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Regulation of the chondroitin sulfate and recovery systems from the spinal cord injury.

Research Project

Project/Area Number 23592161
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionNiigata University

Principal Investigator

TAKEUCHI Kosei  新潟大学, 医歯(薬)学総合研究科, 客員研究員 (90206946)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords脊髄損傷 / 再生医療 / グリコサミノグリカン / 神経再生 / バイオマテリアル / 核酸医薬 / 神経発生 / 神経科学
Research Abstract

Extracellular factors that inhibit axon growth and intrinsic factors that promote it affect neural regeneration. Therapies targeting any single gene have not yet simultaneously optimized both types of factors. Chondroitin sulphate (CS), a glycosaminoglycan, is the most abundant extracellular inhibitor of axon growth. Here we show that mice carrying a gene knockout for CS N-acetylgalactosaminyltransferase-1 (T1), a key enzyme in CS biosynthesis, recover morecompletely from spinal cord injury than wild-type mice and even chondroitinase ABC-treated mice.Taken together, our results indicate that regulation of a single gene, T1, mediates excellent recovery from spinal cord injury by optimizing counteracting effectorsof axon regeneration

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (54 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (37 results) (of which Invited: 4 results) Book (2 results) Remarks (3 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 2 results)

  • [Journal Article] Pioneering Axons Regulate Neuronal Polarization in the Developing Cerebral Cortex2014

    • Author(s)
      Takashi Namba,Yuji Kibe, Yasuhiro Funahashi, Shinichi Nakamuta, Tetsuya Takano, Takuji Ueno, Akiko Shimada, Sachi Kozawa, Mayumi Okamoto,Yasushi Shimoda,Kanako Oda,Yoshino Wada,Tomoyuki Masuda, Akira Sakakibara,Michihiro Igarashi,Takaki Miyata,Catherine Faivre-Sarrailh, Kosei Takeuchi, Kozo Kaibuchi
    • Journal Title

      Neuron

      Volume: 81 Pages: 814-829

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pioneering Axons Regulate Neuronal Polarization in the Developing Cerebral Cortex.2014

    • Author(s)
      Namba T, Kibe Y, Funahashi Y, Nakamuta S, Takano T, Ueno T, Shimada A, Kozawa S, Okamoto M, Shimoda Y, Oda K, Wada Y, Masuda T, Sakakibara A, Igarashi M, Miyata T, F-S Catherine, Takeuchi K, Kaibuchi K.
    • Journal Title

      Neuron

      Volume: 81(4) Issue: 4 Pages: 814-829

    • DOI

      10.1016/j.neuron.2013.12.015

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chondroitin sulphate N-acetyl galactosaminyl-transferase-1 inhibits recovery from neural injury2013

    • Author(s)
      Kosei Takeuchi, Nozomu Yoshioka, Susumu Higa-Onaga, Yumi Watanabe, Shinji Miyata, Yoshino Wada, Chika Kudo, Masayasu Okada, Kentaro Ohko, Kanako Oda, Toshiya Sato, Minesuke Yokoyama, Natsuki Matsushita, Masaya Nakamura, Hideyuki Okano, Kenji Sakimura,Hitoshi Kawano, Hiroshi Kitagawa, Michihiro Igarashi
    • Journal Title

      Nature Communications

      Volume: 4 Pages: 2740-2740

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Point Mutation in Syntaxin-1A Causes Abnormal Vesicle Recycling, Behaviors, and Short Term Plasticity2013

    • Author(s)
      Yumi Watanabe, Norikazu Katayama, Kosei Takeuchi, Tetsuya Togano, Rieko Itoh, Michiko Sato, Maya Yamazaki, Manabu Abe, Toshiya Sato, Kanako Oda, Minesuke Yokoyama, Keizo Takao, Masahiro Fukaya, Tsuyoshi Miyakawa, Masahiko Watanabe, Kenji Sakimura, Toshiya Manabe, Michihiro Igarashi
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 288(48) Pages: 34906-34919

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding2013

    • Author(s)
      Mayumi Okamoto, Takashi Namba, Tomoyasu Shinoda, Takefumi Kondo, Tadashi Watanabe, Yasuhiro Inoue, Kosei Takeuchi, Yukiko Enomoto, Kumiko Ota, Kanako Oda, Yoshino Wada, Ken Sagou, Kanako Saito, Akira Sakakibara, Ayano Kawaguchi, Kazunori Nakajima, Taiji Adachi, Toshihiko Fujimori, Masahiro Ueda, Shigeo Hayashi, Kozo Kaibuchi, Takaki Miyata
    • Journal Title

      Nature Neuroscience

      Volume: 16(11) Pages: 1556-1566

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury.2013

    • Author(s)
      Kosei Takeuchi, Nozomu Yoshioka, Susumu Higa Onaga, Yumi Watanabe, et. al.
    • Journal Title

      Nature Communications

      Volume: 4 Issue: 1 Pages: 2740-2740

    • DOI

      10.1038/ncomms3740

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Point Mutation in Syntaxin-1A Causes Abnormal Vesicle Recycling, Behaviors, and Short-term Plasticity.2013

    • Author(s)
      Yumi Watanabe, Norikazu Kata yama, Kosei Takeuchi, et. al.
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 48 Pages: 34906-34919

    • DOI

      10.1074/jbc.m113.504050

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of the dorsal ramus of the spinal nerve in the chick embryo : A close relationship between development and expression of guidance cues2012

    • Author(s)
      Masuda T., Sakuma C., Taniguchi M., Kanemoto A., Yoshizawa M., Satomi K., Tanaka H., Takeuchi K., Yaginuma H., Shiga T
    • Journal Title

      Brain Research

      Volume: 1480 Pages: 30-40

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of the dorsal ramus of the spinal nerve in the chick embryo: A close relationship between development and expression of guidance cues.2012

    • Author(s)
      Masuda T., Sakuma C., Taniguchi M., Kanemoto A., Yoshizawa M., Satomi K., Tanaka H., Takeuchi K., Yaginuma H., Shiga T.
    • Journal Title

      Brain Res.

      Volume: 1480 Pages: 30-40

    • DOI

      10.1016/j.brainres.2012.08.055

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 神経極性決定に関与する4回膜貫通glycoprotein M6aの機能解析2013

    • Author(s)
      武内恒成、本多敦子、伊藤泰行、峯田克彦、五十嵐道弘
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(兵庫)
    • Year and Date
      2013-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] CSGalNAcT1, an Enzyme Required for Chondroitin Sulfate Synthesis2013

    • Author(s)
      武内恒成、吉岡望、岡田正康、五十嵐道弘
    • Organizer
      is Critical to Recovery from Neural Injury「Neuroscience2013
    • Place of Presentation
      San Diego Convention Center(サンディエゴ・アメリカ)
    • Year and Date
      2013-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] New trial of gene knockdown system by the siRNA-absorbent silk sponge to recovery from spinal cord injury2013

    • Author(s)
      武内恒成
    • Organizer
      AMWC(Advanced Materials World Congress 2013
    • Place of Presentation
      イズミル・トルコ
    • Year and Date
      2013-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] コンドロイチン硫酸合成酵素欠損マウスにおける神経発生・発達異常2013

    • Author(s)
      吉岡望、比嘉進、武内恒成、五十嵐道弘
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2013-09-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Abnormal brain development/maturation in mice lacking in chondroitin sulfate - synthesizing enzymes2013

    • Author(s)
      武内恒成
    • Organizer
      ECMNET(European cooeration in science and technology)
    • Place of Presentation
      オパレニカ・ポーランド
    • Year and Date
      2013-09-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 長円錐における網羅的なリン酸化タンパク質の探索2013

    • Author(s)
      河嵜麻実、武内恒成、野住素広、五十嵐道弘成
    • Organizer
      Neuro2013 (第36回日本神経科学大会、第56回日本神経化学会大会、第23回日本神経回路学会大会)
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2013-06-22
    • Related Report
      2013 Final Research Report
  • [Presentation] コンドロイチン硫酸合成酵素欠損マウスにおける神経発生・発達異常2013

    • Author(s)
      吉岡望、武内恒成、岡田正康、和田芳野、川野仁、五十嵐道弘
    • Organizer
      Neuro2013 (第36回日本神経科学大会、第56回日本神経化学会大会、第23回日本神経回路学会大会)
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2013-06-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 神経成長円錐マーカー分子GAP43新規リン酸化ドメインとその認識抗体の応用2013

    • Author(s)
      河嵜麻実、野住素広、武内恒成、五十嵐道弘
    • Organizer
      第35回神経組織培養研究会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪)
    • Year and Date
      2013-06-03
    • Related Report
      2013 Final Research Report
  • [Presentation] Brain development/ maturation in mice lacking in chondroitin sulfate synthesizing enzymes2013

    • Author(s)
      吉岡望、武内恒成、五十嵐道弘
    • Organizer
      25th国際神経化学学会(ISN)/米国神経化学学会(ASN) (ISN2013 Glial Satellite)
    • Place of Presentation
      Hotel El Castellano(カンクン・メキシコ)
    • Year and Date
      2013-04-19
    • Related Report
      2013 Final Research Report
  • [Presentation] グリコサミノグリカン発現制御による神経再生・発生2013

    • Author(s)
      武内恒成、吉岡望、比嘉進、川野仁、五十嵐道弘
    • Organizer
      第118回日本解剖学会総会
    • Place of Presentation
      サンポートホール高松・香川国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] CSGalNAcT1, an Enzyme Required for Chondroitin Sulfate Synthesis, is Critical to Recovery from Neural Injury2013

    • Author(s)
      武内恒成
    • Organizer
      FENS meeting
    • Place of Presentation
      プラハ(チェコ)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Abnormal brain development/maturation in mice lacking in chondroitin sulfate - synthesizing enzymes2013

    • Author(s)
      武内恒成
    • Organizer
      ECMNET(European cooeration in science and technology)
    • Place of Presentation
      オパレニカ(ポーランド)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] New trial of gene knockdown system by the siRNA-absorbent silk sponge to recovery from spinal cord injury2013

    • Author(s)
      武内恒成
    • Organizer
      AMWC(Advanced Materials World Congress 2013)
    • Place of Presentation
      イズミル(トルコ)
    • Related Report
      2013 Annual Research Report
  • [Presentation] CSGalNAcT1, an Enzyme Required for Chondroitin Sulfate Synthesis, is Critical to Recovery from Neural Injury2013

    • Author(s)
      武内恒成
    • Organizer
      Neuroscience2013
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 神経極性決定に関与する4回膜貫通glycoprotein M6aの機能解析2013

    • Author(s)
      武内恒成
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] グリコサミノグリカン発現制御による神経再生・発生2013

    • Author(s)
      武内恒成
    • Organizer
      第118回日本解剖学会総会
    • Place of Presentation
      香川
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] コンドロイチン硫酸はマウス視覚野においてOtx2取り込みと臨界期可塑性を制御する2012

    • Author(s)
      候旭濱、武内恒成、五十嵐道弘、杉山清佳
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Brain development abnormality and axon regeneration in the mice lacking in the enzymes synthesizing chondroitin sulfate2012

    • Author(s)
      武内恒成.
    • Organizer
      第45回日本発生生物学会・第64回日本細胞生物学会合同大会
    • Place of Presentation
      神戸国際センター
    • Related Report
      2013 Final Research Report
  • [Presentation] コンドロイチン硫酸の発現制御による神経再生機構2012

    • Author(s)
      武内恒成、吉岡望、渡部裕美、川嵜麻実、和田芳野、五十嵐道弘
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] 損傷脳におけるコンドロイチン硫酸プロテオグリカンの産生メカニズム2012

    • Author(s)
      吉岡望、武内恒成、阿相皓晃、黒田純子、五十嵐道弘、川野仁
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] Cre/loxP組換え依存の標的遺伝子ノックダウン誘導のためのレンチウイルスベクターシステム2012

    • Author(s)
      松下夏樹、松下佐知、武内恒成、八十島安伸、東山繁樹
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] Axon regeneration in the mice lacking in the enzymes synthesizing chondroitin sulfate2012

    • Author(s)
      武内恒成、吉岡望、川野仁、五十嵐道弘
    • Organizer
      8^<th> Forum of neuroscience
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] Brain development abnormality and axon regeneration in the mice lacking in the enzymes synthesizing chondroitin sulfate2012

    • Author(s)
      武内恒成
    • Organizer
      2^<nd> Annual Conference of COST Action ECMNet"Brain extracellular matrix in health and disease
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] プロテオグリカン合成系の調節による神経損傷修復機構2012

    • Author(s)
      武内恒成、吉岡望、比嘉進、渡邊裕美、五十嵐道弘、川野仁
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] イムノグロブリン様細胞接着分子による生体内での軸索形成制御2012

    • Author(s)
      木部祐士、難波隆志、舟橋靖広、中牟田信一、武内恒成、貝淵弘三
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] Axon regeneration in the mice lacking in the enzymes synthesizing chondroitin sulfate.2012

    • Author(s)
      Kosei Takeuchi, Yoshioka Nozomu, Susumu Higa, Hitoshi Kawano, Michihiro Igarashi
    • Organizer
      8th Forum of European Neurosciences (FENS meetings 2012)
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2012 Research-status Report
  • [Presentation] Brain development abnormality and axon regeneration in the mice lacking in the enzymes synthesizing chondroitin sulfate2012

    • Author(s)
      Kosei Takeuchi
    • Organizer
      2nd Annual Conference of COST action ECMNet
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Cre/loxP組換え依存の標的遺伝子ノックダウン誘導のためのレンチウイルスベクターシステム2012

    • Author(s)
      松下夏樹、松下佐知、武内恒成、八十島安伸、東山繁樹
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] コンドロイチン硫酸はマウス視覚野においてOtx2取り込みと臨界期可塑性を制御する2012

    • Author(s)
      候旭濱、武内恒成、五十嵐道弘、杉山清佳
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] コンドロイチン硫酸の発現制御による神経再生機構2012

    • Author(s)
      武内恒成、比嘉進、吉岡望、渡部裕美、川嵜麻実、和田芳野、五十嵐道弘
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] コンドロイチン硫酸合成酵素KOマウスにおける機能解析2011

    • Author(s)
      武内 恒成
    • Organizer
      第30回日本糖質科学会年会(招待講演)
    • Place of Presentation
      ハイブ長岡(新潟県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Brain development abnormality and axon regeneration in the mice lacking in the enzymes synthesizing chondroitin sulfate2011

    • Author(s)
      武内 恒成
    • Organizer
      8th IBRO World Congress of Neurosciences
    • Place of Presentation
      Fortezza de Basso Convention, (Florence, Italy)
    • Related Report
      2011 Research-status Report
  • [Presentation] Cre/loxP 相同組換えに依存するRNAi誘導ベクターの構築と評価2011

    • Author(s)
      松下 夏樹
    • Organizer
      第3回日本RNAi研究会大会
    • Place of Presentation
      グランドプリンス広島(広島県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Development of lentiviral vector systems for conditional gene knockdown induced by Cre/loxP recombination2011

    • Author(s)
      松下 夏樹
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] コンドロイチン硫酸合成に関する遺伝子改変マウスの神経系発生解析2011

    • Author(s)
      武内 恒成
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] コンドロイチン硫酸合成酵素ノックアウトマウスは脊髄損傷回復が早い2011

    • Author(s)
      武内 恒成
    • Organizer
      第54回日本神経化学会大会
    • Place of Presentation
      山代温泉瑠璃光(石川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Mice lacking in an enzyme involved in chondroitin sulfate synthesis shows better recovery from spinal cord injury.2011

    • Author(s)
      武内 恒成
    • Organizer
      Annual Meeting of Society for Neuroscience 2011
    • Place of Presentation
      Walter E Washington Convention Center, (Washington DC, USA)
    • Related Report
      2011 Research-status Report
  • [Book] 遺伝子導入プロトコール-発現解析とRNAi実験-(編集企画を担当及び分担執筆(武内恒成)2012

    • Author(s)
      仲嶋一範、北村義浩、武内恒成
    • Total Pages
      251
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Book] 遺伝子導入プロトコール -発現解析とRNAi実験-2012

    • Author(s)
      仲嶋一範、北村義浩、武内恒成 (3者共同編著)
    • Total Pages
      252
    • Publisher
      羊土社
    • Related Report
      2012 Research-status Report
  • [Remarks] 新聞報道 (新潟日報 朝日新聞など)2013年11月13日

    • Related Report
      2013 Final Research Report
  • [Remarks] プレスリリース記事

    • URL

      http://www.med.niigata-u.ac.jp/bc2/news/press.pdf

    • Related Report
      2013 Annual Research Report
  • [Remarks] 教室研究成果

    • URL

      http://www.med.niigata-u.ac.jp/bc2/study/index.html

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 神経再生発生を可視化する抗リン酸化抗体2014

    • Inventor(s)
      武内恒成
    • Industrial Property Rights Holder
      武内恒成
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-04-18
    • Related Report
      2013 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 抗GAP43抗体2012

    • Inventor(s)
      武内恒成 河嵜麻実 五十嵐道弘
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-10-19
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] GAP43抗体2011

    • Inventor(s)
      五十嵐 道弘、武内 恒成
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Number
      2011-230577
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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