Caspase 3 Silencing Inhibits Biomechanical Overload-induced Intervertebral Disc Degeneration
Project/Area Number |
23592150
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Hokkaido University |
Principal Investigator |
SUDO HIDEKI 北海道大学, 医学(系)研究科(研究院), 特任准教授 (30374367)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | アポトーシス / 椎間板 / 変性制御 / 椎間板組織 / 生体力学 |
Research Abstract |
Intervertebral disc (IVD) degeneration causes debilitating low back pain in large sections of the worldwide population. No efficient treatment currently exists because the unclear pathogenesis. One characteristic event early in the degeneration is the apoptosis of nucleus pulposus (NP) cells embedded in IVD. Excessive biomechanical loading may be also a major etiology of IVD degeneration. The present study used in vitro and in vivo models of compressive loading to elucidate the underlying mechanism of IVD degeneration. In addition, we investigated whether the inhibition of apoptosis is a potential clinical therapeutic strategy for the treatment of IVD degeneration induced by biomechanical stress.The present study suggests that caspase 3 siRNA attenuates overload-induced IVD degeneration by inhibiting NP cell apoptosis and the expression of matrix-degrading enzymes.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Caspase 3 silencing inhibits biomechanical overload-induced intervertebral disc degeneration2014
Author(s)
Yamada K, Sudo H, Iwasaki K, Sasaki N, Higashi H, Kameda Y, Ito M, Takahata M, Abumi K, Minami A, Iwasaki N
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Journal Title
American Journal of Pathology
Volume: 184
Pages: 753-64
Related Report
Peer Reviewed
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[Journal Article] Caspase 3 Silencing Inhibits Biomechanical Overload-Induced Intervertebral Disk Degeneration2014
Author(s)
Yamada K., Sudo H., Iwasaki K., Sasaki N., Higashi H., Kameda Y., Ito M., Takahata M., Abumi K., Minami A., Iwasaki N.
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Journal Title
Am. J. Pathol.
Volume: 184
Issue: 3
Pages: 753-764
DOI
Related Report
Peer Reviewed
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[Journal Article] A Replication Study for Association of 5 Single Nucleotide Polymorphisms With Curve Progression of Adolescent Idiopathic Scoliosis in Japanese Patients2013
Author(s)
Ogura Y, Takahashi Y, Kou I, Ito M, Sudo H, Kotani T, Watanabe K, Chiba K, Toyama Y, Matsumoto M, Ikegawa S
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Journal Title
Spine (Phila Pa 1976)
Volume: 13
Pages: 571-575
NAID
Related Report
Peer Reviewed
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[Presentation] Therapeutic potential of caspase 3 silencing for biomechanical overload-induced intervertebral disc degeneration. Orthopaedic Research Society2014
Author(s)
Yamada K, Sudo H, Iwasaki K, Sasaki N, Higashi H, Kameda Y, Ito M, Takahata M, Abumi K, Minami A, Iwasaki N
Organizer
2014 annual meeting of the Orthopaedic 8Research Society
Place of Presentation
Hyatt Regency New Orleans /New Orleans, LO, USA
Year and Date
2014-03-15
Related Report
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[Presentation] Therapeutic Potential of Caspase 3 Silencing for Biomechanical Overload-induced Intervertebral Disc Degeneration2014
Author(s)
Yamada K, Sudo H, Iwasaki K, Sasaki N, Higashi H, Kameda Y, Ito M, Takahata M, Abumi K, Minami A, Iwasaki N
Organizer
Orthopaedic Research Society
Place of Presentation
Hyatt Regency New Orleans /New Orleans, LO, USA
Related Report
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