| Project/Area Number |
23592115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Niigata University |
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Principal Investigator |
USUI Hiroshi 新潟大学, 脳研究所, 非常勤講師 (20192510)
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| Co-Investigator(Kenkyū-buntansha) |
SAKIMURA Kenji 新潟大学, 脳研究所, 教授 (40162325)
WASHIYAMA Kazuo 新潟大学, 脳研究所, 准教授 (00183715)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脳腫瘍 / モデルマウス / 癌抑制遺伝子 / 細胞選択的機能破壊 / コンディシィショナルノックアウト |
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| Outline of Final Research Achievements |
In order to generate novel model mice for brain tumors, we produced Trp53 gene-knockout and Nf1 gene-conditional knockout mice, in which Trp53 gene was deleted in the whole body and Nf1 gene was destroyed specifically in the brain cells. For this purpose, Trp53-null/Nf1-flox mice were crossed with 3 types of Cre-knock in mice where Cre recombinase were expressed in the brain-specific manner (Nestin-Cre, GFAP-Cre, and NG2-Cre mice). Most of the resulting mice died by 9 months-old and expressed brain tumors in high frequency. Among them, when Trp53-null/Nf1-flox mice were crossed with the NG2-Cre mice in which Cre recombinase was expressed in NG2 glial cells (oligodendroglia progenitor cells), 89% (32/36) of mice exhibited brain tumors, most of which were oligodendrogliomas or anaplastic oligodendrogliomas. Our results showed that the model mice of brain tumors were efficiently produced by the conditional knockout method.
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