Investigation for gene regulating factors in articular cartilage degeneration

• Project/Area Number: 23592218
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyushu University
• Principal Investigator: OKAZAKI KEN 九州大学, 大学病院, 講師 (10398092)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 関節炎 / 軟骨 / 遺伝子発現 / 転写因子 / 軟骨代謝 / 軟骨分化 / 破骨細胞 / 骨破壊 / 軟骨変性 / 変形性関節症 / 関節リウマチ

Research Abstract

We found that a transcriptional factor, C/EBP plays crucial roles in gene regulation which affect cartilage degeneration in osteoarthritis or rheumatoid arthritis. C/EBP stimulates gene expressions of MMP1, MMP-3, MMP-13, which denature the cartilage matrix. C/EBP also stimulate RANKL expression to promote osteoclast formation that consequently promote bone destruction in rheumatoid arthritis. Furthermore, C/EBP repress the expression of type II collagen which is a major matrix molecule of cartilage.

Research Products

• [Journal Article] CCAAT/enhancer binding proteinβregulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes (2014)
  • Author(s): Ushijima T, Okazaki K, Tsushima H, Iwamoto Y
  • Journal Title: J Biol Chem Volume: 289(5) Pages: 2852-63
• [Journal Article] CCAAT/enhancer binding protein β regulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes (2014)
  • Author(s): Ushijima T, Okazaki K, Tsushima H, Iwamoto Y
  • Journal Title: J Biol Chem Volume: 289 Issue: 5 Pages: 2852-2863
  • DOI: 10.1074/jbc.m113.492843
  • Peer Reviewed
• [Journal Article] T1ρis superior to T2 mapping for the evaluation of articular cartilage denaturalization with osteoarthritis : Radiological-pathological correlation after total knee arthroplasty (2013)
  • Author(s): Takayama Y, Hatakenaka M, Tsushima H, Okazaki K, Yoshiura T, Yonezawa M, Nishikawa K, Iwamoto Y, Honda H
  • Journal Title: Eur J Radiol Volume: 82(4) Issue: 4 Pages: e192-e198
  • DOI: 10.1016/j.ejrad.2012.11.031
  • Peer Reviewed
• [Journal Article] 変形性膝関節症の病態生理と診断 (2013)
  • Author(s): 岡崎賢
  • Journal Title: 整形外科看護 Volume: 18(8) Pages: 722-731
• [Journal Article] CCAAT/enhancer binding proteinβregulates expression of matrix metalloproteinase-3 in arthritis (2012)
  • Author(s): Tsushima H, Okazaki K, Hayashida M, Ushijima T, Iwamoto Y
  • Journal Title: Ann Rheum Dis Volume: 71 Pages: 99-107
  • NAID: 120005324439
• [Journal Article] Evaluation of cartilage degradation in arthritis using T1ρ magnetic resonance imaging mapping (2012)
  • Author(s): Tsushima H, Okazaki K, Takayama Y, Hatakenaka M, Honda H, Izawa T, Nakashima Y, Yamada H, Iwamoto Y
  • Journal Title: Rheumatology international Volume: 32 Issue: 9 Pages: 2867-2875
  • DOI: 10.1007/s00296-011-2140-3
  • Peer Reviewed
• [Journal Article] 関節リウマチ・変形性関節症の軟骨変性における遺伝子発現の制御 (2012)
  • Author(s): 岡崎賢, 岩本幸英
  • Journal Title: 福岡医学雑誌 Volume: 103(9) Pages: 175-180
  • NAID: 40019478942
• [Journal Article] CCAAT/enhancer binding protein β regulates expression of matrix metalloproteinase-3 in arthritis (2012)
  • Author(s): Tsushima H, Okazaki K, Hayashida M, Ushijima T, Iwamoto Y
  • Journal Title: Annals of Rheumatic Diseases Volume: 71 Issue: 1 Pages: 99-107
  • DOI: 10.1136/annrheumdis-2011-200061
  • NAID: 120005324439
  • Peer Reviewed
• [Presentation] 軟骨分化におけるCCAAT/enhancer-binding proteinβの関わり (2014)
  • Author(s): 岡崎賢、牛島貴宏、津嶋秀俊、林田光正、岩本幸英
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] 軟骨分化におけるCCAAT/enhancer-binding protein βの関わり (2014)
  • Author(s): 岡崎 賢、牛島貴宏、津嶋秀俊、林田光正、岩本幸英
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都市
  • Invited
• [Presentation] 軟骨分化においてCCAAT/enhancer-binding protein βはindian hedgehogの発現を調節する (2014)
  • Author(s): 牛島貴宏、岡崎 賢、津嶋秀俊、岩本幸英
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] CCAAT/enchancer-binding protein beta promotes osteoclast differentiation through RANKL expression from synovial fibroblast like cells in rheumatoid arthritis (2013)
  • Author(s): Tsushima H, Okazaki K, Ushijima T, Iwamoto Y
  • Organizer: 2013 annal meeting of orthopaedic research society
  • Place of Presentation: San Antonio, TX, USA
• [Presentation] CCAAT/enhancer-binding protein beta regulates phenotypic conversion from proliferative to hypertrophic chondrocytes during endochondral ossification (2013)
  • Author(s): Ushijima T, Okazaki K, Tsushima H, Iwamoto Y
  • Organizer: 2013 annual meeting of orthopaedic research society
  • Place of Presentation: San Antonio, TX, USA
• [Presentation] 関節炎における転写制御と軟骨破壊 (2011)
  • Author(s): 岡崎賢、津嶋秀俊、牛島貴宏、岩本幸英
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋市
• [Presentation] Gene regulation of cartilage degeneration in osteoarthritis (2011)
  • Author(s): Okazaki K, Tsushima H, Hayashida M, Ushijima T, Iwamoto Y
  • Organizer: 第24回日本軟骨代謝学会
  • Place of Presentation: 福岡市
• [Presentation] 関節炎における転写制御と軟骨破壊 (2011)
  • Author(s): 岡崎 賢、津嶋秀俊、牛島貴宏、岩本幸英
  • Organizer: 第26回日本整形外科学会基礎学術集会(招待講演)
  • Place of Presentation: 前橋市