Analysis of pain hypersensitivity due to the mTOR mediated proteome alteration in the primary afferent neurons.
| Project/Area Number |
23592306
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
AMAYA Fumimasa 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60347466)
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| Co-Investigator(Kenkyū-buntansha) |
SAWA Teiji 京都府立医科大学, 医学研究科, 教授 (10206013)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 痛覚過敏 / mTOR / IGF / 一次知覚神経 / 組織損傷 / VGLUT2 |
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| Outline of Final Research Achievements |
Phosphorylated mTOR (pmTOR) expression was detected in the primary afferent neurons in the dorsal root ganglion (DRG). Expression of pmTOR increased after plantar incision. mTOR inhibitor rapamycin reduced plantar incision-induced hyperalgesia. Intralpantar injection of IGF increased pmTOR expression and IGF inhibitor inhibited plantar incision-induced pmTOR induction. VGLUT3 expression was increased in the DRG after the plantar incision and rapamycin prevented incision-induced VGLUT-3 increase. IGF injection into the paw increased VGLUT-3 expression in the DRG.
These results demonstrated that increased local production of IGF facilitates mTOR phosphorylation and increase VGLUT3 expression in the DRG neurons, leading pain hypersensitivity after the tissue injury.
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Report
(5 results)
Research Products
(11 results)
