| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
In the present study, we tested tumor tropic activity of mouse iPSCs using in vitro Matrigel Invasion assay and in vivo mouse intracranial tumor model. Mouse iPS cells showed a significant tropism to the conditioned media prepared from glioma cell lines and this tropism to the glioma conditioned media was partially blocked by the neutralizing antibodies for four major tumor-associated growth factors. The receptors for those growth factors, measured by reverse transcriptase polymerase chain reaction, were highly up-regulated in the mouse iPS cells compared to the mouse fibroblasts. Mouse iPSCs labeled with 5-bromo-2-deoxyuridine were intracranially implanted in the contralateral hemisphere to the GL261 gliomacell implantation in the allogeneic C57BL/6 mouse. Active migration of iPSCs was observed 7 days after implantation. These findings demonstrated that iPSCs had potent glioma tropism and could be candidates as vehicles in stem cell-based glioma therapy.
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