| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
We created an ulnar vessel-containing tube (70% polylactic acid and 30% polycaprolactone), to which bone marrow-derived mesenchymal stem cells (BMCs) were implanted. The tube was transplanted to bridge a 3cm ulnar nerve defect in canines. Nerve regeneration through the tube was compared with that in a 3cm-long autogenous ulnar nerve segment. Although the rate of nerve regeneration in the tube was slower than that in the nerve segment, the histomorphological analysis of nerve regeneration in the tube was almost more than 80% of that in the autogenous nerve segment 24 weeks after surgery. When BMCs stained with CM dye-I were transplanted into the tube chamber, some CM-dye I-positive cells demonstrated S100 and GFAP markers in the nerve regenerated within the tube. These results indicated that some of the BMCs transplanted into the tube differentiated into Schwann cells, which might have facilitated nerve regeneration in the vessel-containing tubes with BMC transplantation.
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