The search for sterss-induced conditioning: effects of acute hypercapnia

• Project/Area Number: 23592257
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagasaki University
• Principal Investigator: HARA Tetsuya 長崎大学, 医歯(薬)学総合研究科, 教授 (50304952)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 心筋虚血 / コンディショニング / 高炭酸ガス血症 / 気絶心筋 / 高二酸化炭素血症 / 心筋虚血再灌流傷害 / 心筋スタニング / 虚血耐性 / プレコンディショニング / ポストコンディショニング / 周術期管理

Research Abstract

We studied the cardioprotective effect of acute hypercapnia before ischemia or during reperfusion (pre- or post-conditioning) in swine stunned myocardium. Hypercapnia was induced by hypoventilation of PaCO2 70 mmHg for 30 minutes. Stunning was produced by 12-min ischemia and 90-min reperfusion. Myocardial contractility was evaluated with segment shortening. Data were analyzed with Mann-Whitney U test. p<0.05 was considered statistically significant. PaCO2 and coronary blood flow during hypercapnia were significantly higher than those in control swine. There was no significant recovery of stunning. Interleukin after reperfusion were slightly attenuated in pre-conditioned swine. KATP channels were not activated during hypercapnia. Acute hypercapnia could not enhance recovery of stunned myocardium. Attenuation of the preconditioning stimuli by the increase of coronary blood flow would be considered as the result, and therefore enough conditioning stimuli would not be obtained.

Research Products

• [Presentation] 高二酸化炭素血症が気絶心筋の回復に与える影響 (2014)
  • Author(s): 山本裕梨、荒木寛、諸岡秋葉、穐山大治、吉富修、原哲也
  • Organizer: 日本麻酔科学会第61回学術集会
  • Place of Presentation: 横浜市
• [Presentation] 再灌流時の高二酸化炭素血症がブタ気絶心筋の回復に与える影響 (2014)
  • Author(s): 原哲也、一ノ宮大雅、松本周平、趙成三
  • Organizer: 第35回日本循環制御医学会総会
  • Place of Presentation: 福岡市
• [Presentation] Effects of acute hypercapnia in stunned myocardium in swine (2014)
  • Author(s): Tetsuya Hara, Daiji Akiyama, Osamu Yoshitomi
  • Organizer: Annual Meeting of American Society of Anesthesiologists
  • Place of Presentation: New Orleans. USA (Submitted)
• [Presentation] 高二酸化炭素血症が気絶心筋の回復に与える影響 (2014)
  • Author(s): 山本裕梨、荒木寛、諸岡秋葉、穐山大治、吉富修、原哲也
  • Organizer: 日本麻酔科学会第61回学術集会
  • Place of Presentation: パシフィコ横浜展示ホール（横浜市西区）
• [Presentation] 再灌流時の高二酸化炭素血症がブタ気絶心筋の回復に与える影響 (2014)
  • Author(s): 原哲也、一ノ宮大雅、松本周平、趙成三
  • Organizer: 第35回日本循環制御医学会総会
  • Place of Presentation: 九州大学医学部百年講堂（福岡市東区）