Insulin preconditioning elevates p-Akt and cardiac contractility after reperfusion in the isolated ischemic rat heart

• Project/Area Number: 23592280
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: University of Yamanashi
• Principal Investigator: SATO Hiroaki 山梨大学, 医学部附属病院, 助教 (20402026)
• Co-Investigator(Kenkyū-buntansha): OGUCHI Takeshi 山梨大学, 医学工学総合研究部, 准教授 (60201399) ; MATSUKAWA Takashi 山梨大学, 医学工学総合研究部, 教授 (80209519)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: インスリン / 虚血再灌流障害 / 心筋保護 / Insulin / preconditioning effect / rat heart / ischemia

Research Abstract

The optimal timing of insulin institution related to cardiac ischemia was unclear.We tested the hypothesis that insulin administered prior to ischemia provides better cardioprotection than insulin administration after ischemia.
Hearts isolated from male rats were prepared using Langendorff method and divided into three groups.
In this study, we concluded insulin administration prior to ischemia provides better cardioprotection than insulin administration only at reperfusion in the isolated ischemic rat heart.

Research Products

• [Presentation] 心臓虚血再灌流モデルにおける高用量インスリン先行投与の心筋保護効果 (2013)
  • Author(s): 福島久史、他
  • Organizer: 第60回日本麻酔科学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-05-23
• [Presentation] 心臓虚血再灌流モデルにおける高用量インスリン先行投与の心筋保護効果 (2013)
  • Author(s): 福島久史、佐藤宏明、佐藤環、小口健史、中楯陽介、松川隆
  • Organizer: 日本麻酔科学会第60回学術集会
  • Place of Presentation: ロイトン札幌（北海道札幌市）
• [Presentation] Preconditioning Effect of High Dose Insulin in Isolated Rat Heart. American Society of Anesthesiologists (ASA) (2012)
  • Author(s): 福島久史、他
  • Organizer: 2012 Annual Meeting
  • Place of Presentation: Washington,DC
  • Year and Date: 2012-10-16
• [Presentation] Preconditioning Effect of High Dose Insulin in Isolated Rat Heart (2012)
  • Author(s): Hisashi Fukushima
  • Organizer: ASA2012
  • Place of Presentation: ワシントン、USA
• [Presentation] Effect of Hyperinsulinemic Normoglycemic Clamp on Isolated Rat Heart. American Society of Anesthesiologists (ASA) (2011)
  • Author(s): 佐藤環、他
  • Organizer: 2011 Annual Meeting
  • Place of Presentation: Chicago,Illinois
  • Year and Date: 2011-10-18
• [Presentation] Effect of Hyperinsulinemic Normoglycemic Clamp on Isolated Rat Heart (2011)
  • Author(s): Tamaki Sato
  • Organizer: Anesthesiology 2011 (ASA annual meeting)
  • Place of Presentation: McCormick Place, Chicago, USA
• [Remarks] 山梨大学医学部麻酔科学講座心筋代謝実験 グループ
  • URL: http://y-anesth.jp/research/shinkin/
• [Remarks] 山梨大学麻酔科学教室心筋代謝研究室
  • URL: http://y-anesth.jp/research/shinkin/
• [Remarks] 山梨大学附属病院麻酔科HP
  • URL: http://y-anesth.jp/