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Optical image analysis of anesthetic action on regional brain function and preparation of the video library of neuronal activities

Research Project

Project/Area Number 23592244
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNiigata University

Principal Investigator

FUJIWARA Naoshi  新潟大学, 医歯学系, 教授 (70181419)

Co-Investigator(Kenkyū-buntansha) SEO Kenji  新潟大学, 医歯学系, 教授 (40242440)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords神経活動画像解析 / 膜電位画像 / カルシウム画像 / 大脳皮質 / 海馬 / 脳スライス / 細胞内カルシウム画像 / 神経伝達 / 脳局所機能 / GABAA受容体
Research Abstract

Optical imaging of neuronal functions, e.g., membrane excitation and intracellular calcium changes, in slices of the mouse cerebral cortex and hippocampus was performed using an imaging system equipped with a high-speed camera. The imaging applied to the characterization of neuronal functions under various conditions. Responses of the membrane excitation and intracellular calcium changes to stimulation were sensitively changed under various conditions of perfused solutions, particularly by temperature. Anesthetic actions of anesthetics on regional brain functions were referred to the location of the GABAA receptors. Different effect of anesthetics on the cortex and hippocampus may relate to the differences in localization of the GABAA receptor subtypes.
The video images of neuronal functions obtained in this research project were collected and edited to produce a video library. The video images are up-loaded on the following website. URL: http://www.neuro-animation.jp/

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2012 2011 Other

All Journal Article (1 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] Effects of acid-base balance on intracellular calcium changes in response to neuronal excitation in brain slices of mice2011

    • Author(s)
      Fujiwara N, Sato R, Takenoshita K, Nagayama H, Seo K
    • Journal Title

      Neurosurg Anesthesiol

      Volume: 22 (4) Pages: 457-457

    • Related Report
      2013 Final Research Report
  • [Presentation] 脳切片を用いた局所脳機能の画像解析;低温でのシグナル伝達2012

    • Author(s)
      菅田英樹,松田将門,藤原直士
    • Organizer
      第75回新潟麻酔懇話会・第54回新潟ショックと蘇生・集中治療研究会
    • Place of Presentation
      新潟市
    • Year and Date
      2012-06-02
    • Related Report
      2013 Final Research Report
  • [Presentation] バルビタール系麻酔薬の局所脳機能抑制作用とGABAA受容体β2,β3サブユニットの分布2012

    • Author(s)
      松田将門,菅田英樹,瀬尾憲司,藤原直士
    • Organizer
      第75回新潟麻酔懇話会・第54回新潟ショックと蘇生・集中治療研究会
    • Place of Presentation
      新潟市
    • Year and Date
      2012-06-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 脳切片を用いた局所脳機能の画像解析;低温でのシグナル伝達2012

    • Author(s)
      菅田英樹、松田将門、藤原直士
    • Organizer
      第75回新潟麻酔懇話会、第54回新潟ショックと蘇生・集中治療研究会
    • Place of Presentation
      新潟市
    • Related Report
      2012 Research-status Report
  • [Presentation] バルビタール系麻酔薬の局所脳機能抑制作用とGABA(A)受容体β2, β3サブユニットの分布2012

    • Author(s)
      松田将門、菅田英樹、瀬尾憲司、藤原直士
    • Organizer
      第75回新潟麻酔懇話会、第54回新潟ショックと蘇生・集中治療研究会
    • Place of Presentation
      新潟市
    • Related Report
      2012 Research-status Report
  • [Remarks] 本研究による神経活動の動画サイト

    • URL

      http://www.neuro-animation.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] http://www.neuro-animation.jp/

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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