| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Accumulating evidence shows that the expression level of Oct-3/4, a self-renewal regulator in stem cells, is positively correlated with the progression of various solid tumors. However, little is known regarding the influence of Oct-3/4 in the tumor angiogenesis of glioblastoma. In this study, we subcutaneously transplanted U251/EGFP-Oct-3/4 cells into nude mice to evaluate the roles of Oct-3/4 in the tumor angiogenesis. Both tumor size and the number of large vessels growing in the tumor were markedly increased. In an in vitro model of angiogenesis, conditioned media from U251/EGFP-Oct-3/4 cells significantly accelerated capillary-like tube formation. In U251/EGFP-Oct-3/4 cells, VEGF mRNA was upregulated under the control of HIF1a, and enhanced protein expression and nuclear translocation of HIF1a were observed. Our results demonstrate that Oct-3/4-expressing glioma cells have the ability to adapt to low-oxygen environments of tumor by promoting tumor angiogenesis.
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