| Project/Area Number |
23592344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
AKITA Hidetoshi 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (10381782)
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| Co-Investigator(Kenkyū-buntansha) |
TOZAWA Keiichi 名古屋市立大学, 大学院医学研究科, 准教授 (40264733)
HASHIMOTO Yoshihiro 名古屋市立大学, 大学院医学研究科, 研究員 (40244561)
KOHRI Kenjiro 名古屋市立大学, 大学院医学研究科, 教授 (30122047)
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| Research Collaborator |
YAKADA Kenji 名古屋市立大学, 大学院医学研究科, 研究員 (80566232)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 膀胱がん / UQCRB / コピー数 / ＵＱＣＲＢ / copy number aberration |
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| Research Abstract |
In this study, to investigate chromosomal instabilities (CIN), bladder cancer mouse model was used. As measure of CIN, DNA copy number aberrations (CNA) were investigated. According to array comparative genomic hybridization (CGH) analysis to detect CNA throughout the genome, five chromosomes with high CIN were identified, and among the candidate genes present in these regions, ubiquinol-cytochrome c reductase binding protein (UQCRB) on chromosome13B3 was further characterized. In this study, both CNA and protein expression of UQCRB in mouse model were examined. Both CNA and protein expression of UQCRB of tumor at early stages were significantly increased than control tissue and invasive cancer. Quantitative PCR and immunohistochemical analyses indicated that the CNA and the protein expression of UQCRB at low T stage were higher than those at high T stages in human bladder cancer specimens. These findings suggest that UQCRB is an indicator of bladder cancer.
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