| Project/Area Number |
23592333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Kohsuke 山口大学, 大学院医学系研究科, 教授 (80116722)
HARA Takahiko 山口大学, 医学部附属病院, 助教 (70511715)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 膀胱癌 / 中心体複製異常 / 薬剤感受性 / パクリタキセル |
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| Research Abstract |
The aims of this study were to identify the relevant gene of centrosome amplification (CA) and its regulating microRNA (miR) in bladder cancer, to establish the CA-positive from CA-negative (KK-47) bladder cancer cell line by the gene transfer technique, and to compare the invasive phenotype, or sensitivity of paclitaxel between parent and gene-transferred cell line. To pick-up the relevant genes to CA, array CGH technique was applied and referred the open miR database to find the relevant miR regulating such genes. As a result, hsa-miR-1225-5p and GBAS was selected as miR and relevant gene to control CA, respectively. The GBAS transferred KK-47 cell lined showed significantly higher cell population with CA than parent KK-47 cell lines. Comparison of invasive phenotype, sensitivity of paclitaxel between parent and gene-transferred cell line are under investigation.
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