Non-invasive assessment for acute rejection in lung transplantation.

• Project/Area Number: 23592057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: SAKAI HIROAKI 京都大学, 医学(系)研究科(研究院), その他 (50362489)
• Co-Investigator(Kenkyū-buntansha): DATE Hiroshi 京都大学, 医学研究科, 教授 (60252962) ; SHOJI Tuyoshi 公益財団法人田附興風会, 呼吸器外科, 研 究員 (80402840)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 強制オッシレーション法 / 急性肺拒絶評価 / 肺インピーダンス / tissue fraction / Tissue fraction (TF) / FOT / 急性肺拒絶反応 / lung structure / lung function / 強制オッシレーション / 急性肺拒絶評価法 / 呼吸インピーダンス

Research Abstract

After lung transplantation, early detection of acute rejection is important for timely and optimal treatment. We examined the relationship between lung mechanics and rejection grade in a rat acute rejection model using the forced oscillation technique (FOT), which provides non-invasive assessment of lung function. We assessed lung resistance and elastance (RL and EL) from implanted left lung of these animals. The rejection severity grade (A-grade) was quantified from histological images using tissue fraction (TF: tissue + cell infiltration area / total area). We found that TF, RL and EL worsened according to A-grade. Both Elow (at lowest frequency of EL) and Ehet (the absolute difference between Elow and maximum value of EL) showed correlation with the mean value of TF. Our results suggest that the dynamic elastance as well as its frequency dependence reflect A-grade. These indexes should prove useful for detecting and monitoring the progression disease in acute rejection.

Research Products

• [Journal Article] Redo living-donor lobar lung transplantation for bronchiolitis obliterans associated with antibody-mediated rejection. (2014)
  • Author(s): Chen F, Miyagawa-Hayashino A, Yurugi K, Chibana N, Yamada T, Sato M, Aoyama A, Takakura S,BandoT,Date H.
  • Journal Title: Transpl Int. Volume: 27
  • Peer Reviewed
• [Journal Article] Multi-frequency forced oscillation technique using impulse oscillations : Can it give mechanical information about the lung periphery? (2013)
  • Author(s): Hamakawa H, Sakai H, Takahashi A, bando T, Date H
  • Journal Title: Adv Exp Med Biol Volume: 765 Pages: 73-9
• [Journal Article] A prospective study analyzing one-year multidimensional outcomes in living lung transplant donors. (2013)
  • Author(s): Chen F, Oga T,Sakai H, Matsumoto I, Yamada T, Sato M, Aoyama A,BandoT, Mishima M, Chin K,Date H
  • Journal Title: Am J Transplant. Volume: 13 Pages: 3003-9
  • Peer Reviewed
• [Journal Article] Multi-frequency forced oscillation technique using impulse oscillations: can it give mechanical information about the lung periphery? (2013)
  • Author(s): Hamakawa H,Sakai H, Takahashi A, BandoT,Date H.
  • Journal Title: Adv Exp Med Biol. Volume: 765 Pages: 73-9
  • Peer Reviewed
• [Journal Article] Postoperative respiratory management in living donor lobar lung transplantation. (2013)
  • Author(s): Chen F, Chin K, Sato M, Aoyama A, Murase K, Azuma M, Bando T,Date H.
  • Journal Title: Clin Transplant. Volume: 27
  • Peer Reviewed
• [Journal Article] A NOD2 gene polymorphism is associated with the prevalence and severity of chronic obstructive pulmonary disease in a Japnese population (2012)
  • Author(s): Kinose D, Ogawa E, Hirota T, Ito I, Kubo M, Haruna A, Marumo S, Hoshino Y, Muro S, Hirai T, Sakai H, Date H, Tamari M, Miyoshi M
  • Journal Title: Respirology Volume: 17(1) Pages: 164-71
• [Journal Article] Current status of organ transplantation in Japan (2012)
  • Author(s): Egawa H, Tanabe K, Fukushima N, Date H, Sugitani A, Haga H
  • Journal Title: Am J Transplant Volume: 12(3) Pages: 523-30
• [Journal Article] Outcomes and pulmonary function in living lobar lung transplant donors (2012)
  • Author(s): Chen F, Fujinaga T, Shoji T, Sonobe M, Sato T, Sakai H, Bando T, Date H
  • Journal Title: Transpl Int Volume: 25(2) Pages: 153-7
• [Journal Article] Clinicopathological characteristics of surgically resected pulmonary pleomorphic carcinoma (2012)
  • Author(s): Chen F, Sonobe M, Sato T, Sakai H, Huang CL, Bando T, Date H
  • Journal Title: Eur J Cardiothorac Surg Volume: 41(5) Pages: 1037-42
• [Journal Article] Higher expression of EphA2 and ephrin-A1 is related to favorable clinicopathological features in pathological stage I non-small cell lung carcinoma (2012)
  • Author(s): Ishikawa M, Miyahara R, Sonobe M, Horiuchi M, Mennju T, Nakayama E, Kobayashi M, Kikuchi R, Kitamura J, Imamura N, Huang CL, Date H
  • Journal Title: Lung Cancer Volume: 76(3) Pages: 431-8
• [Journal Article] Date Reconditioning of lungs donated after circulatory death with normothermic ex vivo lung perfusion (2012)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Ohsume A, Bando T
  • Journal Title: J Heart Lung Transplant Volume: 31(2) Pages: 187-93
• [Journal Article] Date Current status of postoperative follow-up for lung cancer in Japan : questionnaire survey by the Setouchi Lung Cancer Study Group A0901 (2012)
  • Author(s): Sawada S, Suehisa H, Yamashita M, Nakata M, Okumura N, Okabe K, Nakamura H, Tada H, Toyooka S
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 60(2) Pages: 104-11
• [Journal Article] Pharmacokinetic study of weekly (day 1-5) low-dose S-1 in patients with non-small-cell lung cancer (2012)
  • Author(s): Shoji T, Sonobe M, Sakai H, Fujinaga T, Sato T, Chen F, Miyahara R, Bando T, Wada H, Date H
  • Journal Title: Rev Recent Clin Trials Volume: 7(2) Pages: 167-72
  • NAID: 120005285491
• [Journal Article] Short-term outcome in living donors for lung transplantation : the role of preoperative computer tomographic evaluations of fissures and vascular anatomy (2012)
  • Author(s): Chen F, Fujinaga T, Shoji T, Kubo T, Sonobe M, Sato M, Aoyama A, Sato T, Sakai H, Bando T, Date H
  • Journal Title: Transplant Int Volume: 25(7) Pages: 732-8
• [Journal Article] Induction chemoradiotherapy followed by surgical resection for clinical T3 and T4 locally advanced non-small cell lung cancer (2012)
  • Author(s): Shien k, Toyooka S, Kiura K, Matsuo K, Soh J, Yamane M, Oto T, Takemoto M, Date H, Miyoshi S
  • Journal Title: Ann Surg Oncol Volume: 19(8) Pages: 2685-92
• [Journal Article] Oxidative stress induced interleukin-32 mRNA expression in human bronchial epitherial cells (2012)
  • Author(s): Kudo M, Ogawa E, Kinose D, Haruna A, Takahashi T, Tahabe N, Marumo S, Hoshino Y, Hirai T, Sakai H, Muro S, Date H, Mishima M
  • Journal Title: Respir Res Volume: 13 Pages: 19-19
• [Journal Article] The effect ofβ-2 adrenoreceptor agonist inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Zhao X, Sakai H, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31(7) Pages: 773-9
• [Journal Article] Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Yamada T, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplant Int Volume: 25(10) Pages: 1096-105
• [Journal Article] Protective effect of pre-recovery surfactant inhalation on lungs donated after cardiac death in a canine lung transplantation model (2012)
  • Author(s): Osumi A, Chen F, Sakamoto J, Nakajima D, Hijiya K, Motoyama H, Okita K, Horita K, Kikuchi R, Yamada T, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: 31(10) Pages: 1136-42
  • NAID: 120004874000
• [Journal Article] Hyperfractionated irradiation with 3 cycles of induction chemotherapy in stage IIIA-N2 lung cancer (2012)
  • Author(s): Chen F, Okubo K, Sonobe M, Shibuya K, Matsuo Y, Kim YH, Yanagihara K, Bando T, Date H
  • Journal Title: World J Surg Volume: 36(12) Pages: 2858-64
• [Journal Article] Pulmonary function of individual lung lobes after complex living-donor lobar lung transplantation using inspiratory and expiratory three-dimensional computed tomographic volumetry (2012)
  • Author(s): Chen F, Fujinaga T, Bando T, Date H
  • Journal Title: Interact Cardiovasc Thorac Surg Volume: 15(6) Pages: 1077-9
• [Journal Article] Induction chemoradiotherapy is superior to induction chemotherapy for the survival of non-small-cell lung cancer patients with pathological mediastinal lymph node metastasis (2012)
  • Author(s): Toyooka S, Kiura K, Shien K, Katsui K, Hotta K, Kanazawa S, Date H, Miyoshi S
  • Journal Title: Interact Cardiovasc Thorac Surg Volume: 15(6) Pages: 954-60
• [Journal Article] The effect of β-2 adrenoreceptor agonist inhalation on lungs donated after cardiac death in a canine lung transplantation model. (2012)
  • Author(s): Sakamoto J, Chen F, Nakajima D, Yamada T, Ohsumi A, Zhao X, Sakai H, Bando T, Date H
  • Journal Title: J Heart Lung Transplant Volume: Jul;31(7) Pages: 773-9
  • NAID: 120004367785
  • Peer Reviewed
• [Journal Article] Clinicopathological characteristics of surgically resected pulmonary pleomorphic carcinoma. (2012)
  • Author(s): Chen F, Sonobe M, Sato T, Sakai H, Huang CL, Bando T, Date H
  • Journal Title: Eur J Cardiothorac Surg. Volume: May;41(5) Pages: 1037-42
  • Peer Reviewed
• [Journal Article] Outcomes and pulmonary function in living lobar lung transplant donors. (2012)
  • Author(s): Chen F, Fujinaga T, Shoji T, Sonobe M, Sato T, Sakai H, Bando T, Date H
  • Journal Title: Transpl Int. Volume: Feb;25(2) Pages: 153-7
  • Peer Reviewed
• [Journal Article] Therapeutic effect of surfactant inhalation during warm ischemia in an isolated rat lung perfusion model. (2012)
  • Author(s): Ohsumi A, Chen F, Nakajima D, Sakamoto J, Yamada T, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transpl Int. Volume: Oct;25(10) Pages: 1096-105
  • Peer Reviewed
• [Journal Article] Oxidative stress induced interleukin-32 mRNA expression in human bronchial epithelial cells. (2012)
  • Author(s): Kudo M, Ogawa E, Kinose D, Haruna A, Takahashi T, Tanabe N, Marumo S, Hoshino Y, Hirai T, Sakai H, Muro S, Date H, Mishima M
  • Journal Title: Respir Res. Volume: Mar 14
  • Peer Reviewed
• [Journal Article] Pharmacokinetic study of weekly (days 1-5) low-dose S-1 in patients with non-small-cell lung cancer. (2012)
  • Author(s): Shoji T, Sonobe M, Sakai H, Fujinaga T, Sato T, Chen F, Miyahara R, Bando T, Wada H, Date H
  • Journal Title: Rev Recent Clin Trials. Volume: May;7(2) Pages: 167-72
  • NAID: 120005285491
  • Peer Reviewed
• [Journal Article] A NOD2 gene polymorphism is associated with the prevalence and severity of chronic obstructive pulmonary disease in a Japanese population. Respirology. (2012)
  • Author(s): Kinose D, Ogawa E, Hirota T, Ito I, Kudo M, Haruna A, Marumo S, Hoshino Y, Muro S, Hirai T, Sakai H, Date H, Tamari M, Mishima M
  • Journal Title: Respirology Volume: Jan;17(1) Pages: 164-71
  • Peer Reviewed
• [Journal Article] Short-term outcome in living donors for lung transplantation: the role of preoperative computer tomographic evaluations of fissures and vascular anatomy. (2012)
  • Author(s): Chen F, Fujinaga T, Shoji T, Kubo T, Sonobe M, Sato M, Aoyama A, Sato T, Sakai H, Bando T, Date H
  • Journal Title: Transpl Int. Volume: Jul;25(7) Pages: 732-8
  • Peer Reviewed
• [Journal Article] Usefulness of endobronchial ultrasound in patients with previously treated thoracic malignancy. (2012)
  • Author(s): Chen F, Miyahara R, Sato T, Sonobe M, Sakai H, Bando T, Date H
  • Journal Title: Interact Cardiovasc Thorac Surg. Volume: Jan;14(1) Pages: 34-7
  • Peer Reviewed
• [Journal Article] Hypothermic machine perfusion ameliorates ischemia-reperfusion injury in rat lungs from non-heart-beating donors (2011)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Osumi A. Fujinaga T, Shoji T, Bando T, Date H
  • Journal Title: Transplantation Volume: 92(8) Pages: 858-63
• [Journal Article] Effect of preprocurement ventilation on lungs donated after cardiac death in a canine lung transplantation model (2011)
  • Author(s): Sakamoto J, Chen F, Yamada T, Nakajima D, Ohsumi A, Kikuchi R, Zhao X, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92(8) Pages: 864-70
  • NAID: 120004995874
• [Journal Article] Dynamic instability of central airways and peripheral airspace in rat lungs perfused with cold preservation solutions (2011)
  • Author(s): Hamakawa H, Sakai H, Takahashi A, Aoyama A, Zhang J, Chen F, Fujinaga T, Wada H, Date H, Bando T
  • Journal Title: Eur Surg Res Volume: 47(3) Pages: 159-67
• [Journal Article] Delayed chest closure assessed by transesophageal echocardiogram in single-lobe lung transplantation (2011)
  • Author(s): Chen F, Matsukawa S, Ishii H, Ikeda T, Shoji T, Fujinaga T, Bando T, Date H
  • Journal Title: Ann Thorac Surg Volume: 92(6) Pages: 2254-7
• [Journal Article] Clinical significance of BRAF gene mutations in patients with non-small cell lung cancer (2011)
  • Author(s): Kobayashi M, Sonobe M, Takahashi T, Yoshizawa A, Ishikawa M, Kikuchi R, Okubo K, Huang CL, Date H
  • Journal Title: Anticancer Res Volume: 31(12) Pages: 4619-23
• [Journal Article] Dynamic instability of central airways and peripheral airspace in rat lungs perfused with cold preservation solutions. (2011)
  • Author(s): Hamakawa H, Sakai H, Takahashi A, Aoyama A, Zhang J, Chen F, Fujinaga T, Wada H, Date H, Bando T
  • Journal Title: Eur Surg Res Volume: 47 Pages: 159-67
  • Peer Reviewed
• [Journal Article] A NOD2 gene polymorphism is associated with the prevalence and severity of chronic obstructive pulmonary disease in a Japanese population. (2011)
  • Author(s): Kinose D, Ogawa E, Hirota T, Ito I, Kudo M, Haruna A, Marumo S, Hoshino Y, Muro S, Hirai T, Sakai H, Date H, Tamari M, Mishima M
  • Journal Title: Respirology Volume: 10 Pages: 1440-1843
  • Peer Reviewed
• [Journal Article] Effect of preprocurement ventilation on lungs donated after cardiac death in a canine lung transplantation model. (2011)
  • Author(s): Sakamoto J, Chen F, Yamada T, Nakajima D, Ohsumi A, Kikuchi R, Zhao X, Fujinaga T, Shoji T, Sakai H, Bando T, Date H.
  • Journal Title: Transplantation Volume: 92(8) Pages: 864-70
  • NAID: 120004995874
  • Peer Reviewed
• [Journal Article] Hypothermic machine perfusion ameliorates ischemia-reperfusion injury in rat lungs from non-heart-beating donors. (2011)
  • Author(s): Nakajima D, Chen F, Yamada T, Sakamoto J, Osumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H
  • Journal Title: Transplantation Volume: 92(8) Pages: 858-63
  • Peer Reviewed
• [Journal Article] Living-donor lobar lung transplantation with sparing of bilateral native upper lobes: a novel strategy. (2011)
  • Author(s): Fujinaga T, Bando T, Nakajima D, Sakamoto J, Chen F, Shoji T, Sakai H, Ishii H, Miwa S, Date H
  • Journal Title: J Heart Lung Transplant Volume: 30(3) Pages: 351-3
  • Peer Reviewed
• [Presentation] Update in living-donor lobar lung transplantation (2012)
  • Author(s): Date H
  • Organizer: The Cleveland Clinic Pulmonary and Critial Care Fellow's Education Day
  • Place of Presentation: Cleveland
  • Year and Date: 2012-11-14
• [Presentation] Concepts and future perspectives of VATS (2012)
  • Author(s): Date H
  • Organizer: 2^<nd> ATEP
  • Place of Presentation: Shanghai
  • Year and Date: 2012-08-24
• [Presentation] Living-donor lobar lung transplantation (2012)
  • Author(s): Date H
  • Organizer: H.Lecture at Asan Medical Center
  • Place of Presentation: Seoul
  • Year and Date: 2012-06-17
• [Presentation] Living-donor lobar lung transplantation (2012)
  • Author(s): Date H
  • Organizer: Korean Society for Thoracic Oncology
  • Place of Presentation: Jeju
  • Year and Date: 2012-06-01
• [Presentation] 第30回呼吸器外科学会総会要望 (2012)
  • Author(s): 阪井宏彰、板東徹、陳豊史、藤永卓司、庄司剛、松本いづみ1、大藤剛宏、岡田克典、伊達洋至
  • Organizer: 肺移植適応患者の予後とQOL調査
  • Year and Date: 2012-05-18
• [Presentation] 第30回呼吸器外科学会総会 (2012)
  • Author(s): 阪井宏彰、板東徹、陳豊史、藤永卓司、庄司剛、松本いづみ、伊達洋至
  • Organizer: 生体肺移植ドナー患者のQOL前向き調査
  • Year and Date: 2012-05-17
• [Presentation] 肺移植適応患者の予後とQOLの調査 (2012)
  • Author(s): 阪井宏彰、板東徹、陳豊史、藤永卓司、庄司剛、松本いづみ1、大藤剛宏、岡田克典、伊達洋至
  • Organizer: 第29呼吸器外科学会示説50
  • Year and Date: 2012-05-13
• [Presentation] 生体肺移植ドナー患者における患者報告型アウトカムの調査 (2012)
  • Author(s): 阪井宏彰、板東徹、陳豊史、藤永卓司、庄司剛、松本いづみ、伊達洋至
  • Organizer: 第29回呼吸器外科学会示説50
  • Year and Date: 2012-05-01
• [Presentation] How to promote the quality and quantity of organ donors? (2012)
  • Author(s): Date H
  • Organizer: 71^<st> Annual Meeting of Taiwan Surgical Association
  • Place of Presentation: Taipei
  • Year and Date: 2012-03-17
• [Presentation] Transplantation from a non-heart-beating donor. An overview (2012)
  • Author(s): Date H
  • Organizer: 71^<st> Annual Meeting of Taiwan Surgical Association
  • Place of Presentation: Taipei
  • Year and Date: 2012-03-17
• [Presentation] Living-donor lung transplantation (2012)
  • Author(s): Date H
  • Organizer: The 20^<th> Annual Meeting of Asian Society for Cardiovascular and Thoracic Surgery
  • Place of Presentation: Bali
  • Year and Date: 2012-03-09
• [Presentation] Transplantation for non-transplant physicians : Postgraduate Symposium (2012.3.3 Leuven) Can I be a living lung donor? (2012)
  • Author(s): Date H :
  • Organizer: 13^<th> international symposium K. U. Leuven
  • Place of Presentation: Leuven
  • Year and Date: 2012-03-03
• [Presentation] Transplantation for non-transplant physicians : Workshop (2012.3.1-2 Leuven) Living-donor lobar lung transplantation (2012)
  • Author(s): Date H
  • Organizer: 13^<th> international symposium K. U. Leuven
  • Place of Presentation: Leuven
• [Presentation] 生体肺移植ドナー患者における患者報告型アウトカムの調査 (2012)
  • Author(s): 阪井 宏彰、板東 徹、陳 豊史、藤永 卓司、庄司 剛、松本 いづみ、伊達 洋至
  • Organizer: 第29回日本呼吸器外科学会
  • Place of Presentation: 秋田市
• [Presentation] 肺移植適応患者の予後とQOL の調査 (2012)
  • Author(s): 阪井 宏彰、板東 徹、陳 豊史、藤永 卓司、庄司 剛、松本 いづみ、大藤 剛宏、岡田 克典、伊達 洋至
  • Organizer: 第29回日本呼吸器外科学会
  • Place of Presentation: 秋田市
• [Presentation] Can non-sine curved oscillatory wave provide the mechanical information about the lung periphery? (2011)
  • Author(s): Hamakawa H, Sakai H, Takahashi A, Bando T , Date H
  • Organizer: ISOTT international conference
  • Place of Presentation: Georgetown U.S.A
• [Book] 肺移植、松原広己(編)肺高血圧症診療マニュアル (2012)
  • Author(s): 伊達洋至,伊藤浩
  • Publisher: 南江堂
• [Book] 肺移植、坂谷光則(編)びまん性肺疾患の臨床第4版 (2012)
  • Author(s): 伊達洋至,泉孝英
  • Publisher: 金芳堂
• [Book] Living-donor lobar lung transplantation for pulmonary arterial hypertension,eds. Textbook of Pulmonary Vascular Disease (2011)
  • Author(s): Yuan JXJ, Garcia JGN, Hales CA, Rich S, Archer SL, West JB
  • Total Pages: 1601
  • Publisher: Springer
• [Book] Living donor lobar lung transplantation,eds. Organ Transplantation. A Clinical Guide (2011)
  • Author(s): Date H : . In : Klein AA, Lewis CJ, Madsen JC
  • Total Pages: 128
  • Publisher: Cambridge University Press
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/