| Project/Area Number |
23592198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
OMOKAWA shohei 奈良県立医科大学, 医学部, 准教授 (70597103)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yasuhito 奈良県立医科大学, 整形外科, 教授 (30316070)
WADA Takuro 札幌医科大学, 整形外科, 教授 (00244369)
AKAHANE Manabu 奈良県立医科大学, 健康政策医学, 准教授 (40326327)
SHIMIZU Takamasa 奈良県立医科大学, 整形外科, 研究員 (70464667)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 骨折 / 偽関節 / 再生医療 / 骨芽細胞 / 細胞シート / 筋膜脂肪移植 / 関節拘縮 / 骨芽細胞シート / 間葉系幹細胞 / 大腿骨偽関節 |
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| Research Abstract |
We evaluated whether a percutaneous injection of osteogenic cell sheet (OCSs) could enhance bone regeneration in a nonunion site without adhesions to surrounding structures. Nonunion models were created in the femora of rats using marrow ablation and periosteal removal. The rats were then randomly divided into 3 groups. Six hours after fracture, one group received an injection of OCSs (OCS group), the second, pre-differentiated BMSCs (BMSC group) and the third, PBS (control group). Radiological and histological evaluation demonstrated enhanced bone regeneration in the OCS group as compared with the other groups. Biomechanical testing revealed a significantly higher maximum bending load in the OCS group compared with the other groups. No prophylactic effects were observed in the OCS group for adhesion to surrounding structures. The present study demonstrated that the injection of entire OCSs can enhance bone regeneration, leading to successful bone union of fracture nonunion.
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