Analgesic mechanisms of gabapentinoids in spinal cord dorsal horn

• Project/Area Number: 23592277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Niigata University
• Principal Investigator: BABA Hiroshi 新潟大学, 医歯学系, 教授 (00262436)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: プレガバリン / ガバペンチン / 一次求心性線維 / グルタミン酸 / 電位依存性カルシウムチャネル / シナプス前抑制 / 脊髄スライス / カルシウムイメージング / 脊髄後角 / パッチクランプ / 細胞内カルシウムイメージング / 細胞内カルシウム / 単シナプス性 / Caチャネル / 膠様質細胞 / シナプス前終末

Research Abstract

We investigated the effects of gabapentinoids (pregabalin and gabapentin) on glutamatergic synaptic transmission in the spinal cord dorsal horn.Whole cell patch clamp recordings were made from superficial dorsal horn neurons of spinal cord slice with attached dorsal root. Gabapentinoids did not decrease the amplitude of monosynaptic excitatory synaptic currents evoked by dorsal root stimulations. These result suggest that gabapentinoids do not inhibit glutamate release from primary afferent terminals. The inhibition of voltage-gated calcium channels may not be a main analgesic mechanism of these gabapentinoid drugs.

Research Products

• [Journal Article] Effects of ropivacaine concentration on the spread of sensory block produced by continuous thoracic paravertebral block, a prospective, randomised, controlled, double-blind study (2014)
  • Author(s): Yoshida T、Fujiwara T、Furutani K、Ohashi N、Baba H
  • Journal Title: Anaesthesia Volume: 69 Pages: 9-231
  • Peer Reviewed
• [Journal Article] Defining the role of NMDA receptors in anesthesia: Are we there yet? (2014)
  • Author(s): Petrenko AB、Yamakura T、Sakimura K、Baba H
  • Journal Title: Eur J Pharmacol Volume: 723 Pages: 29-37
  • Peer Reviewed
• [Journal Article] Effects of ropivacaine concentration on the spread of sensory block produced by continuous thoracic paravertebral block: a prospective, randomised, controlled, double-blind study (2014)
  • Author(s): Yoshida T, Fujiwara T,Furutani K, Ohashi N, Baba H
  • Journal Title: Anaesthesia Volume: 69 Pages: 231-9
  • Peer Reviewed
• [Journal Article] 神経障害性痛患者に対するプレガバリンの鎮痛効果と副作用-ガバペンチンから変更した症例- (2013)
  • Author(s): 大橋宣子、清水大喜、生駒美穂、岡本学、河野達郎、馬場洋
  • Journal Title: 日本ペインクリニック学会誌 Volume: 20 Pages: 3-111
  • NAID: 10031183206
  • Peer Reviewed
• [Journal Article] Increased brain monoaminergic tone after the NMDA receptor GluN2A subunit gene knockout is responsible for resistance to the hypnotic effect of nitrous oxide (2013)
  • Author(s): Petrenko AB、Yamakura T、Kohno T、Sakimura K、Baba H
  • Journal Title: Eur J Pharmacol Volume: 698 Pages: 5-200
  • Peer Reviewed
• [Journal Article] Increased brain monoaminergic tone after the NMDA receptor GluN2A subunit gene knockout is responsible for resistance to the hypnotic effect of nitrous oxide (2013)
  • Author(s): Petrenko AB, Yamakura T,Kohno T,Sakimura K, Baba H
  • Journal Title: Eur J Pharmacol Volume: 698 Pages: 200-5
  • Peer Reviewed
• [Presentation] 脊髄スライスを用いて行ってきた基礎研究 (2014)
  • Author(s): 馬場洋
  • Organizer: 第30回愛媛麻酔科集談会総会
  • Place of Presentation: 松山市
  • Year and Date: 2014-03-08
• [Presentation] Effects of Ropi- vacaine Concentration on the Spread of Sensory Block Produced by Con- tinuous Paravertebral Block (2013)
  • Author(s): Yoshida T、Fujiwara T、Furutani K、Ohashi N、Baba H
  • Organizer: American Society of Anesthesiologists 2013 Annual Meeting、、San Francisco
  • Place of Presentation: USA
  • Year and Date: 2013-10-13
• [Presentation] 血流遮断によるしびれの脊髄メカニズム (2013)
  • Author(s): 渡部達範、駒形成司、塚野浩明、菱田竜一、河野達郎、馬場洋、澁木克栄
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
  • Year and Date: 2013-06-22
• [Presentation] 臨床濃度のプレガバリン・ガバペンチンは糖尿病性神経障害性痛動物モデルにおいて一次求心性終末からのグルタミン酸の放出を抑制しない (2013)
  • Author(s): 馬場洋、高松美砂子、生駒美穂、渡部達範
  • Organizer: 日本麻酔科学会第60回学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2013-05-23
• [Presentation] DHEASの脊髄後角における痛覚伝達への作用 (2013)
  • Author(s): 山本豪、生駒美穂、紙谷義孝、河野達郎、馬場洋
  • Organizer: 第35回脊髄機能診断研究会
  • Place of Presentation: 東京
  • Year and Date: 2013-02-01
• [Presentation] 臨床濃度のプレガバリン・ガバペンチンは糖尿病性神経障害性痛動物モデルにおいて、一次求心性終末からのグルタミン酸の放出を抑制しない (2013)
  • Author(s): 馬場 洋、髙松美砂子、生駒美穂、渡辺達範
  • Organizer: 第60回日本麻酔科学会
  • Place of Presentation: 札幌
• [Presentation] 臨床濃度のプレガバリン・ガバペンチンは糖尿病性神経障害性痛動物モデルにおいて、一次求心性終末からのグルタミン酸の放出を抑制しない
  • Author(s): 馬場洋、高松美砂子、生駒美穂、渡部達範
  • Organizer: 日本麻酔科学会第60回学術集会
  • Place of Presentation: 札幌
• [Presentation] Effects of Ropivacaine Concentration on the Spread of Sensory Block Produced by Continuous Paravertebral Block
  • Author(s): Yoshida T, Fujiwara T, Furutani K, Ohashi N, Baba H
  • Organizer: American Society of Anesthesiologists 2013 Annual Meeting
  • Place of Presentation: San Francisco
• [Remarks]
  • URL: http://www.med.niigata-u.ac.jp/ane/