| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The neurotoxic effects of anesthetics on the developing brain are a concern.
We examined the degree of L-glutamate-induced intracellular calcium ([Ca2+]i) elevation and neurotoxicity in primary cultured neurons exposed to anesthetics.The primary cortical neurons from E17 Wistar rats were preincubated with various concentrations of ketamine or thiopental sodium (TPS) for the first 72 h of culturing. Two weeks later, the neurons were exposed to L-glutamate. The extent of glutamate toxicity was evaluated by Ca-imaging and morphological experiments.Although preincubation with extra high concentration of ketamine showed enhancement of L-glutamate-induced [Ca2+]i elevation 2 weeks later, long-term exposure to TPS or ketamine at clinical dose during developmental periods may not result in a dose-related potentiation of exogenous glutamate-induced neurotoxicity, once the intravenous anesthetics are removed.
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