New treatment strategy of bladder cancer indexed by centrosome amplification
| Project/Area Number |
23592333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Kohsuke 山口大学, 大学院医学系研究科, 教授 (80116722)
HARA Takahiko 山口大学, 医学部附属病院, 助教 (70511715)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 膀胱癌 / 中心体複製異常 / 薬剤感受性 / パクリタキセル |
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| Research Abstract |
The aims of this study were to identify the relevant gene of centrosome amplification (CA) and its regulating microRNA (miR) in bladder cancer, to establish the CA-positive from CA-negative (KK-47) bladder cancer cell line by the gene transfer technique, and to compare the invasive phenotype, or sensitivity of paclitaxel between parent and gene-transferred cell line. To pick-up the relevant genes to CA, array CGH technique was applied and referred the open miR database to find the relevant miR regulating such genes. As a result, hsa-miR-1225-5p and GBAS was selected as miR and relevant gene to control CA, respectively. The GBAS transferred KK-47 cell lined showed significantly higher cell population with CA than parent KK-47 cell lines. Comparison of invasive phenotype, sensitivity of paclitaxel between parent and gene-transferred cell line are under investigation.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Copy number aberrations using multicolour fluorescence in situ hybridization (FISH) for prognostication in non-muscle-invasive bladder cancer (NIMBC)2014
Author(s)
Matsuyama H, Ikemoto K, Eguchi S, Oga A, Kawauchi S, Yamamoto Y, Kawai Y, Matsumoto H, Hara T, Nagao K, Sakano S, Sasaki K
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Journal Title
BJU Int
Volume: 113
Pages: 7-662
Related Report
Peer Reviewed
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[Journal Article] Copy number aberrations using multicolour fluorescence in situ hybridization (FISH) for prognostication in non-muscle-invasive bladder cancer (NIMBC).2014
Author(s)
Matsuyama H, Ikemoto K, Eguchi S, Oga A, Kawauchi S, Yamamoto Y, Kawai Y, Matsumoto H, Hara T, Nagao K, Sakano S, Sasaki K.
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Journal Title
BJU Int.
Volume: 113
Issue: 4
Pages: 662-667
DOI
Related Report
Peer Reviewed
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[Journal Article] DNA copy number aberrations associated with Lymphovascular invasion in upper urinary tract urothelial carcinoma2012
Author(s)
Misumi, T, Yamamoto Y, Miyachika Y, Eguchi S, Chochi Y, Nakao M, Nagao K, Hara T, Sakano S, Furuya T, Oga A, Kawauchi S, Sasaki K, Matsuyama H
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Journal Title
Cancer Genet
Volume: 205
Pages: 8-313
Related Report
Peer Reviewed
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[Journal Article] Centrosome amplification as a putative prognostic biomarker for the classification of urothelial carcinomas2011
Author(s)
Yamamoto Y, Misumi T, Eguchi S, Chochi Y, Kitahara S, Nakao M, Nagao K, Hara T, Sakano S, Furuya T, Oga A, Kawauchi S, Sasaki K, Matsuyama H
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Journal Title
Hum Pathol
Volume: 42
Pages: 30-1923
Related Report
Peer Reviewed
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[Presentation] Centrosome amplification in bladder washing cytology samples may become prognostic marker in non-muscle invasive bladder cancer2012
Author(s)
Miyachika, Y., Yamamoto, Y., Matsumoto, H., Nishijima, J., Kawai, Y., Hara, T., Sakano, S., Matsuyama, H
Organizer
AUA annual meeting
Place of Presentation
Atlanta,USA
Year and Date
2012-05-22
Related Report
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[Presentation] Centrosome amplification in bladder washing cytology samples may become prognostic marker in non-muscle invasive bladder cancer.2012
Author(s)
Miyachika, Y., Yamamoto, Y., Matsumoto, H., Nishijima, J., Kawai, Y., Hara, T., Sakano, S., Matsuyama, H.
Organizer
AUA (American Urological Association) annual meeting.
Place of Presentation
Atlanta, USA
Related Report
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