| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Siglec-15 is DAP12 associated receptor, which is essential for the formation of functional osteoclast. In this research, we identified a Siglec-15-binding protein that interacts with cytoplasmic region of Siglec-15. Cytoplasmic region of Siglec-15 that associates this protein was essential for the function of Siglec-15. Chimeric molecules consisting of the extracellular region, transmembrane region with a K272A mutation, and the functional region of cytoplasmic tail of Siglec-15 and the cytoplasmic region of DAP12 restore bone resorption in osteoclasts from Siglec-15 knockout mice that were established in this research. These results strongly suggested that Siglec-15 is a critical DAP12-associated receptor in osteoclasts.
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