Okita K, Horita K, Kikuchi R, Yamada T, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31 Pages: 1136-42
  • NAID: 120004874000
  • Peer Reviewed
• [Journal Article] Reconditioning lungs donated after cardiac death using short-term hypothermic machine perfusion. (2012)
  • Author(s): Nakajima D, Chen F, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Journal Title: Transplantation Volume: 94 Pages: 999-1004
  • NAID: 120005398546
  • Peer Reviewed
• [Journal Article] Reconditioning of lungs donated after circulatory death with normothermic ex vivo lung perfusion. (2012)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Ohsumi A, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31 Pages: 187-193
  • NAID: 120003892139
  • Peer Reviewed
• [Journal Article] Hypothermic machine perfusion ameliorates ischemia-reperfusion injury in rat lungs from non-heart-beating donors (2011)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamot J, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92 Pages: 858-863
  • Peer Reviewed
• [Journal Article] Effects of preprocurement ventilation on lungs donated after cardiac death in a canine lung transplantation model (2011)
  • Author(s): Sakamoto J, Chen F, Yamada T, Nakajima D, Ohsumi A, Kikuchi R, Zhao X, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92 Pages: 864-870
  • NAID: 120004995874
  • Peer Reviewed
• [Journal Article] Comparison of ET-Kyoto solution and Perfadex as a preservation solution in a pig ex vivo lung perfusion model : impact of potassium level (2011)
  • Author(s): Okamoto T, Chen F, Zhang J, Choi H, Yamada T, Morikawa H, Nakayama E, Bando T, Date H
  • Journal Title: Transplant Proc Volume: 43 Pages: 1525-1528
  • Peer Reviewed
• [Journal Article] Comparison of ET-Kyoto solution and Perfadex as a preservation solution in a pig ex vivo lung perfusion model: Impact of potassium level. (2011)
  • Author(s): Okamoto T, Chen F, Zhang J, Choi H, Yamada T, Morikawa H, Nakayama E, Bando T, Date H
  • Journal Title: Transplant Proc Volume: 43 Pages: 1525-1528
  • Peer Reviewed
• [Journal Article] Effects of preprocurement ventilation on lungs donated after cardiac death in a canine lung transplantation model. (2011)
  • Author(s): Sakamoto J, Chen F, Yamada T, Nakajima D, Ohsumi A, Kikuchi R, Zhao X, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92 Pages: 864-870
  • NAID: 120004995874
  • Peer Reviewed
• [Journal Article] Hypothermic machine perfusion ameliorates ischemia-reperfusion injury in rat lungs from non-heart-beating donors. (2011)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Osumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92 Pages: 858-863
  • Peer Reviewed
• [Presentation] Protective effect of beta-2 adrenoreceptor agonist inhalation during ex vivo lung perfusion (2013)
  • Author(s): Kondo T, Chen F, Ohsumi A, Hijiya K, Motoyama H, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2013-09-04
• [Presentation] Protective effect of plasmin in marginal donor lungs in an ex vivo lung perfusion model (2013)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Kondo T, Nakajima D, Sakamoto J, Yamada T, Aoyama A, Sato M, Bando T, Date H
  • Organizer: The 33rd Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Montral, Quebec, Canada
  • Year and Date: 2013-04-26
• [Presentation] 我が国における肺移植の現状と課題 (2013)
  • Author(s): 板東徹、山田徹、佐藤雅昭、青山晃博、陳豊史、佐藤寿彦、園部誠、大政貢、松本いずみ、近藤健、本山秀樹、土屋恭子、大角明宏、伊達洋至
  • Organizer: 第113回日本外科学会定期学術集会、ワークショップ
  • Place of Presentation: 福岡
  • Year and Date: 2013-04-13
• [Presentation] Protective effect of plasmin in marginal donor lungs in an ex vivo lung perfusion model. (2013)
  • Author(s): Motoyama H, Chen F, Ohsumi A, Hijiya K, Okita K, Kondo T, Nakajima D, Sakamoto J, Yamada T, Aoyama A, Sato M, Bando T, Date H
  • Organizer: 33rd Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Montreal,CANADA
• [Presentation] Protective effect of beta-2 adrenoreceptor agonist inhalation during ex vivo lung perfusion (2013)
  • Author(s): Kondo T, Chen F, Ohsumi A, Hijiya K, Motoyama H, Yamada T, Sato M, Aoyama A, Bando T, Date H
  • Organizer: 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: Kyoto
• [Presentation] 我が国における肺移植の現状と課題 (2013)
  • Author(s): 板東 徹、山田 徹、佐藤雅昭、青山晃博、陳 豊史、佐藤寿彦、園部 誠、大政 貢、松本いずみ、近藤 健、本山秀樹、土屋恭子、大角明宏、伊達洋至
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] Ex vivo lung perfusion (EVLP)中のplasmin投与による心停止ドナー肺の機能改善効果 (2013)
  • Author(s): 本山秀樹、陳 豊史。大角明宏、土屋恭子、尾北賢治、近藤 健、阪本 仁、中島大輔、山田 徹、佐藤雅昭、青山晃博、佐藤寿彦、園部 誠、大政 貢、板東 徹、松本いずみ、伊達洋至
  • Organizer: 第30回日本呼吸器外科学会総会
  • Place of Presentation: 名古屋
• [Presentation] Ex Vivo lung perfusion施行中におけるβ-2受容体アゴニスト吸入の保護効果 (2013)
  • Author(s): 近藤 健、陳 豊史、大角明宏、土屋恭子、山田 徹、佐藤雅昭、青山晃博、園部 誠、大政 貢、板東 徹、伊達洋至
  • Organizer: 第49回日本移植学会総会
  • Place of Presentation: 京都
• [Presentation] Ex vivo lung perfusion (EVLP)中のplasmin投与による心停止ドナー肺の機能改善効果 (2013)
  • Author(s): 山秀樹、陳 豊史。大角明宏、土屋恭子、尾北賢治、近藤 健、阪本 仁、中島大輔、山田 徹、佐藤雅昭、青山晃博、佐藤寿彦、園部 誠、大政 貢、板東 徹、松本いずみ、伊達洋至
  • Organizer: 第29回日本肺および心肺移植研究会
  • Place of Presentation: 千葉市
• [Presentation] 新たな臓器保存液開発への挑戦、ET-Kyoto液 (2012)
  • Author(s): 板東徹
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川
  • Year and Date: 2012-10-25
  • Invited
• [Presentation] 肺移植における低温灌流保存法の確立 (2012)
  • Author(s): 中島大輔、陳豊史、尾北賢治、本山秀樹、土屋恭子、大角明宏、阪本仁、山田徹、佐藤雅昭、青山晃博、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田
  • Year and Date: 2012-05-17
• [Presentation] Hypothermic machine perfusion for lung preservation : technical development in a canine model (2012)
  • Author(s): Nakajima D, Chen F, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Sakai H, Bando T, Date H
  • Organizer: The 32nd Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2012-04-18
• [Presentation] The effect of beta-2 adrenoreceptor agonist inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Sakai H, Bando T, Date H
  • Organizer: The 32nd Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2012-04-18
• [Presentation] Reconditioning of lungs from non-heart-beating donors with normothermic ex vivo lung perfusion (2012)
  • Author(s): Nakajima D, Yamada T, Chen F, Sakamoto J, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Organizer: The 31st Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Diego, USA
  • Year and Date: 2012-04-16
• [Presentation] Hypothermic ex vivo perfusion prevents ischemia reperfusion injury in rat lungs from non-heart-beating donors (2012)
  • Author(s): Nakajima D, Yamada T, Chen F, Sakamoto J, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Organizer: The 31st Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Diego, USA
  • Year and Date: 2012-04-15
• [Presentation] Protective effect of ventilation before organ procurement on graft lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Sakamoto J, Yamada T, Chen F, Nakajima D, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Organizer: The 31st Annual Meeting of the International Heart and Lung Transplantation
  • Place of Presentation: San Diego, USA
  • Year and Date: 2012-04-14
• [Presentation] Therapeutic effect of surfactant inhalation on lungs donated after cardiac death in a canine transplantation model. (2012)
  • Author(s): Ohsumi A, Chen F, Sakamoto J, Nakajima D, Hijiya K, Motoyama H, Okita K, Horita K, Kikuchi R, Yamada T, Sakai H, Bando T, Date H
  • Organizer: 32nd Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Prague, Czech Republic
• [Presentation] Hypothermic machine perfusion for lung preservation: technical development in a canine model (2012)
  • Author(s): Nakajima D, ChenF, Okita K, Motoyama H, Hijiya K, Ohsumi A, Sakamoto J, Yamada T, Sato M, Aoyama A, Sakai H, Bando T, Date H
  • Organizer: 32nd Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Prague, Czech Republic
• [Presentation] The effect of beta-2 adrenoreceptor agonist inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Sakai H, Bando T, Date H
  • Organizer: 32nd Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Prague, Czech Republic
• [Presentation] ET-K液による臨床肺移植症例の検討 (2012)
  • Author(s): 板東 徹、佐藤雅昭、青山晃博、佐藤寿彦、陳 豊史、園部 誠、阪井宏彰、伊達洋至、松本いずみ
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田市
• [Presentation] イヌ心停止ドナー肺移植モデルにおけるサーファクタント吸入の肺保護効果 (2012)
  • Author(s): 大角明宏、陳 豊史、阪本 仁、中島大輔、土屋恭子、本山秀樹、尾北賢治、菊池柳太郎、堀田健太、山田 徹、阪井宏彰、板東 徹、伊達洋至
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田市
• [Presentation] 肺移植における低温灌流保存法の確立 (2012)
  • Author(s): 中島大輔、陳 豊史、尾北賢治、本山秀樹、土屋恭子、大角明宏、阪本 仁、山田 徹、佐藤雅昭、青山晃博、阪井宏彰、板東 徹、伊達洋至
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田市
• [Presentation] 新たな臓器保存液開発への挑戦、ET-Kyoto液． (2012)
  • Author(s): 板東 徹
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川市
  • Invited
• [Presentation] 常温肺体外灌流一normothermic ex-vo lung perfusion(EVLP)一による心停止ドナー肺の回復 (2011)
  • Author(s): 中島大輔、陳 豊史、山田 徹、阪本 仁、大角明宏、藤永卓司、庄司剛、阪井宏彰、板東 徹、伊達洋至
  • Organizer: 第28回日本呼吸器外科学会総会
  • Place of Presentation: 別府、ビーコンプラザ
• [Presentation] Beta2-adrenoreceptor agonist inhalation ameliorates ischemia reperfusion injury following lung transplantation from non-heart-beating donors in a canine model (2011)
  • Author(s): Jin Sakamoto, Fengshi Chen, Daisuke Nakajima, Tetsu Yamada, Akihiro Ohsumi, Xiangdong Zhao, Takuji Fujinaga, Tsuyoshi Shoji, Hiroaki Sakai, Toru Bando, Hiroshi Date
  • Organizer: The 15th European Society for Organ Transplantation Congress
  • Place of Presentation: Glasgow, UK
• [Presentation] Beta2-adrenoreceptor agonist inhalation ameliorates ischemia reperfusion injury following lung transplantation from non-heart-beating donors in a canine model (2011)
  • Author(s): Jin Sakamoto, Fengshi Chen, Daisuke Nakajima, Tetsu Yamada, Akihiro Ohsumi, Xiangdong Zhao, Takuji Fujinaga, Tsuyoshi Shoji, Hiroaki Sakai, Toru Bando, Hiroshi Date
  • Organizer: Congress of the Asian Society of Transplantation 2011
  • Place of Presentation: Seoul, Korea
• [Book] 臓器保存液の基礎.浅野武秀ら編集移植のための臓器摘出と保存 (2012)
  • Author(s): 板東徹
  • Publisher: 丸善出版
• [Book] 臓器保存液の基礎、浅野武秀ら編集 移植のための臓器摘出と保存、p7-10 (2012)
  • Author(s): 分担 板東 徹
  • Total Pages: 304
  • Publisher: 丸善出版
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/