Functional analysis of novel DAP12-associated receptor in osteoclasts.
| Project/Area Number |
23592214
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 骨・軟骨代謝学 / 破骨細胞 / 骨吸収 / NFATc1 / ITAM / レクチン |
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| Outline of Final Research Achievements |
Siglec-15 is DAP12 associated receptor, which is essential for the formation of functional osteoclast. In this research, we identified a Siglec-15-binding protein that interacts with cytoplasmic region of Siglec-15. Cytoplasmic region of Siglec-15 that associates this protein was essential for the function of Siglec-15. Chimeric molecules consisting of the extracellular region, transmembrane region with a K272A mutation, and the functional region of cytoplasmic tail of Siglec-15 and the cytoplasmic region of DAP12 restore bone resorption in osteoclasts from Siglec-15 knockout mice that were established in this research. These results strongly suggested that Siglec-15 is a critical DAP12-associated receptor in osteoclasts.
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Report
(5 results)
Research Products
(14 results)
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[Journal Article] Cyclodextrin Complexed [60]Fullerene Derivatives with High Levels of Photodynamic Activity by Long Wavelength Excitation.2013
Author(s)
Ikeda A, Iizuka T, Maekubo N, Aono R, Kikuchi J, Akiyama M, Konishi T, Ogawa T, Ishida-Kitagawa N, Tatebe H, Shiozaki K
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Journal Title
ACS Medicinal Chemistry Letters
Volume: 4
Issue: 8
Pages: 752-756
DOI
Related Report
Peer Reviewed
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[Journal Article] Aberrant gene expression and sexually incompatible genomic imprinting in oocytes derived from XY mouse embryonic stem cells in vitro.2013
Author(s)
Nitta M, Imamura M, Inoue Y, Kunitomo Y, Lin ZY, Ogawa T, Yogo K, Ishida-Kitagawa N, Fukunaga N, Okano H, Sato E, Takeya T, Miyoshi J
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Journal Title
PLoS One
Volume: 8
Issue: 3
Pages: e58555-e58555
DOI
Related Report
Peer Reviewed
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[Journal Article] Siglec-15 protein regulates the formation of functional osteoclasts in concert with DNAX-activating protein of 12 KDa (DAP12).2012
Author(s)
Ishida-Kitagawa N, Tanaka K, Bao X, Kimura T, Miura T, Kitaoka Y, Hayashi K, Sato M, Maruoka M, Ogawa T, Miyoshi J, Takeya T.
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Journal Title
The Journal of Biological Chemistry
Volume: in press
Related Report
Peer Reviewed
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