Early diagnosis of prostate cancer using cancer-specific the glycosylated peptide antibody

• Project/Area Number: 23592350
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Juntendo University
• Principal Investigator: FUJIMURA Tsutomu 順天堂大学, 医学(系)研究科(研究院), 准教授 (70245778)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 悪性前立腺がん / ハプトグロビン / 糖鎖修飾ペプチド / ペプチド抗体 / がん特異的抗体 / シアル酸化 / フコシル化 / ELISA / シアル酸 / ＥＬＩＳＡ法 / PSA値 / 国際情報交流

Research Abstract

[Purpose] I found haptoglobin who received sugar chain modification cancer-specific from malignant prostate cancer patients serum. The antibody was produced to antigen glycosylated peptide from a cancer patient serum-derived haptoglobin. I aimed at the early diagnosis of prostate cancer. [Result] Antibody produced was possible to distinguish healthy control and malignant prostate cancer patients. To also distinguish between malignant prostate cancer and benign disease patients was also possible. The prototype that can be measured by ELISA was able to sufficient sensitivity is obtained.It was detectable the number ng / ml sugar chain haptoglobin in serum. I was able to produce ELISA to make up for the shortcomings of PSA levels. Furthermore, we want to raise the accuracy of the early diagnosis method by increase the number of samples.

Research Products

• [Journal Article] Sialyl-glycoconjugates in cholesterol-rich microdomains of P388 cells are the triggers for apoptosis induced by Rana catesbeiana oocyte ribonuclease. (2014)
  • Author(s): Ogawa Y, Fujimura T, Ozeki Y, et al.
  • Journal Title: Glycoconj J. Volume: 31 Issue: 2 Pages: 171-84
  • DOI: 10.1007/s10719-013-9513-7
  • Peer Reviewed
• [Journal Article] Role of the TNF pathway in the progression of diabetic nephropathy in KK-Ay mice. (2014)
  • Author(s): Omote K, Fujimura T, Tomino Y, et al.
  • Journal Title: Am J Physiol Renal Physiol. Volume: in press
  • Peer Reviewed
• [Journal Article] PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. (2014)
  • Author(s): Furuya N, Ikeda SI, Sato S, Soma S, Ezaki J, Trejo JA, Takeda-Ezaki M, Fujimura T, Arikawa-Hirasawa E, Tada N, Komatsu M, Tanaka K, Kominami E, Ueno T.
  • Journal Title: Autophagy Volume: 10 Issue: 4 Pages: 631-41
  • DOI: 10.4161/auto.27785
  • Peer Reviewed
• [Journal Article] Galectin-4, a novel predictor for lymph node metastasis in lung adenocarcinoma. (2013)
  • Author(s): Hayashi T, Fujimura T, Yao T, et al.
  • Journal Title: PLoS One Volume: 8 Issue: 12 Pages: e81883-e81883
  • DOI: 10.1371/journal.pone.0081883
  • Peer Reviewed
• [Journal Article] Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus. (2013)
  • Author(s): Ouchi T, Fujimura T, et al.
  • Journal Title: Nat Chem Biol. Volume: 9(4):277-83 Issue: 4 Pages: 277-283
  • DOI: 10.1038/nchembio.1200
  • Peer Reviewed
• [Journal Article] NP-1250, an ABCG2 inhibitor, induces apoptotic cell death in breast carcinoma MCF7/mitoxantrone-resistant cells via a caspase-independent pathway. (2013)
  • Author(s): Masumi Ito
  • Journal Title: Oncology Reports Volume: 29 (4) Issue: 4 Pages: 1495-1500
  • DOI: 10.3892/or.2013.2249
  • Peer Reviewed
• [Journal Article] Molecular interaction of the first 3 enzymes of the de novo pyrimidine biosynthetic pathway of Trypanosoma cruzi (2012)
  • Author(s): Takeshi Nara
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 418 Issue: 1 Pages: 140-143
  • DOI: 10.1016/j.bbrc.2011.12.148
  • Peer Reviewed
• [Journal Article] Multi-sequential SPR-analysis of haptoglobin-lectin complex in sera of patients with malignant and benign prostate diseases (2011)
  • Author(s): Kazuno S, Fujimura T, Arai T, Ueno T, Nagao K, Fujime M, Murayama K
  • Journal Title: Anal Biochem Volume: 419 Issue: 2 Pages: 241-249
  • DOI: 10.1016/j.ab.2011.08.029
  • Peer Reviewed
• [Journal Article] Liver autophagy contributes to the maintenance of blood glucose and amino acid levels (2011)
  • Author(s): Ezaki J, Komatsu M, Takahashi K, Fujimura T, Tanaka K, Kominami E, Ueno T, et al
  • Journal Title: Autophagy Volume: 7 Pages: 727-736
• [Journal Article] Liver autophagy contributes to the maintenance of blood glucose and amino acid levels. (2011)
  • Author(s): Ezaki J., et al.
  • Journal Title: Autophagy Volume: 7巻 Pages: 727-736
  • Peer Reviewed
• [Presentation] Proteomic Approaches To Define The Functional Role Of Nucleophosmin In Ewing's Sarcomas. (2014)
  • Author(s): Suehara Y, Kohsaka S, Shukla N, Kubota D, Mineki R, Fujimura T, Kaneko K, Saito T, Ladanyi M.
  • Organizer: Orthopaedic Research Society 60th Annual Meeting
  • Place of Presentation: New Orleans in USA
• [Presentation] Metabolic shift in hypoxia-adapted CML cells and mechanisms of acquired dasatinib resistance uncovered by proteomic iTRAQ profiling (2013)
  • Organizer: 第55回アメリカ血液学会
  • Place of Presentation: 米国
• [Presentation] 呼気中のがんマーカーの探索 (2013)
  • Organizer: 第1回がんと代謝研究会
  • Place of Presentation: 山形県鶴岡市
• [Presentation] Search for new urothelial cancer biomarkers (2013)
  • Organizer: 12^<th>、HUPO World Congress
  • Place of Presentation: パシフィコ横浜
• [Presentation] Oligosaccharide structure analysis of Acute Phase Protein in the serum of the prostate cancer patient (2013)
  • Organizer: 12^<th>、HUPO World Congress
  • Place of Presentation: パシフィコ横浜
• [Presentation] 前立腺がん患者血清中のIgGの糖鎖構造解析 (2013)
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Oligosaccharide structure analysis of IgG in the serum of the prostate cancer patient. (2013)
  • Author(s): Kazuno S, Murayama K, Ueno T, Furukawa J, Shinohara Y, Fujimura T.
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] Oligosaccharide structure analysis of Acute Phase Protein in the serum of the prostate cancer patient. (2013)
  • Author(s): Fujimura T, Kazuno S, Murayama K, Ueno T, Furukawa J, Shinohara Y.
  • Organizer: The 12th HUPO World Congress
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] Search for new urothelial cancer biomarkers. (2013)
  • Author(s): Fujimura T, Taka H, Kazuno S, Mineki R, Murayama K, Ueno T, Furukawa J, Shinohara Y.
  • Organizer: The 12th HUPO World Congress
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Presentation] 呼気中のがんマーカーの探索 (2013)
  • Author(s): 藤村務、加賀直子、松森聖、梶山美明、高ひかり、上野隆
  • Organizer: 第1回がんと代謝研究会
  • Place of Presentation: 山形県・鶴岡市
• [Presentation] Metabolic shift in hypoxia-adapted CML cells and mechanisms of acquired dasatinib resistance uncovered by proteomic iTRAQ profiling. (2013)
  • Author(s): Tabe Y, Jin L, Iwanami H, Matsushita H, Kazuno S, Fujimura T, Ueno T, Miida T.
  • Organizer: 55th American Society of Hematology Annual Meeting
  • Place of Presentation: New Orleans in U.S.A.
• [Presentation] Integrative genomic and proteomic analysis of low-dose ionizing irradiation effects on bone marrow stromal microenvironment and on survival of pre-leukemic cells. (2013)
  • Author(s): Tabe Y, Jin L, Hatanaka Y, Matsushita H, Kazuno S, Fujimura T, Ueno U, Sasai K, Iwabuchi K, Andreeff M, Konopleva M, MD, Miida T.
  • Organizer: 55th American Society of Hematology Annual Meeting
  • Place of Presentation: New Orleans in U.S.A.
• [Presentation] Efficacy and mechanisms of the mTOR inhibitor AZD2014 combined with L-Asparaginase or JAK2 inhibitor TG101348 in ALL. (2013)
  • Author(s): Tabe Y, Jin L, Iwanami H, Matsushita H, Kazuno S, Fujimura T, Ueno T, Miida T, Weinstock D, Thomas D.A, Andreeff M, Konopleva M.
  • Organizer: 55th American Society of Hematology Annual Meeting
  • Place of Presentation: New Orleans in USA
• [Presentation] Molecular mechanisms of adipocyte-leukemia interactions revealed by the quantitative proteomics technology: pro-survival function of adipocyte-derived free fatty acids on acute monoblastic leukemia mitochondrial biogenesis. (2013)
  • Author(s): Tabe Y, Jin L, Kazuno S, Fujimura T, Matsushita H, Ueno T, Miida T, Andreeff M, Konopleva M.
  • Organizer: 55th American Society of Hematology Annual Meeting
  • Place of Presentation: New Orleans in USA
• [Presentation] Molecular mechanisms of inhibition of ribosomal biogenesis and translational flux by the Selective Inhibitor of Nuclear Export (SINE) XPO1/CRM1 antagonist KPT-185 in mantle cell lymphoma. (2013)
  • Author(s): Tabe Y, Kojima K, Jin L, Iwanami H, Matsushita H, Kazuno S, Kauffman M, Shacham S, Fujimura T, Ueno U, Miida T, Andreeff M.
  • Organizer: 55th American Society of Hematology Annual Meeting
  • Place of Presentation: New Orleans in USA
• [Presentation] Analysis of Dasatinib-Induced Molecular Mechanisms of Apoptosis in Hypoxia-Adopted CML Cells Utilizing Quantitative Proteomics Technology (2012)
  • Organizer: 第54回アメリカ血液学会
  • Place of Presentation: 米国
• [Presentation] 白血病細胞のメタボロミクス解析 (2012)
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県福岡市
• [Presentation] 尿路上皮がんにおける新規バイオマーカーの探索 (2012)
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県福岡市
• [Presentation] リンパ系腫瘍細胞のメタボロミクス解析 (2012)
  • Organizer: 第7回メタボロームシンポジウム
  • Place of Presentation: 山形県鶴岡市
• [Presentation] リンパ系腫瘍細胞のメタボロミクス解析 (2012)
  • Author(s): 藤村 務
  • Organizer: 第7回メタボロームシンポジウムに
  • Place of Presentation: 山形県鶴岡市
• [Presentation] 白血病細胞のメタボロミクス解析 (2012)
  • Author(s): 藤村 務
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県福岡市博多区
• [Presentation] 尿路上皮がんにおける新規バイオマーカーの探索 (2012)
  • Author(s): 高 ひかり、藤村 務
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県福岡市博多区
• [Presentation] 前立腺癌患者血清に含まれるAcute Phase Proteinの糖鎖構造解析 (2011)
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Carbohydrate chain structure analysis of Acute Phase Protein included in prostate cancer patient serum. (2011)
  • Author(s): 藤村 務
  • Organizer: 第８４回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
• [Book] 臓病実験法ハンドブック (2013)
  • Author(s): 富野康日己、藤村務, 他
  • Total Pages: 168
  • Publisher: 中外医学社
• [Book] 腎臓病実験法ハンドブック (2013)
  • Author(s): 富野康日己（分担：藤村 務）
  • Total Pages: 238
  • Publisher: 中外医学社
• [Book] 腎臓病実験法ハンドブック (2013)
  • Author(s): 富野康日己 （分担：藤村 務）
  • Total Pages: 350
  • Publisher: 中外医学社
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/seitai_bunshi/k3.html