New treatment method of cerebral infarction with infiltrating cells
| Project/Area Number |
23592092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Ehime University |
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Principal Investigator |
KUMON YOSHIAKI 愛媛大学, 医学(系)研究科(研究院), 准教授 (80127894)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Hideaki 愛媛大学, 大学院医学系研究科, 講師 (30322275)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 脳血管障害学 / BINCs / MCP-1 / Fractalkine / CCR2 / CX3CR1 / cerebral infarction / cerebral ischemia / microglia |
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| Research Abstract |
BINCs (Brain Iba1+/NG2+ Cells) accumulate in the ischemic core (IC), and may play neuroprotectively. The chemokine-based mechanisms underlying the invasion of BINCs were investigated.
We found isolated BINCs expressed mRNA encoding CCR2 and CX3CR1 at high levels. Cultured astrocytes expressed mRNA encoding their ligands, MCP-1 and fractalkine (FK). Recombinant MCP-1 and/or FK, as well as astrocytes, induced the migration of BINCs in vitro. The mRNA for MCP-1, FK, CCR2, and CX3CR1 was expressed in the IC during the acute phase. Immunohistochemical studies revealed vascular endothelial cells and astrocytic endfeet expressed MCP-1 and FK. CCR2+/Iba1+ monocytes attached to the inside of vascular wall at 1 day post-ischemia (dpi), and there were CCR2+/CX3CR1+ macrophage-like cells in the parenchyma of the IC at 2 dpi, which may be progenitors for BINCs. These results suggest CCR2+ monocytes are first attracted to the IC by MCP-1+ endothelial cells and migrate toward FK+ astrocytic endfeet.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Expression of MCP-1 and Fractalkine on Endothelial cells and astrocytes may contribute to the invasion and migration of brain macrophages in ischemic rat brain lesions2013
Author(s)
Nari Tei, Junya Tanaka, Kana Sugimoto, Tasuku Nishihara, Ryutaro Nishioka, Hisaaki Takahashi, Hajime Yano, Shirabe Matsumoto, Shiro Ohue, Hideaki Watanabe, Yoshiaki Kumon, Takanori Ohnishi
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Journal Title
Journal of Neuroscience Research
Volume: 91
Pages: 681-693
Related Report
Peer Reviewed
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