Development of boron-conjugate targeting adenovirus vector for novel boron neutron capture therapy

• Project/Area Number: 23592126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Hiroshima University
• Principal Investigator: HAMA Seiji 広島大学, 医歯薬保健学研究院(医), 研究員 (40397980)
• Co-Investigator(Kenkyū-buntansha): KURISU Kaoru 広島大学, 医歯薬保健学研究科, 教授 (70201473) ; HOSHI Masaharu 広島大学, 平和科学研究センター, 名誉教授 (50099090) ; NISHIMOTO Takeshi 広島大学, 病院, 医科診療医 (40450580)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: ホウ素中性子捕捉療法 / 悪性グリオーマ / アデノウイルスベクター / 放射線治療 / ホウ素化合物 / 電子顕微鏡 / 中性子捕捉療法 / ホウ素 / 放射線療法 / ICP / 脳腫瘍学

Outline of Final Research Achievements

We have conducted the research using adenovirus vector in order to enhance the effect of Boron neutron capture therapy (BNCT) for malignant brain tumor patients. At first, the gold colloid were bound on the surface of adenovirus vector using the binding reaction between thiol group (adenovirus vector surface) - maleimide compound (gold colloid). Then we confirmed that the glima cells were infected with gold colloid binding adenovirus vector using electronic microscope (EM). While ready-made boron compound could not be induced sufficient doses of intra-cellular boron using the adenovirus vector infection. Thus we are now proceeding with the synthesis of novel boron compound in order to improve binding efficiency on the adenovirus surface, lead to construct novel drug delivery system for BNCT.

Research Products

• [Journal Article] p16 Gene Transfer Induces Centrosome Amplification and Abnormal Nucleation Associated with Survivin Downregulation in Glioma Cells (2015)
  • Author(s): Takayasu T, Hama S, Yamasaki F, Saito T, Watanabe Y, Nosaka R, Sugiyama K, Kurisu K.
  • Journal Title: Pathobiology Volume: 82 Pages: 1-8
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 悪性グリオーマへのp16導入によるsurvivin発現低下を伴った核異常と中心体過剰複製 (2014)
  • Author(s): 高安武志、濱聖司、山崎文之、野坂亮、杉山一彦、栗栖薫
  • Organizer: 第32回日本脳腫瘍学会学術集会
  • Place of Presentation: シェラトン・グランデ・トーキョーベイ・ホテル
  • Year and Date: 2014-11-30 – 2014-12-02
• [Presentation] 脈絡乳頭癌の1例 (2014)
  • Author(s): 高安武志、山崎文之、野坂亮、杉山一彦、栗栖薫
  • Organizer: 第32回日本脳腫瘍病理学会
  • Place of Presentation: あわぎんホール（徳島県郷土文化会館）
  • Year and Date: 2014-05-23 – 2014-05-24
• [Presentation] 悪性グリオーマにおけるp16遺伝子導入による核異常と中心体過剰複製との関連性の検討
  • Author(s): 高安武志、濱聖司、栗栖薫他
  • Organizer: 日本脳神経外科学会第71回学術総会
  • Place of Presentation: 大阪国際会議場
• [Presentation] 悪性グリオーマにおけるp16遺伝子導入による核異常と中心体過剰複製との関連性の検討
  • Author(s): 高安武志、濱聖司、栗栖薫他
  • Organizer: 第30回日本脳腫瘍学会学術集会
  • Place of Presentation: グランドプリンスホテル広島
• [Presentation] Plastic ependymoma様の伸展を示した後頭蓋窩atypical teratoid/rhabdoid tumorの１例
  • Author(s): 高安武志、栗栖薫他
  • Organizer: 第30回日本脳腫瘍病理学会
  • Place of Presentation: 名古屋国際会議場