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Protective effects of bone marrow-derived mononuclear cells in young mice on ischemic brain damage

Research Project

Project/Area Number 23592093
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cerebral neurosurgery
Research InstitutionFujita Health University (2012-2013)
Ehime University (2011)

Principal Investigator

HATA Ryuji  藤田保健衛生大学, 医学部, 教授 (90258153)

Co-Investigator(Kenkyū-buntansha) OHNISHI Takanori  愛媛大学, 大学院医学系研究科, 教授 (70233210)
KUMON Yoshiaki  愛媛大学, 大学院医学系研究科, 准教授 (80127894)
SAKANAKA Masahiro  愛媛大学, 大学院医学系研究科, 教授 (60170601)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordscerebral ischemia / aging / stem cell / transplantation / 脳虚血 / 骨髄単核球 / 再生医療 / 再生療法 / 骨髄幹細胞
Research Abstract

Circulating bone marrow-derived endothelial progenitor cells have been reported to be
originated from bone marrow-derived mononuclear cells (=BMCs) and implicated in homeostasis of the microvasculature. Decreased levels of circulating endothelial progenitor cells, associated with aging correlate with poor clinical outcomes in a range of cardiovascular diseases. Herein, we transplanted BMCs from young mice (=yBMCs) into aged mice before and after cerebral ischemia. Aged mice transplanted with yBMCs before ischemia reduced ischemic brain damage whereas animals transplanted with yBMCs after ischemia did not. Furthermore, aged mice transplanted with yBMCs depleted of CD34 positive cells before ischemia also reduced ischemic brain damage. Although further investigations must be required to elucidate the fundamental mechanism underlying senescence of the brain vasculature, our results shows the possibility that factors other than CD34 have protective effects on ischemic brain damage.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (22 results)

All 2014 2013 2012 2011 Other

All Journal Article (21 results) (of which Peer Reviewed: 20 results,  Open Access: 1 results) Remarks (1 results)

  • [Journal Article] Intravenous administration of bone marrow mononuclear cells alleviates hearing loss after transient cochlear ischemia through paracrine effects2014

    • Author(s)
      Takagi T, Yoshida T, Okada M, Hata R, Hato N, Gyo K and Hakuba N
    • Journal Title

      Neuroreport

      Volume: 16

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activated microglia in a rat stroke model express NG2 proteoglycan in peri-infarct tissue through the involvement of TGF-β12014

    • Author(s)
      Sugimoto K, Nishioka R, Ikeda A, Mise A, Takahashi H, Yano H, Kumon Y, Ohnishi T and Tanaka J.
    • Journal Title

      Glia

      Volume: 62 Pages: 185-98

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-tumor effects of various furocoumarins isolated from the roots, seeds and fruits of Angelica and Cnidium species under ultraviolet A irradiation2014

    • Author(s)
      Sumiyoshi M, Sakanaka M, Taniguchi M, Baba K and Kimura Y
    • Journal Title

      J Nat Med

      Volume: 68 Pages: 83-94

    • NAID

      40019919803

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of MCP-1 and fractalkine on endothelial cells and astrocytes may contribute to the invasion and migration of brain macrophages in ischemic rat brain lesions2013

    • Author(s)
      Tei N, Tanaka J, Sugimoto K, Nishihara T, Nishioka R, Takahashi H, Yano H, Matsumoto S, Ohue S, Watanabe H, Kumon Y and Ohnishi T
    • Journal Title

      J Neurosci Res

      Volume: 91 Pages: 681-93

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro and in vivo antiproliferative effect of a combination of ultraviolet-A and alkoxy furocoumarins isolated from Umbelliferae medicinal plants, in melanoma cells2013

    • Author(s)
      Kimura Y, Sumiyoshi M, Sakanaka M, Taniguchi M and Baba K
    • Journal Title

      Photochem Photobiol.

      Volume: 89 Pages: 1216-25

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modeling Alzheimer's Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβand Differential Drug Responsiveness2013

    • Author(s)
      Kondo T, Asai M, Tsukita K, Kutoku Y, Ohsawa Y, Sunada Y, Imamura K, Egawa N, Yahata N, Okita K, Takahashi K, Asaka I, Aoi T, Watanabe A, Watanabe K, Kadoya C, Nakano R, Watanabe D, Maruyama K, Hori O, Hibino S, Choshi T, Nakahata T, Hioki H, Kaneko T, Naitoh M, Yoshikawa K, Yamawaki S, Suzuki S, Hata R, Ueno S, Seki T, Kobayashi K, Toda T, Murakami K, Irie K, Klein WL, Mori H, Asada T, Takahashi R, Iwata N, Yamanaka S and Inoue H :
    • Journal Title

      Cell Stem Cell

      Volume: 12 Pages: 487-96

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A drug screening platform for Alzheimer's disease with intracellular Aβ oligomers using patient-specific iPSCs2013

    • Author(s)
      Kondo, T., (他24名), Murakami, K., Irie, K., Klein, W. L., Mori, H., Asada, T., Takahashi, R., Iwata, N., Yamanaka, S., Inoue, H
    • Journal Title

      Cell Stem Cell

      Volume: 12 Issue: 4 Pages: 487-496

    • DOI

      10.1016/j.stem.2013.01.009

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Modeling Alzheimer's Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug Responsiveness.2013

    • Author(s)
      Kondo T, Hata R, Ueno SI, Yamanaka S, Inoue H et al.
    • Journal Title

      Cell Stem Cell

      Volume: 12

    • NAID

      120006373212

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of ginsenoside Rb₁on skin changes2012

    • Author(s)
      Kimura Y, Sumiyoshi M and Sakanaka M
    • Journal Title

      J Biomed Biotechnol

    • Related Report
      2013 Final Research Report
  • [Journal Article] Accuracy of diffusion tensor magnetic resonance imaging-based tractography for surgery of gliomas near the pyramidal tract : a significant correlation between subcortical electrical stimulation and postoperative tractography2012

    • Author(s)
      Ohue S, Kohno S, Inoue A, Yamashita D, Harada H, Kumon Y, Kikuchi K, Miki H and Ohnishi T
    • Journal Title

      Neurosurgery

      Volume: 70 Pages: 283-93

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oct-3/4 promotes migration and invasion of glioblastoma cells2012

    • Author(s)
      Kobayashi K, Takahashi H, Inoue A, Harada H, Toshimori S, Kobayashi Y, Goto K, Sugimoto K, Yano H, Ohnishi T, and Tanaka J
    • Journal Title

      J Cell Biochem

      Volume: 113 Pages: 508-17

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preauricular transzygomatic anterior infratemporal fossa approach for tumors in or around infratemporal fossa lesions2012

    • Author(s)
      Ohue S, Fukushima T, Kumon Y, Ohnishi T, and Friedman AH
    • Journal Title

      Neurosurg Rev

      Volume: 35 Pages: 583-92

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prognosis of primary central nervous system lymphoma treated with radiotherapy alone2012

    • Author(s)
      Taguchi S, Hamamoto Y, Fujii T, Ochi T, Harada H, Ohnishi T, and Mochizuki T
    • Journal Title

      Jpn J Radiol

      Volume: 30 Pages: 806-10

    • NAID

      50008492394

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inflammatory pseudotumor in the lateral ventricle. Case report2012

    • Author(s)
      Ohue S, Kohno S, Matsui S, Kumon Y, and Ohnishi T
    • Journal Title

      Neurol Med Chir

      Volume: 52 Pages: 599-602

    • NAID

      10030694790

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Usefulness of endoscope-assisted microsurgery for removal of vestibular schwannomas2012

    • Author(s)
      Kumon Y, Kohno S, Ohue S, Watanabe H, Inoue A, Iwata S and Ohnishi T
    • Journal Title

      J Neurol Surg B Skull Base

      Volume: 73 Pages: 42-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted disruption of organic cation transporter 3 (Oct3) ameliorates ischemic brain damage through modulating histamine and regulatory T cells2012

    • Author(s)
      Zhu P, Hata R, Ogasawara M, Cao F, Kameda K, Yamauchi K, Schinkel AH, Maeyama K and Sakanaka M.
    • Journal Title

      J Cereb Blood Flow Metab

      Volume: 32 Pages: 1897-908

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted disruption of organic cation transporter 3 (Oct3) ameliorates ischemic brain damage through modulating histamine and regulatory T cells.2012

    • Author(s)
      Zhu P, Hata R, Ogasawara M, Cao F, Kameda K, Yamauchi K, Schinkel AH, Maeyama K, Sakanaka M
    • Journal Title

      J Cereb Blood Flow Metab

      Volume: 32 Pages: 1897-1908

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Subcutaneous injection containing IL-3 and GM-CSF ameliorates stab wound-induced brain injury in rats2011

    • Author(s)
      Nishihara T, Ochi M, Sugimoto K, Takahashi H, Yano H, Kumon Y, Ohnishi T and Tanaka J
    • Journal Title

      Exp Neurol

      Volume: 229 Pages: 507-16

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Risk of progression from mild memory impairment to clinically diagnosable Alzheimer disease in a Japanese community (from the Nakayama Study)2011

    • Author(s)
      Sonobe N, Hata R, Ishikawa T, Sonobe T, Matsumoto T, Toyota Y, Mori T, Fukunaga R, Komori K, Ueno S, Tanimukai S and Ikeda M
    • Journal Title

      Int Psychogeriatr

      Volume: 23 Pages: 772-779

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats2011

    • Author(s)
      Taguchi A, Zhu P, Cao F, Kikuchi-Taura A, Kasahara Y, Stern DM, Soma T, Matsuyama T and Hata R
    • Journal Title

      J Cereb Blood Flow Metab

      Volume: 31 Pages: 855-67

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats.2011

    • Author(s)
      Taguchi A, Zhu P, Cao F, Kikuchi-Taura A, Kasahara Y, Stern DM, Soma T, Matsuyama T and Hata R.
    • Journal Title

      J Cereb Blood Flow Metab.

      Volume: 31 Pages: 855-67

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Remarks] 講座情報

    • URL

      http://www.fujita-hu.ac.jp/~hnagashi/KDB/open/100011.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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