| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Pre-mRNA splicing of gene encoding pyruvate kinase M (PKM), a glycolytic enzyme, was dys-regulated, so that expression of PKM isoforms switches from normal- to tumor-type in human glioma. This aberrant splicing was associated with increased levels of a splicing modulator. Experiments using cell-lines showed that suppression of the splicing regulator results in partial restoration of expression of a normal-type PKM isoform. Additionally, we developed mice that transduced with a reporter gene enabling us to live-image PKM splicing regulation.
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