| Project/Area Number |
23592335
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kochi University |
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Principal Investigator |
INOUE Keiji 高知大学, 教育研究部医療学系, 准教授 (00294827)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Kenji 高知大学, 教育研究部医療学系, 助教 (50464384)
SHUIN Taro 高知大学, 教育研究部医療学系, 教授 (70128601)
OGURA Shunichiro 東京工業大学, 生命理工学研究科, 准教授 (90343160)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 膀胱癌 / 光力学診断 / アミノレブリン酸 / 偽陽性病変 / 遺伝子解析 |
|---|
| Research Abstract |
Comprehensive gene analysis of false positives in aminolevulinic acid-induced photodynamic diagnosis were performed using biopsy specimen of urinary bladder cancer in this study. As a result of measuring the level of mRNA obtained from positive fluorescent cancerous lesion and negative fluorescent non-cancerous lesion, high expression of peptide transporter PEPT1 and low expression of human ATP-binding cassette trans-porter ABCG2 were demonstrated the responsible genes for cancer-specific overaccumulation. Moreover, as a result of mass analysis by high performance liquid chromatography, the precursors of PpIX, uroporphyrin and coproporphyrin, were overexcreted to urine by the cancer-specific abnormal activity of these PpIX-related transporters.
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