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Mechanism of glioma invasion and its inhibition

Research Project

Project/Area Number 23592118
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cerebral neurosurgery
Research InstitutionUniversity of Fukui

Principal Investigator

KITAI Ryuhei  福井大学, 医学部, 准教授 (80251990)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsGlioma / Brain tumor / Invasion / Detection / グリオーマ / 浸潤先端 / 5ALA / サイトカイン / 脳腫瘍
Research Abstract

The motility of cultured glioma cells were estimated by video monitor. The glioma cell line A172 was the most moving cells in other cells such as U87, T98G and primary glioma cell culture. The cells stimulated by low Oxygen chamber and low pH showed initial cell halt and following high motility. The inhibition of these motility was blocked by interferon beta. As for the detection of invasion front, recently 5ALA photodynamic diagnosis was introduced in glioma surgery. Using cultured cells, the fluorescent intensity and cell density were determined. The detection limit by the operator's naked eyes was reported.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2014 2013 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (9 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Tumor cell density required for macroscopic observation of 5-ALA-induced fluorescence of protoporphyrin-IX: Measurement in cultured glioma cells and clinical cases2014

    • Author(s)
      Kitai R, Takeuchi H, N Miyoshi, et al
    • Journal Title

      No Shinkei Geka

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Large Intramedullary Neurofibroma in the Thoracic Spinal Cord: Case Report2014

    • Author(s)
      Arishima H, Kitai R, Kodera T, Yamada S, Kikuta KI
    • Journal Title

      Neurol Med Chir

    • Related Report
      2013 Final Research Report
  • [Journal Article] The adaptation of a neuroendoscopic sheath to a Leksell stereotactic frame to make it suitable for approaching small ventricles: a technical note2014

    • Author(s)
      Kitai R, Nakano K, Hashimoto N, Arishima H, Yamauchi T, Higashino Y, Takeuchi H, Kikuta K. J Neurol Surg A
    • Journal Title

      J Neurol Surg A Cent Eur Neurosurg

      Volume: 75 Pages: 151-4

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 5-ALA誘導Protoporphyrin-IXの蛍光を視認できる腫瘍細胞密度の検討  -培養グリオーマ細胞と臨床例での測定2014

    • Author(s)
      北井隆平、竹内浩明、三好憲雄*、Bibin Andriana* 根石拡行、橋本智哉、菊田健一郎
    • Journal Title

      脳神経外科

      Volume: 6

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frontal meningioma incidentally detected in the routine preoperative assessment of tongue cancer: a case report and literature review2013

    • Author(s)
      Sano K, Kitai R, Yoshimura H, Ohba S
    • Journal Title

      J Oral Maxillofac Surg.

      Volume: 2195

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intraoperative computed tomography for cervicomedullary decompression of foramen magnum stenosis in achondroplasia: two case reports2013

    • Author(s)
      Arishima H, Tsunetoshi K, Kodera T, Kitai R, Takeuchi H, Kikuta K
    • Journal Title

      Neurol Med Chir

      Volume: 53 Pages: 902-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Coincident choroid plexus carcinoma and adrenocortical tumor in an infant2013

    • Author(s)
      Yoshida K, Sato K, Kitai R, Hashimoto N, Kubota T, Kikuta K
    • Journal Title

      Brain Tumor Pathol

      Volume: 30(2) Pages: 104-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Measurement and cellular sources of the soluble interleukin-2 receptor in primary central nervous system lymphoma2013

    • Author(s)
      Kitai R, Sasaki H, Matsuda K, Tsunetoshi K, Yamauchi T, Neishi H, Matsumura K, Tsunoda A, Takeuchi H, Sato K, Kikuta K
    • Journal Title

      Brain Tumor Pathol

      Volume: (1) Pages: 34-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathological features in the recurrence of oligodendroglioma and diffuse astrocytoma2012

    • Author(s)
      Takeuchi H, Hosoda T, Kitai R, Kodera T, Arishima H, Tsunetoshi K, Neishi H, Yamauchi T, Sato K, Imamura Y, Itoh H, Kubota T, Kikuta K
    • Journal Title

      Brain Tumor Pathol

      Pages: 140-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glioblastoma with oligodendroglial components2012

    • Author(s)
      Takeuchi H, Hosoda T, Kitai R, Kodera T, Arishima H, Tsunetoshi K, Neishi H, Yamauchi T, Sato K, Imamura Y, Itoh H, Kubota T, Kikuta K
    • Journal Title

      Brain Tumor Pathol

      Volume: (3) Pages: 154-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lymphomatosis cerebri : clinical characteristics, neuroimaging, and pathological findings2012

    • Author(s)
      Kitai R, Hashimoto N, Yamate K, Ikawa M, Yoneda M, Nakajima T, Arishima H, Takeuchi H, Sato K, Kikuta K
    • Journal Title

      Brain Tumor Pathol.

      Volume: (1) Pages: 47-53

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Measurement and cellular sources of the soluble interleukin-2 receptor in primary central nervous system lymphoma.2012

    • Author(s)
      Kitai R, Sasaki H, Matsuda K, Tsunetoshi K, Yamauchi T, Neishi H, Matsumura K, Tsunoda A, Takeuchi H, Sato K, Kikuta KI.
    • Journal Title

      Brain Tumor Pathol.

      Volume: in press Issue: 1 Pages: 34-39

    • DOI

      10.1007/s10014-012-0093-1

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Usefulness of intraoperative computed tomography in surgery for low-grade gliomas : a comparative study between two series without and with intraoperative computed tomography2011

    • Author(s)
      Hosoda T, Takeuchi H, Hashimoto N, Kitai R, Arishima H, Kodera T, Higashino Y, Sato K, Kikuta K
    • Journal Title

      Neurol Med Chir

      Volume: 51(7) Pages: 490-5

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] High Grade Glioma術中5ALA蛍光診断における限界蛍光強度の定量化細胞培養と臨床検体の対比2012

    • Author(s)
      北井隆平
    • Organizer
      日本脳神経外科学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2012-10-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 病理組織からみた膠芽腫の浸潤境界および再発GBMにおける5ALA蛍光手術の限界2012

    • Author(s)
      北井隆平
    • Organizer
      脳腫瘍病理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-05-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 病理組織から見た膠芽腫浸潤境界および再発GBMにおける5-ALA蛍光手術の限界2012

    • Author(s)
      北井隆平、竹内浩明、根石拡行、常俊顕三、山内貴寛、三好憲雄、菊田健一郎
    • Organizer
      第30回日本脳腫瘍病理学会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] 5ALA陽性と視認できる悪性神経膠腫、腫瘍細胞密度の検討-培養細胞での実験と臨床例との対比2012

    • Author(s)
      北井隆平、多田愛弓、山田真輔、荒井大志、根石拡行、常俊顕三、磯﨑 誠、有島英孝、小寺俊昭、竹内浩明、菊田健一郎、三好憲雄
    • Organizer
      第8回日本脳神経外科光線力学学会
    • Place of Presentation
      つくば
    • Related Report
      2012 Research-status Report
  • [Presentation] Brain Wave Electrode Using Multi-Wall Carbon Nanotube-dispersed Thin Film.2012

    • Author(s)
      K.Awara, R.Kitai, K.Kikuta, A.Kawamoto.
    • Organizer
      IUMRS-ICEM2012
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 脳出血で発症したgliosarcoma2症例の臨床病理組織学的検討2012

    • Author(s)
      荒井大志、常俊顕三、根石拡行、磯﨑 誠、有島英孝、小寺俊昭、北井隆平、竹内浩明、菊田健一郎
    • Organizer
      第83回日本脳神経外科学会中部支部学術集会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] 術中画像(i-CT)を用いたグリオーマ手術の検証2012

    • Author(s)
      竹内浩明、根石拡行、常俊顕三、磯﨑 誠、有島英孝、小寺俊昭、北井隆平、佐藤一史、菊田健一郎
    • Organizer
      第17回日本脳腫瘍の外科学会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 術中イメージ(i-CT)を用いたlow grade gliomaの手術治療戦略2012

    • Author(s)
      竹内浩明、常俊顕三、根石拡行、磯﨑 誠、有島英孝、小寺俊昭、荒井大志、山田真輔、多田愛弓、北井隆平、佐藤一史、菊田健一郎
    • Organizer
      第71回日本脳神経外科学会総会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] high grade glioma術中5ALA蛍光診断における限界蛍光強度の定量化、細胞培養と臨床検体の対比2012

    • Author(s)
      北井隆平、多田愛弓、山田真輔、荒井大志、根石拡行、磯﨑 誠、常俊顕三、有島英孝、小寺俊昭、竹内浩明、菊田健一郎
    • Organizer
      第71回日本脳神経外科学会総会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Book] 病気の分子形態学Central neurocytoma2011

    • Publisher
      学際企画
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.med.u-fukui.ac.jp/NOUGE/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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