Neuroprotective effect of fructose water-soluble alpha-lipoic acid: a 31P-NMR study

• Project/Area Number: 23592259
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Oita University
• Principal Investigator: KITANO Takaaki 大分大学, 医学部, 教授 (20211196)
• Co-Investigator(Kenkyū-buntansha): TOKUMARU Osamu 大分大学, 医学部, 准教授 (40360151) ; YOKOI Isao 大分大学, 医学部, 教授 (80150366)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: αリポ酸 / 脳保護 / フリーラジカル / 虚血再灌流負荷 / 核磁気共鳴法 / 電子スピン共鳴法 / ラジカルスカベンジャー / 高エネルギーリン酸 / 脳保護作用 / 高エネルギー燐酸 / スピントラップ / 脳虚血 / クレアチンリン酸 / ATP / 病態生理学

Research Abstract

Despite its strong antioxidative and anti-inflammatory activity, application of alpha-lipoic acid has been limited due to its water-insolubility. The neuroprotective effect of a newly-developed water-soluble derivative of alpha-lipoic acid was studied using 31P-nuclear magnetic resonance spectroscopy. The recovery of high-energy phosphates, ATP and phosphocreatine (energetic buffer of ATP in living cells), was significantly higher when brain slices were superfused with water-soluble alpha-lipoic acid. The electron spin resonance spectroscopy indicated a wide range of free radical scavenging activity of the water-soluble alpha-lipoic acid.

Research Products

• [Journal Article] Neuroprotective effect of water-soluble vitamin E derivatives as radical scavengers/antioxidants. (2014)
  • Author(s): Suezumi K., Yamada K., Tokumaru O., Ogata K., Kitano T., Yokoi I.
  • Journal Title: J Physiol Sci. Volume: 64
• [Journal Article] Spectrum of dose-response relationship for radical scavenging activity of edaravone (2014)
  • Author(s): Tokumaru O, Shuto Y, Ogata K, Yokoi I
  • Journal Title: J Physiol Sci Volume: ６４
• [Journal Article] 虚血-再灌流負荷に対するラットの脳エネルギー代謝の変化 : ^<31>P-NMRによる経時的検討 (2013)
  • Author(s): 徳丸治,北野敬明,横井功
  • Journal Title: 日本生理学会雑誌 Volume: 75(5) (Pt2) Pages: 31-33
• [Journal Article] Neuroprotective effects of a water-solubleα-lipoic acid derivative, DM-His.Zn, on rat brain tissue after ischemia-reperfusion injury : spin resonance analyses study (2013)
  • Author(s): Mizutani Y,Shimada M, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Journal Title: J Physiol Sci Volume: 63
• [Journal Article] Neuroprotective effects of acetyl-L-carnitine on rat brain slices after ischemia-reperfusion injury : spin resonance analyses study (2013)
  • Author(s): Shimada M, Mizutani Y, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Journal Title: J Physiol Sci Volume: 63
• [Journal Article] 虚血－再灌流負荷に対するラットの脳エネルギー代謝の変化：31P-NMRによる経時的検討． (2013)
  • Author(s): 徳丸治，北野敬明，横井功．
  • Journal Title: 日本生理学会雑誌 Volume: 75(5), Pt2 Pages: 31-33
• [Journal Article] Recovery of energy metabolism of rat brain after ischemia-reperfusion injury: a 31P-NMR study (2013)
  • Author(s): Tokumaru O, Kitano T, Yokoi I
  • Journal Title: J Physiol Sci Volume: 63
• [Journal Article] Neuroprotective effects of a water-soluble α-lipoic acid derivative, DM-His.Zn, on rat brain tissue after ischemia-reperfusion injury : spin resonance analyses study (2013)
  • Author(s): Mizutani Y,Shimada M, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Journal Title: J Physiol Sci Volume: 63
• [Journal Article] Neuroprotective effects of acetyl-L-carnitine on rat brain slices after ischemia-reperfusion injury: spin resonance analyses study (2013)
  • Author(s): Shimada M, Mizutani Y, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Journal Title: J Physiol Sci Volume: 63
• [Journal Article] Sexand age-related differences in morbidity rates of 2009 pandemic influenza A H1N1 virus of swine origin in Japan (2010)
  • Author(s): Nobuoki Eshima, Osamu Tokumaru, Shohei Hara, Kira Bacal, Seigo Korematsu, Minoru Tabata, Shigeru Karukaya, Yoshinori Yasui, Nobuhiko Okabe, Toyojiro Matsuishi
  • Journal Title: PLoS ONE Volume: 6(4) Issue: 4 Pages: 1261-1293
  • DOI: 10.1371/journal.pone.0019409
  • URL: http://dx.plos.org/10.1371/journal.pone.0019409
  • Peer Reviewed
• [Presentation] Spectrum of dose-response relationship for radical scavenging activity of edaravone (2014)
  • Author(s): Tokumaru O, Shuto Y, Ogata K, Yokoi I
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
• [Presentation] Neuroprotective effect of water-soluble vitamin E derivatives as radical scavengers/antioxidants (2014)
  • Author(s): Suezumi K, Yamada K, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
• [Presentation] 水溶性ビタミンＥ誘導体の抗酸化能と脳保護作用 (2014)
  • Author(s): 居積晃希，山田訓也，徳丸治，尾方和枝，北野敬明，横井功．
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
• [Presentation] 水溶性αリポ酸誘導体Dm-His.Znのラジカル除去作用 : ESRによる検討 (2013)
  • Author(s): 徳丸治,北野敬明,横井功
  • Organizer: 第4回癌・炎症とαリポ酸研究会
  • Place of Presentation: 大分・日出
  • Year and Date: 2013-11-16
• [Presentation] Neuroprotective effects of acetyl-L-carnitine against ischemia-reperfusion injury : a spin resonance study (2013)
  • Author(s): Tokumaru O, Shimada M, Mizutani Y, Ogata K, Kitano T, Yokoi I
  • Organizer: 37th International Congress of physiological sciences
  • Place of Presentation: Birmingham, UK
• [Presentation] Water-solubleα-lipoic acid derivative, DM-His.Zn, scavenges free radicals, inhibits peroxidation, and protects brain tissue from ischemia-reperfusion injury (2013)
  • Author(s): Tokumaru O, Mizutani Y, Shimada M, Ogata K, Kitano T, Yokoi I
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] Recovery of energy metabolism of rat brain after ischemia-reperfusion injury : a ^<31>P-NMR study (2013)
  • Author(s): Tokumaru O, Kitano T, Yokoi I
  • Organizer: 第90回日本生理学会大会シンポジウム
  • Place of Presentation: 東京
• [Presentation] Neuroprotective effects of a water-solubleα-lipoic acid derivative, DM-His.Zn, on rat brain tissue after ischemia-reperfusion injury : spin resonance analyses study (2013)
  • Author(s): Mizutani Y,Shimada M, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Neuroprotective effects of acetyl-L-carnitine on rat brain slices after ischemia-reperfusion injury : spin resonance analyses study (2013)
  • Author(s): Shimada M, Mizutani Y, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Water-soluble α-lipoic acid derivative, DM-His.Zn, scavenges free radicals, inhibits peroxidation, and protects brain tissue from ischemia-reperfusion injury. (2013)
  • Author(s): Tokumaru O, Mizutani Y, Shimada M, Ogata K, Kitano T, Yokoi I.
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] Neuroprotective effects of acetyl-L-carnitine agonist ischemia-reperfusion injury: a spin resonance study. (2013)
  • Author(s): Tokumaru O, Shimada M, Mizutani Y, Ogata K, Kitano T, Yokoi I.
  • Organizer: 37th International Congress of physiological sciences （IUPS2013，第37回国際生理学会議）
  • Place of Presentation: Birmingham, U.K.
• [Presentation] Recovery of energy metabolism of rat brain after ischemia-reperfusion injury: a 31P-NMR study (2013)
  • Author(s): Tokumaru O, Kitano T, Yokoi I
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Neuroprotective effects of a water-soluble α-lipoic acid derivative, DM-His.Zn, on rat brain tissue after ischemia-reperfusion injury : spin resonance analyses study (2013)
  • Author(s): Mizutani Y,Shimada M, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Neuroprotective effects of acetyl-L-carnitine on rat brain slices after ischemia-reperfusion injury: spin resonance analyses study (2013)
  • Author(s): Shimada M, Mizutani Y, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Water-soluble α-lipoic acid derivative, DM-His.Zn, scavenges free radicals, inhibits peroxidation, and protects brain tissue from ischemia-reperfusion injury (2013)
  • Author(s): Tokumaru O, Mizutani Y,Shimada M, Ogata K, Kitano T, Yokoi I
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] Neuroprotective effects of acetyl-L-carnitine against ischemia-reperfusion injury: a spin resonance study (2013)
  • Author(s): Tokumaru O, Shimada M, Mizutani Y, Ogata K, Kitano T, Yokoi I
  • Organizer: IUPS2013（第37回国際生理学会議）
  • Place of Presentation: Birmingham, U.K.
• [Presentation] Neuroprotective effect of CV159 on brain energy metabolism after ischemia-reperfusion injury : a ^<31>P-NMR study (2012)
  • Author(s): Tokumaru O, Kuroki C, Ogata K, Kitano T, Yokoi I
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Neuroprotective effect of CV159 on brain energy metabolism after ischemia-reperfusion injury: a 31P-NMR study (2012)
  • Author(s): Tokumaru O, Kuroki C, Ogata K, Kitano T, Yokoi I
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Sex-related difference in exponents of Stevens’ power law (2012)
  • Author(s): Tokumaru O, Nakamoto C, Eshima N, Yokoi I
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
• [Presentation] Neuroprotective effects of fructose-1,6-diphosphate on brain energy metabolism after ischemia-reperfusion insult–a ^<31>P-NMR study– (2011)
  • Author(s): Tokumaru O, Kuroki C, Ogata K, Kitano T, Yokoi I
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism
  • Place of Presentation: Barcelona, Spain
• [Presentation] 脳虚血再灌流におけるfructose-1,6-diphosphateの脳保護効果について（31P-NMR実験） (2011)
  • Author(s): 北野敬明，徳丸治，黒木千尋，古賀寛教，野口隆之，横井功
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸
• [Presentation] Neuroprotective effects of fructose-1,6-diphosphate on brain energy metabolism after ischemia-reperfusion insult -a 31P-NMR study- (2011)
  • Author(s): Tokumaru O, Kuroki C, Ogata K, Kitano T, Yokoi I
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism
  • Place of Presentation: Barcelona, Spain
• [Presentation] Neuroprotective effects of hydrogen gas on brain in three types of stress models: a 1H/31P-NMR and ESR study (2011)
  • Author(s): Kuroki C, Tokumaru O, Ogata K, Kitano T, Yokoi I
  • Organizer: XXVth International Symposium on Cerebral Blood Flow, Metabolism
  • Place of Presentation: Barcelona, Spain
• [Presentation] Neuroprotective effect of fructose-1,6-diphosphate on brain energy metabolism after ischemia-reperfusion insult: a 31P-NMR study (2011)
  • Author(s): Tokumaru O, Kuroki C, Kitano T, Yokoi I
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Gamma Knife による機能性脳疾患の治療効果に関する基礎的検討 (2011)
  • Author(s): 徳丸治，黒木千尋，北野敬明，横井功
  • Organizer: 第38回日本脳科学会
  • Place of Presentation: 那覇
• [Presentation] 虚血再灌流負荷に対するフルクトース1,6二リン酸の脳保護作用 (2011)
  • Author(s): 徳丸治，黒木千尋，尾方和枝，北野敬明，横井功
  • Organizer: 第62回西日本生理学会
  • Place of Presentation: 佐賀
• [Presentation] 虚血－再灌流負荷に対するCV159の脳保護作用：31P-NMRによるエネルギー代謝からの検討 (2011)
  • Author(s): 徳丸治，黒木千尋，上林將人，尾方和枝，北野敬明，横井功
  • Organizer: 第32回グアニジノ化合物研究会
  • Place of Presentation: 酒田
• [Presentation] 水溶性αリポ酸誘導体DM-His.Znのラジカル除去作用：ESRによる検討．
  • Author(s): 徳丸治，北野敬明，横井功．
  • Organizer: 第4回癌・炎症とαリポ酸研究会
  • Place of Presentation: 大分・日出
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/anesth/index.html
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/seiri1/