Development of new therapy using novel chimeric oncolytic adenoviruse vector for intractable bladder cancer.

• Project/Area Number: 23592354
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Hyogo Medical University
• Principal Investigator: GOTOH Akinobu 兵庫医科大学, 医学部, 教授 (70283885)
• Co-Investigator(Kenkyū-buntansha): NAGAYA Hisao 兵庫医科大学, 医学部, 助教 (60464343)
• Co-Investigator(Renkei-kenkyūsha): TAGAWA Masatoshi 千葉県がんセンター, 病理研究部, 部長 (20171572)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膀胱癌 / アデノウイルスベクター / 遺伝子治療 / CD46 / CAR / 腎臓癌 / アデノウイルス / CD46蛋白質 / 抗腫瘍効果

Research Abstract

In this study, we examined whether the fiber knob substituted adenovirus vector enhance the therapeutic effect of gene therapy for human bladder cancer which do not have a significant effect on therapeutic outcome of adenovirus gene therapy. Our data showed the novel fiber knob substituted adenovirus vector, Ad5F35/MK, is more useful for the gene therapy of bladder cancer than the non fiber knob substituted Ad5/MK is for human bladder cancer in vitro and in vivo.

Research Products

• [Journal Article] Crosstalk between PI3 Kinase/PDK1/Akt/Rac1 and Ras/Raf/MEK/ERK Pathways Downstream PDGF Receptor (2013)
  • Author(s): Niba ET, Nagaya H, Kanno T, Tsuchiya A, Gotoh A, Tabata C, Kuribayashi K, Nakano T, Nishizaki T
  • Journal Title: Cell Physiol Biochem Volume: 31 Pages: 905-13
  • Peer Reviewed
• [Journal Article] Naftopidil induces apoptosis in malignant mesothelioma cell lines independently ofα1-adrenoceptor blocking (2013)
  • Author(s): Masachika E, Kanno T, Nakano T, Gotoh A, Nishizaki T
  • Journal Title: Anticancer Res Volume: 33 Pages: 887-94
  • Peer Reviewed
• [Journal Article] Fiber-substituted conditionally replicating adenovirus Ad5F35 induces oncolysis of human bladder cancer cells in in vitro analysis (2013)
  • Author(s): Gotoh A, Nagaya H, Kanno T, Tagawa M, Nishizaki T
  • Journal Title: Urology Volume: 81
  • Peer Reviewed
• [Journal Article] A_3 Adenosine receptor mediates apoptosis in in vitro RCC4-VHL human renal cancer cells by up-regulating AMID expression (2013)
  • Author(s): Nagaya H, Gotoh A, Kanno T, Nishizaki T
  • Journal Title: J Urol Volume: 189 Pages: 321-8
  • Peer Reviewed
• [Journal Article] Crosstalk between PI3 Kinase/PDK1/Akt/Rac1 and Ras/Raf/MEK/ERK Pathways Downstream PDGF Receptor. (2013)
  • Author(s): Niba ET, Nagaya H, Kanno T, Tsuchiya A, Gotoh A, Tabata C, Kuribayashi K, Nakano T, Nishizaki T
  • Journal Title: Cell Physiol Biochem Volume: 31 Issue: 6 Pages: 905-913
  • DOI: 10.1159/000350108
  • Peer Reviewed
• [Journal Article] Naftopidil induces apoptosis in malignant mesothelioma cell lines independently of α1-adrenoceptor blocking. (2013)
  • Author(s): Masachika E, Kanno T, Nakano T, Gotoh A, Nishizaki T
  • Journal Title: Anticancer Res Volume: 33 Pages: 887-894
  • Peer Reviewed
• [Journal Article] Fiber-substituted conditionally replicating adenovirus Ad5F35 induces oncolysis of human bladder cancer cells in in vitro analysis. (2013)
  • Author(s): Gotoh A, Nagaya H, Kanno T, Tagawa M, Nishizaki T.
  • Journal Title: Urology. Volume: 81 Issue: 4 Pages: 920.e7-920.e11
  • DOI: 10.1016/j.urology.2012.12.023
  • Peer Reviewed
• [Journal Article] A3 Adenosine receptor mediates apoptosis in in vitro RCC4-VHL human renal cancer cells by up-regulating AMID expression. (2013)
  • Author(s): Nagaya H, Gotoh A, Kanno T, Nishizaki T
  • Journal Title: J Urol Volume: 189 Issue: 1 Pages: 321-328
  • DOI: 10.1016/j.juro.2012.08.193
  • Peer Reviewed
• [Journal Article] Fiber-substituted conditionally replicating adenovirus for oncolysis of human renal carcinoma cells. (2012)
  • Author(s): Nagaya H, Tagawa M, Hiwasa K, Terao S, Kanno T, Nishizaki T Gotoh A.
  • Journal Title: Anticancer Res Volume: 32 Pages: 2985-9
  • Peer Reviewed
• [Presentation] 生薬成分と分子標的薬を用いた併用療法によるがん治療のための基礎研究 (2014)
  • Author(s): 長屋寿雄,中川佑介,宮田剛彰,下村欣也,小山倫浩,後藤章暢
  • Organizer: 第23回泌尿器科分子・細胞研究会
  • Place of Presentation: 山形
• [Presentation] Crosstalk between ERK and Akt pathways in malignant mesothelioma cells(中皮腫細胞株におけるERKシグナルとAktシグナルのクロストークの検討) (2013)
  • Author(s): 長屋寿雄,中川佑介,宮田剛彰,下村欣也,日和佐一弘,中野孝司,西崎知之,後藤章暢
  • Organizer: 第72回日本癌学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Antitumor Effect of Alpha1-Adrenoceptor Blockers on Human Urological Cancer Cells(泌尿器系癌に対するα1ブロッカーの抗腫瘍効果の検討) (2013)
  • Author(s): 中川佑介,長屋寿雄,宮田剛彰,下村欣也,日和佐一弘,西崎知之,後藤章暢
  • Organizer: 第72回日本癌学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Oncolytic effects of a fiber knob substituted adenovirus vector to human lung cancer cells (2013)
  • Author(s): Nagaya H, Kanno T, Hiwasa K, Nakagawa Y, Shimomura K, Nakano T, Nishizaki T, Tagawa M, Gotoh A
  • Organizer: 第19回日本遺伝子治療学会年次学術集会
  • Place of Presentation: 岡山
• [Presentation] Oncolytic effects of a fiber knob substituted adenovirus vector to human lung cancer cells. (2013)
  • Author(s): Nagaya H, Kanno T, Hiwasa K, Nakagawa Y, Shimomura K, Nakano T, Nishizaki T, Tagawa M, Gotoh A
  • Organizer: 第19回日本遺伝子治療学会年次学術集会
  • Place of Presentation: 岡山
• [Presentation] Crosstalk between ERK and Akt pathways in malignant mesothelioma cells(中皮腫細胞株におけるERKシグナルとAktシグナルのクロストークの検討). (2013)
  • Author(s): 長屋寿雄, 中川佑介, 宮田剛彰, 下村欣也, 日和佐一弘, 中野孝司, 西崎知之, 後藤章暢
  • Organizer: 第72回日本癌学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Antitumor Effect of Alpha1-Adrenoceptor Blockers on Human Urological Cancer Cells(泌尿器系癌に対するα１ブロッカーの抗腫瘍効果の検討). (2013)
  • Author(s): 中川佑介, 長屋寿雄, 宮田剛彰, 下村欣也, 日和佐一弘, 西崎知之, 後藤章暢
  • Organizer: 第72回日本癌学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Oncolytic effects of a fiber knob substituted adenovirus vector to human bladder carcinoma cells. (2012)
  • Author(s): Nagaya H, Terao S, Hiwasa K, Tagawa M, Gotoh A.
  • Organizer: 第18回日本遺伝子治療学会年次学術集会
  • Place of Presentation: ホテル熊本テルサ（熊本）
• [Presentation] 腎癌細胞株に対するファイバー改変アデノウイルスベクターによるin vitroでの抗腫瘍効果の検討 (2012)
  • Author(s): 長屋寿雄、寺尾秀治、日和佐一弘、田川雅敏、後藤章暢
  • Organizer: 第21回 泌尿器科分子・細胞研究会
  • Place of Presentation: 札幌