| Project/Area Number |
23592235
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Yasuchika 東邦大学, 医学部, 准教授 (70584001)
NAKAJIMA Arata 東邦大学, 医学部, 准教授 (60583995)
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| Co-Investigator(Renkei-kenkyūsha) |
SASHO Takahisa 千葉大学, 大学院医学研究院, 准教授 (20312952)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 関節病学 / 軟骨再生 / 滑膜細胞 / 滑膜 / ヒト |
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| Research Abstract |
[Objective] To examine the effect of co-culture or co-implantation of synovial and minced cartilage on the chondrogenic differentiation. [Methods] Human synovial cells were isolated and embedded in fibrin gel. The fibrin gel implants containing only synovial cells (SC group) or both minced articular cartilage and synovial cells (AC+SC group) were cultured or implanted subcutaneously in nude mice and then examined the histological and biochemically. [Results] Intensity of Safranin-O staining and proteoglycan content (per weight) increased in the AC+SC group compared to the SC group. Furthermore, type II collagen and Sox9 expression were enhanced in the AC+SC group. [Conclusion] Co-culture or co-implantation of synovial cells with minced cartilage enhanced chondrogenic differentiation. The use of implant grafts containing both synovial cells and minced cartilage could be a novel approach for one-step joint cartilage repair.
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