Construction of a model mouse library related to osteogenic and chondrogenic disease using an exchangeable gene trap mutagenesis
| Project/Area Number |
23592220
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
CHOSA Etsuo 宮崎大学, 医学部, 教授 (00236837)
ARAKI Masatake 熊本大学, 生命資源研究・支援センター, 准教授 (80271609)
ARAKI Kimi 熊本大学, 生命資源研究・支援センター, 准教授 (90211705)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 遺伝子トラップ法 / ロコモティブシンドローム / 骨軟骨代謝 / 新規遺伝子群 / モデルマウス / スクリーニング / マウスライブラリー / EGTC / 骨・軟骨代謝 / ライブラリー |
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| Research Abstract |
Gene trapping in ES cells is a proven method for large-scale random insertional mutagenesis in the mouse genome. We have established an exchangeable gene trap system, in which a reporter gene can be exchanged for any other DNA of interest through Cre/mutant lox-mediated recombination. We isolated trap clones, analyzed trapped genes, and constructed the database for Exchangeable Gene Trap Clones (EGTC) [http://egtc.jp]. The EGTC database is the most extensive academic resource for gene-trap mouse lines. The number of registered ES cell lines was 1200 on March 2014. We also established 450 mouse lines from trap ES clones and deposited them in the mouse embryo bank at the CARD, Kumamoto Univ., Japan. Using EGTC database, we established 45 mouse lines related to osteogenic and chondrogenic metabolism. We analyzed the bone and cartilage phenotypes using micro CT, bone morphometry, biomechanical analyses, realtime-PCR, etc. Thirty seven mouse lines showed some bone and/or cartilage disorder.
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Report
(4 results)
Research Products
(26 results)
