Comprehensive gene analysis of false positive urothelium in photodynamic diagnosis to explore the novel carcinogenesis-related gene
| Project/Area Number |
23592335
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kochi University |
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Principal Investigator |
INOUE Keiji 高知大学, 教育研究部医療学系, 准教授 (00294827)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Kenji 高知大学, 教育研究部医療学系, 助教 (50464384)
SHUIN Taro 高知大学, 教育研究部医療学系, 教授 (70128601)
OGURA Shunichiro 東京工業大学, 生命理工学研究科, 准教授 (90343160)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 膀胱癌 / 光力学診断 / アミノレブリン酸 / 偽陽性病変 / 遺伝子解析 |
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| Research Abstract |
Comprehensive gene analysis of false positives in aminolevulinic acid-induced photodynamic diagnosis were performed using biopsy specimen of urinary bladder cancer in this study. As a result of measuring the level of mRNA obtained from positive fluorescent cancerous lesion and negative fluorescent non-cancerous lesion, high expression of peptide transporter PEPT1 and low expression of human ATP-binding cassette trans-porter ABCG2 were demonstrated the responsible genes for cancer-specific overaccumulation. Moreover, as a result of mass analysis by high performance liquid chromatography, the precursors of PpIX, uroporphyrin and coproporphyrin, were overexcreted to urine by the cancer-specific abnormal activity of these PpIX-related transporters.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Expression levels of PEPT1 and ABCG2 play key roles in 5-aminolevulinic acid (ALA)-induced tumor-specific protoporphyrin IX (PpIX) accumulation in bladder cancer.2013
Author(s)
Yuichiro Hagiya, Hideo Fukuhara, Kentaro Matsumoto, Yoshio Endo, Motowo Nakajima, Tohru Tanaka, Ichiro Okura, Atsushi Kurabayashi, Mutsuo Furihata, Keiji Inoue, Taro Shuin, Shun-ichiro Ogura
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Journal Title
Photodiagnosis and Photodynamic Therapy
Volume: 印刷中
Issue: 3
Pages: 288-295
DOI
Related Report
Peer Reviewed
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[Journal Article] 膀胱癌診療における光力学診断(PDD)およびスクリーニング(PDS)の有用性、シンポジウムIII「膀胱癌の再発予防について」2013
Author(s)
井上啓史,福原秀雄,川田千明,執印太郎,倉林睦,降幡睦夫,小倉俊一郎,石塚昌宏,高橋究,太田麗,石井琢也,田中徹
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Journal Title
日本腎泌尿器疾患予防医学研究会雑誌
Volume: 21(1)
Pages: 48-51
Related Report
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[Journal Article] 膀胱癌診療における光力学診断（PDD）およびスクリーニング（PDS）の有用性 シンポジウムIII 「膀胱癌の再発予防について」2013
Author(s)
井上啓史, 福原秀雄, 川田千明, 執印太郎, 倉林 睦, 降幡睦夫, 小倉俊一郎, 石塚昌宏, 高橋 究, 太田 麗, 石井琢也, 田中 徹
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Journal Title
日本腎泌尿器疾患予防医学研究会雑誌
Volume: 21
Pages: 48-51
Related Report
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