| Project/Area Number |
23592338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kumamoto University |
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Principal Investigator |
WADA Yoshihiro 熊本大学, 大学院生命科学研究部, 准教授 (20284755)
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Takahisa 熊本大学, 大学院生命科学研究部, 准教授 (20176499)
KAWANO Yoshiaki 熊本大学, 大学院生命科学研究部, 助教 (30593793)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 前立腺癌 / 去勢不応答 / プロテアーゼ / 細胞膜 / ﾎﾙﾓﾝ不応性前立腺癌 / 基質 / 増殖・浸潤・転移 / プロテオミクス解析 |
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| Research Abstract |
Protease activities in culture media of prostate cancer cells were measured using 95 dipeptidyl substrates and a hydrolytic activity for Ac-XZ-MCA was found in media of castration-resistant but not -sensitive type cells. This activity was completely inhibited by DFP but not by other inhibitors, indicating this protease is serine-type. This activity was much higher in the washed cell suspensions than the culture supernatants and was localized on the cancer cell membrane when stained using biotin-XZ-chloromethylketone, which revealed that this protease is not secreted to the media but bound to the cell membrane. This protease was separated from the cell membrane and subjected to proteomics analysis but has not been identified because of a small amount of the protease. This castration-resistant prostate cancer cell-specific protease is a promising therapeutic target, thus further purification procedures will be done for its identification.
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