The Inhibitory Effects of Intravenous Anesthetics on Substance P Receptor in U373 MG Human Astrocytoma Cells.
Project/Area Number |
23592265
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Anesthesiology/Resuscitation studies
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Research Institution | Juntendo University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
NAGAOKA Isao 順天堂大学, 医学部, 教授 (60164399)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | サブスタンスP / ケタミン / MAPK / 抗炎症作用 / サイトカイン / サブスタンスP受容体 / 悪性腫瘍 / サブスタンスP受容体 / 麻酔薬 / ペントバルビタール / プロポフォール |
Research Abstract |
The neuropeptide substance P (SP) is an important mediator of neurogenic inflammation within the central and peripheral nervous system. We investigated the effects of ketamine on NF-B phosphorylation, as well as the activation of the mitogen-activated protein kinase (MAPK) in the human astrocytoma cell line U373 MG cells which express high levels of SPR. The level of phosphorylated NF-kB and MAPK (ERK1/2, P-38, and JNK) were detected by western blotting. SP increased the phosphorylation of NF-kB and MAPKs and production of IL-6 and 8. Ketamine attenuated the phosphorylation of NF-kB p65 and MAPKs in U373 MG cells. Production of IL-6 and 8 were suppressed by ketamine. The inhibitory effects of ketamine on SP-induced cytokine production may be mediated by suppressing MAPKs via NF-kB activation. This finding suggests that the inhibition of SPR function by these compounds may be important in the analgesic effects of ketamine.
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Report
(4 results)
Research Products
(15 results)