| Project/Area Number |
23592174
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKATSUKA Terumasa 関西医療大学, 保健医療学部, 客員教授 (30380752)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 神経障害性疼痛 / 動物モデル / 後根神経節 / 脊髄後角 / パッチクランプ法 / 腰部脊柱管狭窄症 / 椎間孔部障害 / 椎間孔外側障害 / ダブルクラッシュ / 交感神経 / 疼痛メカニズム / 神経因性疼痛 / ミクログリア |
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| Research Abstract |
The lumbar radiculopathy rat model was created by ligating the dorsal root ganglia (DRG) of the L5 spinal nerve, 2 mm proximal to the DRG or 2 mm distal to the DRG, and the two sites of them (i.e. double crush) with 6.0 silk. We examined the degree of the radiculopathy using mechanical sensitivity, immunohistochemistry and in vivo patch-clamp recordings, 7 days after surgery. The rats injured the DRG was most sensitive, and the rats injured distal to the DRG were more sensitive than those rats injured proximal to the DRG in the behavioral study. The number of activated microglia in laminas I-II of the L5 segmental level and the amplitudes and frequencies of EPSC in the rats increased in the same order with the behavioral study. There was no significant difference between the double crush models and the DRG injured model. Moreover, we found that lumbar sympathectomy decreased the mechanical sensitivity and the frequencies of EPSC in the DRG injured model.
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