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C-type natriuretic peptide modulates permeability of the blood–brain barrier

Research Project

Project/Area Number 23592100
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKagoshima University

Principal Investigator

NAGAYAMA Tetsuya  鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (40336334)

Co-Investigator(Kenkyū-buntansha) MIYATA Atsurou  鹿児島大学, 医歯学総合研究科, 教授 (60183969)
KAMBE Yuki  鹿児島大学, 医歯学総合研究科, 助教 (60549913)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords血液脳関門 / C型ナトリウム利尿ペプチド / zonula occludens-1 / JunD / ドラッグデリバリーシステム / 脳卒中 / 血管内皮細胞
Research Abstract

C-type natriuretic peptide (CNP) is abundant in brain, but its effect on blood-brain barrier (BBB) permeability has not been clarified yet. Here, we examined this effect. Transendothelial electrical resistance (TEER) of in vitro BBB model, was significantly dose dependently decreased by CNP. CNP treatment reduced both the messenger RNA and protein expressions of zonula occludens-1 (ZO-1). The effects on TEER, mRNA, and protein expressions of ZO-1 were mimicked by cyclic GMP (cGMP) analog 8-bromo-cGMP and reversed by protein kinase G (PKG) inhibitor Rp-8-CPT-cGMPS, thus implying the role of PKG and cGMP signaling in BBB function. In vivo study of mouse brain by fluorimetric analysis with intravenous administration of sodium fluorescein also showed a significant increase in BBB permeability by CNP. These findings suggest that CNP modulates the BBB permeability by altering ZO-1.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] C-type natriuretic peptide modulates permeability of the blood-brain barrier2014

    • Author(s)
      M.Bohara, Y. Kambe, T. Nagayama, H, Tokimura, K. Arita, and A. Miyata
    • Journal Title

      J Cereb Blood Flow Metab

      Volume: vol.34 Pages: 589-596

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] C-type natriuretic peptide modulates permeability of the blood-brain barrier2014

    • Author(s)
      M. Bohara, Y. Kambe, T. Nagayama, H. Tokimura, K. Arita, and A. Miyata
    • Journal Title

      J Cereb Blood Flow Metab

      Volume: 34 Issue: 4 Pages: 589-596

    • DOI

      10.1038/jcbfm.2013.234

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] C型ナトリウム利尿ペプチドによる血液脳関門透過性の調節2013

    • Author(s)
      神戸悠輝、Manoj Bohara、栗原崇、永山哲也、有田和徳、宮田篤郎
    • Organizer
      第4回鹿児島神経科学研究会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2013-07-20
    • Related Report
      2013 Final Research Report
  • [Presentation] C型ナトリウム利尿ペプチドによる血液脳関門透過性の調節2013

    • Author(s)
      神戸悠輝、Manoj Bohara、栗原崇、永山哲也、有田和徳、宮田篤郎
    • Organizer
      第31回内分泌代謝セミナー
    • Place of Presentation
      ゆふいん山水館
    • Related Report
      2013 Final Research Report
  • [Presentation] C型ナトリウム利尿ペプチドによる血液脳関門透過性の調節2013

    • Author(s)
      神戸悠輝,Manoj Bohara,栗原崇,永山哲也,有田和徳,宮田篤郎
    • Organizer
      第31回 内分泌代謝学サマーセミナー
    • Place of Presentation
      ゆふいん山水館, 湯布院, 大分
    • Related Report
      2013 Annual Research Report
  • [Presentation] C型ナトリウム利尿ペプチドによる血液脳関門透過性の調節2013

    • Author(s)
      神戸悠輝,Manoj Bohara,栗原崇,永山哲也,有田和徳,宮田篤郎
    • Organizer
      第4回 鹿児島神経科学研究会
    • Place of Presentation
      鹿児島大学, 鹿児島, 鹿児島
    • Related Report
      2013 Annual Research Report
  • [Presentation] C-TYPE NATRIURETIC PEPTIDE MODULATES THE PERMEABILITY OF BLOOD-BRAIN BARRIER2013

    • Author(s)
      Manoj Bohara
    • Organizer
      第86回 日本薬理学会年会
    • Place of Presentation
      福岡,福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Role of free fatty acid receptor GPR40 in mouse models of inflammatory and neuropathic pain2012

    • Author(s)
      T. KURIHARA
    • Organizer
      Neuroscience 2012, SfN's 42nd annual meeting
    • Place of Presentation
      ニューオリンズ, Ernest N. Morial Convention
    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of C-type natriuretic peptide on blood-brain barrier function2011

    • Author(s)
      ボハラマノズ、宮田篤郎
    • Organizer
      第15回日本血管内分泌代謝学会学術総会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Related Report
      2013 Final Research Report
  • [Presentation] Effect of C-type natriuretic peptide on blood-brain barrier function2011

    • Author(s)
      ボハラマノズ,宮田篤郎
    • Organizer
      第15回 日本心血管内分泌代謝学会学術総会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府豊中市)
    • Related Report
      2011 Research-status Report
  • [Remarks] 生体情報薬理学, 研究業績

    • URL

      http://www.kufm.kagoshima-u.ac.jp/~pharmaco/achievement.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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