The role of Necl-5 in the invasive activity of lung adenocarcinoma
| Project/Area Number |
23592060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Kobe University |
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Principal Investigator |
MANIWA YOSHIMASA 神戸大学, 医学(系)研究科(研究院), 教授 (50362778)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Yoshitake 神戸大学, 大学院保健学研究科, 教授 (50189669)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 原発性肺癌 / 肺腺癌 / 細胞接着分子 / Necl5 / 浸潤能 / 上皮間葉移行 / 分子標的 / バイオマーカー / Necl-5 / 肺癌 / 癌宿主相互作用 / 癌浸潤 |
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| Research Abstract |
In this study,we used a bronchioloalveolar carcinoma (BAC) cell line and fibroblasts to assess the expression of Necl-5 in the development of cancer–stroma communication by using an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) systemthat enables visualization of cellmigration during invasion. The expression of Necl-5 was higher in BAC cells than in fibroblasts. This tendency didn't change even when the BAC cells were mixed with fibroblasts. To assess the role of Necl-5 in the invasive activity of the BAC cells, we knocked down its expression using RNA interference (RNAi). The invasion assay with DL-CGH revealed that inhibition of Necl-5 expression in the BAC cells was associated with suppressed invasiveness. Necl-5 expression in lung cancer cells is crucial for their invasiveness in the cancer–stromal interaction, suggesting that Necl-5 could be a favorable molecular target for the suppression of invasiveness in lung adenocarcinoma.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Slug increases sensitivity to tubulin-binding agents via the downregulation ofβIII andβIVa-tubulin in lung cancer cells2013
Author(s)
Tamura D, Arao T, Nagai T, Kaneda H, Aomatsu K, Fujita Y, Matsumoto K, De Velasco MA, Kato H, Hayashi H, Yoshida S, Kimura H, Maniwa Y, Nishio W, Sakai Y, Ohbayashi C, Kotani Y, Nishimura Y, Nishio K.
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Journal Title
Cancer Med
Volume: 2
Pages: 144-54
Related Report
Peer Reviewed
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[Journal Article] Slug increases sensitivity to tubulin-binding agents via the downregulation of βIII and βIVa-tubulin in lung cancer cells2013
Author(s)
Tamura D, Arao T, Nagai T, Kaneda H, Aomatsu K, Fujita Y, Matsumoto K, De Velasco MA, Kato H, Hayashi H, Yoshida S, Kimura H, Maniwa Y, Nishio W, Sakai Y, Ohbayashi C, Kotani Y, Nishimura Y, Nishio K
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Journal Title
Cancer Med
Volume: ２
Issue: 2
Pages: 144-154
DOI
Related Report
Peer Reviewed
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[Journal Article] MT1-MMP plays an important role in an invasive activity of malignant pleural mesothelioma cell2011
Author(s)
Doi T, Maniwa Y*, Tanaka Y, Tane S, Hashimoto S, Ohno Y, Nishio W, Nishimura Y, Ohbayashi C, Okita Y, Hayashi Y, Yoshimura M.
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Journal Title
Exp Mol Pathol
Volume: 90
Pages: 91-96
Related Report
Peer Reviewed
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