Analysis of the molecular mechanism of the interaction of heterodimerized m-opioid and CB1 receptors
| Project/Area Number |
23592299
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Nagasaki University |
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Principal Investigator |
HOJO Minoru 長崎大学, 医歯薬学総合研究科(医学系), 助教 (00380957)
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| Co-Investigator(Kenkyū-buntansha) |
UEZONO Yasuhito 国立がん研究センター, 研究所, 分野長 (20213340)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | オピオイド / カナビノイド / 鎮痛 / 二量体 / 疼痛 / 痛み |
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| Outline of Final Research Achievements |
The marijuana is known as a drug producing for a long time. Cannabinoid CB1 which is the chief ingredient of the marijuana is used in multiple sclerosis and AIDs, cancer patients even now. To visually and biochemically demonstrate the heterodimerization of mOR and CB1R, we carried out confocal microscopy and fluorescence resonance energy transfer (FRET), and immunoprecipitation
followed by Western blot analysis. We also examined signal transduction pathways activated by dimerized mOR/CB1R with the electrophysiological assay of G protein-activated inwardly rectifying K+ (GIRK) channels and the measurement of total and phosphorylated extracellular signal-regulated kinases (ERK).
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] GABA_B receptors do not internalize after baclofen treatment possibly due to lack of theβ-arrestin association ; a real-time visualization study2012
Author(s)
Sudo, Y., Hojo, M., Ando, Y., Takada, M., Murata, H., Kurata, S., Kanaide, M., Nishida, N., Uezono, Y.
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Journal Title
Synapse, Epub ahead of print
Volume: 66 (9)
Issue: 9
Pages: 759-769
DOI
Related Report
Peer Reviewed
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[Journal Article] OPTIMプロジェクト前後での病院から在宅診療への移行率と病院医師・看護師の在宅の視点の変化2012
Author(s)
白髭 豊, 野田 剛稔, 北條 美能留, 後藤 慎一, 冨安 志郎, 出口 雅浩, 奥平 定之, 安中 正和, 平山 美香, 吉原 律子, 船本 太栄子, 五十嵐 歩, 宮下 光令, 森田 達也
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Journal Title
Palliative Care Research
Volume: 7巻2号
Pages: 389-394
NAID
Related Report
Peer Reviewed
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[Journal Article] Possible involvement of prolonging spinalμ-opioid receptor desensitization in the development of antihyperalgesic tolerance toμ-opioids under a neuropathic pain-like state2011
Author(s)
Narita, M., Imai, S., Nakamura, A., Ozeki, A., Asato, M., Rahmadi, M., Sudo, Y., Hojo, M., Uezono, Y., Devi, L. A., Kuzumaki, N., Suzuki, T.
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Journal Title
Addict Biol
Volume: Aug 4
Issue: 4
Pages: 1369-1600
DOI
Related Report
Peer Reviewed
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