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Detection of aberrant expression of microRNAs and development of new diagnostics and treatment in lung cancer

Research Project

Project/Area Number 23592061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Thoracic surgery
Research InstitutionOkayama University

Principal Investigator

MIYOSHI Shinichiro  岡山大学, 医歯(薬)学総合研究科, 教授 (00190827)

Co-Investigator(Kenkyū-buntansha) TOYOOKA Shinichi  岡山大学, 大学院医歯薬学総合研究科, 教授 (30397880)
SOH Junichi  岡山大学, 岡山大学病院, 助教 (90559890)
TSUKUDA Kazunori  岡山大学, 岡山大学病院, 講師 (20346430)
ASANO Hiroaki  岡山大学, 岡山大学病院, 助教 (70534775)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords原発性肺癌 / マイクロRNA
Research Abstract

We detected that expression of miR-34b/c and miR-200c was inhibited in lung cancer. miR-34b/c was more frequently inhibited by methylation in small cell lung cancer. We detected that induction of miR-34b/c showed anti-tumor effect in malignant pleural mesothelioma in vivo. As for miR-200c, its expression was maintained in EGFR-mutant lung cancer cell lines and it was inhibited in EGFR-mutant lung cancer cell line with acquired resistance to EGFR-tyrosine kinase inhibitor (TKI). In addition, epithelial to mesenchymal transition (EMT) was observed in that TKI-resistant cell line. We also confirmed that induction of miR-200c to the TKI-resistant cell line resulted in the termination of EMT.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Preclinical evaluation of microRNA-34b/c deliverly for malignant pleural mesothelioma2014

    • Author(s)
      Ueno T, Toyooka S, Fukazawa T, Kubo T, Soh J, Asano H, Muraoka T, Tanaka N, Maki Y, Shien K, Furukawa M, Sakaguchi M, Yamamoto H, Tsukuda K, Miyoshi S
    • Journal Title

      Acta Med Okayama

      Volume: 68(1) Pages: 23-26

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/24553485

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preclinical evaluation of microRNA-34b/c delivery for malignant pleural mesothelioma.2014

    • Author(s)
      Ueno T, Toyooka S, Fukazawa T, Kubo T, Soh J, Asano H, Muraoka T, Tanaka N, Maki Y, Shien K, Furukawa M, Sakaguchi M, Yamamoto H, Tsukuda K, Miyoshi S.
    • Journal Title

      Acta Med Okayama

      Volume: 68 Pages: 23-26

    • NAID

      120005372648

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The degree of microRNA-34b/c methylation in serum-circulating DNA is associated with malignant pleural mesothelioma.2013

    • Author(s)
      Muraoka T, Soh J, Toyooka S, Aoe K, Fujimoto N, Hashida S, Maki Y, Tanaka N, Shien K, Furukawa M, Yamamoto H, Asano H, Tsukuda K, Kishimoto T, Otsuki T, Miyoshi S.
    • Journal Title

      Lung Cancer

      Volume: 82 Issue: 3 Pages: 485-490

    • DOI

      10.1016/j.lungcan.2013.09.017

    • NAID

      120005367626

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acquired resistance to EGFR inhibitors is associated with a manifestation of stem cell-like properties in cancer cells2013

    • Author(s)
      Shien K, Toyooka S, Yamamoto H, Soh J, Jida M, Thu KL, Hashida S, Maki Y, Ichihara E, Asano H, Tsukuda K, Takigawa N, Kiura K, Gazdar AF, Lam WL, Miyoshi S
    • Journal Title

      Cancer Res

      Volume: 73(10) Pages: 3051-61

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of microRNA-34 induces cell proliferation and invasion of human mesothelial cells2013

    • Author(s)
      Tanaka N, Toyooka S, Soh J, Tsukuda K, Shien K, Furukawa M, Muraoka T, Maki Y, Ueno T, Yamamoto H, Asano H, Otsuki T, Miyoshi S
    • Journal Title

      Oncol Rep

      Volume: 29(6) Issue: 6 Pages: 2169-74

    • DOI

      10.3892/or.2013.2351

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Acquired resistance to EGFR inhibitors is associated with a manifestation of stem cell-like properties in cancer cells.2013

    • Author(s)
      Shien K, Toyooka S, Yamamoto H, Soh J, Jida M, Thu KL, Hashida S, Maki Y, Ichihara E, Asano H, Tsukuda K, Takigawa N, Kiura K, Gazdar AF, Lam WL, Miyoshi S.
    • Journal Title

      Cancer Res.

      Volume: 73 Issue: 10 Pages: 3051-3061

    • DOI

      10.1158/0008-5472.can-12-4136

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequent methylation and oncogenic role of microRNA-34b/c in small-cell lung cancer2012

    • Author(s)
      Tanaka N, Toyooka S, Soh J, Kubo T, Yamamoto H, Maki Y, Muraoka T, Shien K, Furukawa M, Ueno T, Asano H, Tsukuda K, Aoe K, Miyoshi S
    • Journal Title

      Lung Cancer

      Volume: 76(1) Issue: 1 Pages: 32-8

    • DOI

      10.1016/j.lungcan.2011.10.002

    • NAID

      120005311982

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Acquisition of stem cell-like properties during acquired resistance to epidermal growth factor receptor inhibitors2013

    • Author(s)
      Shien K, Toyooka S, Yamamoto H, Soh J, Thu KL, Hashida S, Maki Y, Ichihara E, Asano H, Tsukuda K, Takigawa N, Kiura K, Gazdar AF, Lam WL, Miyoshi S
    • Organizer
      AACR Annual Meeting 2013
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2013-04-09
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Down-regulation of microRNA-34 induces cell proliferation and invasion of human mesothelial cells2012

    • Author(s)
      Tanaka N, Toyooka S, Soh J, Tsukuda K, Shien K, Furukawa M, Muraoka T, Maki Y, Ueno T, Asano H, Otsuki T, Miyoshi S
    • Organizer
      103rd AACR Annual Meeting
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2012-04-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 小細胞肺癌におけるmicroRNA-34b/cのメチル化とその役割2011

    • Author(s)
      田中則光、豊岡伸一、宗淳一、浅野博昭、佃和憲、三好新一郎
    • Organizer
      日本癌治療学会総会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] 小細胞肺癌におけるmicroRNA-34b/cのメチル化とその役割2011

    • Author(s)
      田中則光、豊岡伸一、宗淳一、浅野博昭、佃和憲、三好新一郎
    • Organizer
      日本癌治療学会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] Down-regulation of microRNA-34 induces cell proliferation and invasion of human mesothelial cells.

    • Author(s)
      Tanaka N, Toyooka S, Soh J, Tsukuda K, Shien K, Furukawa M, Muraoka T, Maki Y, Ueno T, Asano H, Otsuki T, Miyoshi S.
    • Organizer
      103rd AACR Annual Meeting
    • Place of Presentation
      Chicago, USA
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.nigeka-okayama-u.jp

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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