The role of survival proteins involved in myocardial dysfunction after.

• Project/Area Number: 23592254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka University
• Principal Investigator: HAYASHI Yukio 大阪大学, 医学(系)研究科(研究院), 准教授 (60294063)
• Co-Investigator(Kenkyū-buntansha): KAMIBASHI Takahiko 大阪大学, 医学系研究科, 講師 (10273640)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳死 / 心機能 / フォスフォイノシトール3キナーゼ / Akt / フォスフォランバン / β2受容体 / サバイバル蛋白

Research Abstract

We examined the mechanism involved in myocardial dysfunction following brain death using rat-brain-death-model. Brain death was produced by graded inflation of a 4Fr Fogarty catheter inserted into the animal's subdural space by 300 ul of saline. The study was designed to examine the Phosphatidylinositol 3-kinase (PI3K)-Akt signaling involved in the myocardial dysfunction after brain death. Wortmannin, an inhibitor of PI3K, improved EF and LV dp/dt max and survival rate after brain death. Another study using Western blotting showed that brain death increased phosphorylation of Akt and decreased phosphorylation of phosphoramban and wortmannin suppressed the phosphorylation of Akt and reversed the decreased phosphorylation of phosphoramban. In addition, wortmannin did not affect of level of SERCAs. These data indicate that myocardial PI3k-Akt-signaling phosphorylation and reduction of phosphorylation of phosphoramban may be involved in the myocardial dysfunction following brain death.

Research Products

• [Journal Article] 脳死ドナーの管理(臓器摘出にかかわる全身管理) (2013)
  • Author(s): 林行雄、本田絢子
  • Journal Title: 麻酔 Volume: 62
• [Journal Article] Nicorandil preserves myocardial function following brain death in rats by mitochondrial adenosine triphosphate- sensitive potassium channel-dependent mechanism (2011)
  • Author(s): Iwasaki M, Hayashi Y, Yamanaka H, Kamibayashi T, Mashimo T
  • Journal Title: Eur J Cardiothorac Surg Volume: 40 Pages: 710-714
• [Journal Article] Nicorandil preserves myocardial function ---- (2011)
  • Author(s): Iwasaki M, Hayashi Y, Yamanaka H, Kamibayashi T,
  • Journal Title: Eur J of Cardio-thoracic Surgery Volume: 40 Pages: 710-4
  • Peer Reviewed
• [Presentation] Involvement of Phosphatidylinositol 3-kinase and Akt signaling in myocardial dysfunction following brain death in rats (2013)
  • Author(s): Satoh M, Hayashi Y, Yamanaka H, Miyata Y, Kamibayashi T, Fujino Y
  • Organizer: The annual meeting of the American Society of Anesthesiologists
  • Place of Presentation: San Francisco, Calfornia
• [Presentation] Involvement of Phosphatidylinositol 3-kinase and Akt signaling in myocardial dysfunction following brain death in rats. (2013)
  • Author(s): Sato M, Hayashi Y, Yamanaka H, Miyata Y, Kamibayashi T, Fujino Y.
  • Organizer: American Society of Anesthesioligy
  • Place of Presentation: Moscone Convention Center(USA)