Investigation on mucosa-derived substance that enhances spontaneous contractions of the urinary bladder in the rat with bladder outlet obstruction

• Project/Area Number: 23592359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: University of Fukui
• Principal Investigator: AKINO HIRONOBU 福井大学, 医学部, 准教授 (90159335)
• Co-Investigator(Kenkyū-buntansha): YOKOYAMA Osamu 福井大学, 医学部, 教授 (90242552) ; WATANABE Nozomu 福井大学, 医学部附属病院, 医員 (80572429)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 下部尿路閉塞 / 膀胱粘膜 / 膀胱自律収縮 / 生理学 / ストレス / 自律収縮 / アデノシン3リン酸（ATP） / プロスタグランディンE2（PGE2）

Research Abstract

We examined what the mucosa-derived substance that can act to enhance spontaneous contractions (SCs) of the urinary bladder is in the rat with bladder outlet obstruction (BOO).
The SCs in bladder strips from BOO rats were associated with the amount of prostaglandin (PG) E2 released from the mucosa in vitro: The amount of PGE2 released from the mucosa was increased in BOO rats in vivo: The non-voiding contraction (NVC) that was regarded as a surrogate of SCs in the bladder wall in vivo was associated with mucosa-derived PGE2. The mucosa-derived substance to enhance SCs may be PGE2. We also found out that the presence of the intact nervous system innervating lower urinary tract and the up-regulation of the expression of cyclooxygenase-2 in the bladder wall were associated with the increased amount of PGE2 released from the mucosa induced by BOO.

Research Products

• [Journal Article] Antidiuretic effect of antimuscarinic agents in rat model depends on C-fibre afferent nerves in the bladder. (2013)
  • Author(s): Watanabe N, Akino H, Kurokawa T, Taga M, Yokokawa R, Tanase K, Nagase K, Yokoyama O.
  • Journal Title: BJU Int Volume: 112 Issue: 1 Pages: 131-136
  • DOI: 10.1111/j.1464-410x.2012.11747.x
  • Peer Reviewed
• [Journal Article] IPSS is lower in hypertensive patients treated with angiotensin-II receptor blocker: posthoc analyses of a lower urinary tract symptoms population. (2013)
  • Author(s): Ito H, Taga M, Tsuchiyama K, Akino H, Yokoyama O.
  • Journal Title: Neurourol Urodyn Volume: 32 Issue: 1 Pages: 70-74
  • DOI: 10.1002/nau.22267
  • Peer Reviewed
• [Journal Article] 膀胱知覚における筋層自律収縮の役割-上皮と間質細胞の関与も含めて- (2012)
  • Author(s): 秋野裕信,長瀬桂子,田中一平,渡邉望,大山伸幸,三輪吉司,横山修
  • Journal Title: 排尿障害プラクティス Volume: 20 Pages: 32-38
  • Peer Reviewed
• [Journal Article] 膀胱機能をみる尿流動態検査 (2012)
  • Author(s): 秋野裕信,横山修
  • Journal Title: 泌尿器外科 Volume: 25 Pages: 2265-2271
  • Peer Reviewed
• [Journal Article] 膀胱知覚における筋層自律収縮の役割－上皮と間質細胞の関与も含めて－ (2012)
  • Author(s): 秋野裕信、長瀬桂子、田中一平、渡邉望、大山伸幸、三輪吉司、横山 修
  • Journal Title: 排尿障害プラクティス Volume: 20 Pages: 32-38
  • Peer Reviewed
• [Journal Article] Antimuscarinics suppress adenosine triphosphate and prostaglandin e2 release from urothelium with potential improvement in detrusor overactivity in rats with cerebral infarction (2011)
  • Author(s): Yokoyama O, Tanaka I, Kusukawa N, Yamauchi H, Ito H, Aoki Y, Oyama N, Miwa Y, Akino H
  • Journal Title: J Urol Volume: 185 Pages: 2392-2397
  • Peer Reviewed
• [Journal Article] Male LUTSと超音波推定膀胱重量 (2011)
  • Author(s): 秋野裕信,横山修
  • Journal Title: Urology Today Volume: 18 Pages: 46-50
• [Journal Article] Antimuscarinics suppress adenosine triphosphate and prostaglandin E2 release from urothelium with potential improvement in detrusor overactivity in rats with cerebral infarction (2011)
  • Author(s): Yokoyama O, Tanaka I, Kusukawa N, Yamauchi H, Ito H, Aoki Y, Oyama N, Miwa Y, Akino H
  • Journal Title: J Urol Volume: 185 Issue: 6 Pages: 2392-2397
  • DOI: 10.1016/j.juro.2011.02.048
  • Peer Reviewed
• [Journal Article] Male LUTS と超音波推定膀胱重量 (2011)
  • Author(s): 秋野 裕信, 横山 修
  • Journal Title: Urology Today Volume: 18 Pages: 46-50
• [Presentation] 下部尿路閉塞ラットのnon-voiding contraction(NVC)と膀胱内ATP・PGE2放出量に対するC線維脱感作・α1遮断薬の効果 (2014)
  • Author(s): 秋野裕信,長瀬桂子,横川竜生,渡邉望,石田泰一,棚瀬和弥,大山伸幸,三輪吉司,横山修
  • Organizer: 第101回日本泌尿器科学会
  • Place of Presentation: 神戸
  • Year and Date: 2014-04-26
• [Presentation] 下部尿路閉塞ラットのnon-voiding contraction(NVC)と膀胱内ATP・PGE2放出量に対するC線維脱感作・α1遮断薬の効果 (2014)
  • Author(s): 秋野 裕信, 長瀬 桂子, 横川 竜生, 渡邉 望, 石田 泰一, 棚瀬 和弥, 大山 伸幸, 三輪 吉司, 横山 修
  • Organizer: 第101回日本泌尿器科学会総会
  • Place of Presentation: 神戸市（神戸国際会議場、神戸国際展示場、神戸ポートピアホテル）
• [Presentation] In vitroラット全膀胱における膀胱内ATP・PGE2放出量と自発収縮:下部尿路閉塞の影響 (2013)
  • Author(s): 長瀬桂子,秋野裕信,横川竜生,青木芳隆,三輪吉司,横山修
  • Organizer: 第20回日本排尿機能学会
  • Place of Presentation: 静岡
  • Year and Date: 2013-09-20
• [Presentation] In vitro全膀胱の膀胱腔外PGE2放出と自発収縮に対する下部尿路閉塞・α1遮断薬の影響 (2013)
  • Author(s): 秋野裕信,長瀬桂子,横川竜生,横井聡始,関雅也,伊藤秀明,大山伸幸,横山修
  • Organizer: 第20回日本排尿機能学会
  • Place of Presentation: 静岡
  • Year and Date: 2013-09-20
• [Presentation] Non-voiding contractions and intravesical release of adenosine triphosphate and prostaglandin e2 in a rat model of bladder outlet obstruction : effects of resiniferatoxin and anα1-blocker, naftopidil (2013)
  • Author(s): Akino H, Nagase K, Yokokawa R, Ito H, Aoki Y, Oyama N, Yokoyama O
  • Organizer: 43th Annual Meeting of the international Continence Society
  • Place of Presentation: Barcelona(SPA)
  • Year and Date: 2013-08-28
• [Presentation] The influence of the mucosa and bladder outlet obstruction on the effect of cyclooxygenase inhibitor, indomethacin, on spontaneous contractile activity in the rat bladder (2013)
  • Author(s): Nagase K, Akino H, Yokokawa R, Ishida H, Yokoyama O
  • Organizer: 43th Annual Meeting of the international Continence Society
  • Place of Presentation: Barcelona(SPA)
  • Year and Date: 2013-08-28
• [Presentation] Non-voiding contractions and intravesical release of adenosine triphosphate and prostaglandin e2 in a rat model of bladder outlet obstruction: effects of resiniferatoxin and an α1-blocker, naftopidil (2013)
  • Author(s): H.Akino, K.Nagase, R.Yokokawa, H.Ito, Y.Aoki, N.Oyama, Y.Miwa, O.Yokoyama
  • Organizer: The 43rd Annual Meeting of the International Continence Society
  • Place of Presentation: バルセロナ（スペイン）
• [Presentation] The influence of the mucosa and bladder outlet obstruction on the effect of cyclooxygenase inhibitor, indomethacin, on spontaneous contractile activity in the rat bladder (2013)
  • Author(s): K.Nagase, H.Akino, R.Yokokawa, H.Ishida, O.Yokoyama
  • Organizer: The 43rd Annual Meeting of the International Continence Society
  • Place of Presentation: バルセロナ（スペイン）
• [Presentation] In vitroラット全膀胱における膀胱内ATP・PGE2放出量と自発収縮：下部尿路閉塞の影響 (2013)
  • Author(s): 長瀬 桂子, 秋野 裕信, 横川 竜生, 青木 芳隆, 三輪 吉司, 横山 修
  • Organizer: 第20回日本排尿機能学会
  • Place of Presentation: 静岡市（グランシップ）
• [Presentation] In vitro 全膀胱の膀胱腔外PGE2放出と自発収縮に対する下部尿路閉塞・α1遮断薬の影響 (2013)
  • Author(s): 秋野 裕信, 長瀬 桂子, 横川 竜生, 横井 聡始, 関 雅也, 伊藤 秀明, 大山 伸幸, 横山 修
  • Organizer: 第20回日本排尿機能学会
  • Place of Presentation: 静岡市（グランシップ）
• [Presentation] Effects of C-fibre's desensitization and alpha 1-blocker on non-voiding contractions in a rat model of bladder outlet obstruction (2012)
  • Author(s): Akino H, Nagase K, Watanabe N, Tanase K, Oyama N, Miwa Y, Yokoyama O
  • Organizer: 42th Annual Meeting of the international Continence Society
  • Place of Presentation: Beijing(CHN)
  • Year and Date: 2012-10-17
• [Presentation] Non-voiding Contractions Induced by Bladder Outlet Obstruction : Effects of C-fiber's Desensitization and an Alpha 1-adrenoceptor Blocker Naftopidil (2012)
  • Author(s): Akino H,Nagase K, Watanabe N, Tanase K, Oyama N, Miwa Y, Yokoyama O
  • Organizer: 32nd congress of the Societe Internationale d'Urologie
  • Place of Presentation: Fukuoka(JPN)
  • Year and Date: 2012-10-01
• [Presentation] レジニフェラトキシン全身投与が下部尿路閉塞ラットのnon-voiding contractionと膀胱腔内ATP・プロスタグランディンE2放出量に及ぼす効果について (2012)
  • Author(s): 秋野裕信,長瀬桂子,横川竜生,渡邉望,伊藤秀明,青木芳隆,大山伸幸,三輪吉司,横山修
  • Organizer: 第19回日本排尿機能学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-08-31
• [Presentation] α1遮断薬の症状改善メカニズムを探る (2012)
  • Author(s): 秋野裕信
  • Organizer: 第19回日本排尿機能学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-08-30
• [Presentation] 膀胱自律収縮に関する研究から過活動膀胱治療の新規標的を探求する (2012)
  • Author(s): 秋野裕信,長瀬桂子,田中一平,横山修
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2012-04-22
• [Presentation] Effects of C-fibre’s desensitization and alpha 1-blocker on non-voiding contractions in a rat model of bladder outlet obstruction (2012)
  • Author(s): Akino H, Nagase K, Watanabe N, Tanase K, Oyama N, Miwa Y, Yokoyama O.
  • Organizer: ICS 2012
  • Place of Presentation: Beijing, China
• [Presentation] Non-voiding Contractions Induced by Bladder Outlet Obstruction: Effects of C-fiber’s Desensitization and an Alpha 1-adrenoceptor Blocker Naftopidil. (2012)
  • Author(s): Akino H, Nagase K, Watanabe N, Tanase K, Oyama N, Miwa Y, Yokoyama O
  • Organizer: 32nd congress of the Societe Internationale d’Urologie(SIU)
  • Place of Presentation: Fukuoka, JPN
• [Presentation] レジニフェラトキシン全身投与が下部尿路閉塞ラットのnon-voiding contractionと膀胱腔内ATP・プロスタグランディンE2放出量に及ぼす効果について (2012)
  • Author(s): 秋野裕信、長瀬桂子、横川竜生、渡邉望、伊藤秀明、青木芳隆、大山伸幸、三輪吉司、横山 修
  • Organizer: 第19回日本排尿機能学会
  • Place of Presentation: 名古屋市
• [Presentation] 膀胱自律収縮に関する研究から過活動膀胱治療の新規標的を探求する (2012)
  • Author(s): 秋野裕信、長瀬桂子、田中一平、横山 修
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: 横浜市
  • Invited
• [Presentation] α1遮断薬の症状改善メカニズムを探る (2012)
  • Author(s): 秋野裕信
  • Organizer: 第19回日本排尿機能学会
  • Place of Presentation: 名古屋市
  • Invited
• [Presentation] 脊髄損傷ラットにおけるα1-Blockerの静脈投与における膀胱活動の変化について (2011)
  • Author(s): 秋野裕信
  • Organizer: 第18回日本排尿機能学会
  • Place of Presentation: 福井
  • Year and Date: 2011-09-16
• [Presentation] 脳梗塞急性期における膀胱壁のATP放出メカニズムの亢進とC-fiberの関与について (2011)
  • Author(s): 秋野裕信
  • Organizer: 第18回日本排尿機能学会
  • Place of Presentation: 福井
  • Year and Date: 2011-09-16
• [Presentation] ATP and PGE2 release from the rat bladder in-vitro and in-vivo : the effect of bladder outlet obstruction and the association with bladder weight,spontaneous contraction or non-voiding contractions (2011)
  • Author(s): Akino H, Nagase K, Watanabe N, Yamauchi H, Oyama N, Miwa Y, Yokoyama O
  • Organizer: 41th Annual Meeting of the international Continence Society
  • Place of Presentation: Glasgow(GBR)
  • Year and Date: 2011-08-29
• [Presentation] Effects of A1-blocker on the release of ATP and prostaglandin E2 from the urethral epithelium in boo rats (2011)
  • Author(s): Nagase K, Tanaka I, X Zha, Kusukawa N, Ito H, Aoki Y, Oyama N, Miwa Y, Akino H, Yokoyama O
  • Organizer: 41th Annual Meeting of the international Continence Society
  • Place of Presentation: Glasgow(GBR)
  • Year and Date: 2011-08-29
• [Presentation] 下部尿路閉塞でのin-vitro膀胱自律収縮と膀胱粘膜由来ATP放出量 (2011)
  • Author(s): 秋野裕信,長瀬桂子,田中一平,渡邉望,石田泰一,棚瀬和弥,大山伸幸,三輪吉司,横山修
  • Organizer: 第99回日本泌尿器科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-04-21
• [Presentation] 脊髄損傷ラットにおけるα1-blockerの静脈投与における膀胱活動の変化について (2011)
  • Author(s): 秋野 裕信
  • Organizer: 第18回日本排尿機能学会
  • Place of Presentation: 福井
• [Presentation] 脳梗塞急性期における膀胱壁のＡＴＰ放出メカニズムの亢進とC-fiberの関与について (2011)
  • Author(s): 秋野 裕信
  • Organizer: 第18回日本排尿機能学会
  • Place of Presentation: 福井
• [Presentation] ATP and PGE2 release from the rat bladder in-vitro and in-vivo:the effect of bladder outlet obstruction and the association with bladder weight,spontaneous contraction or non-voiding contractions (2011)
  • Author(s): H.Akino
  • Organizer: 41th Annual Meeting of the international Continence Society(ICS)
  • Place of Presentation: Glasgow(GBR)
• [Presentation] Effects of A1-blocker on the release of ATP and prostaglandin E2 from the urethral epithelium in boo rats (2011)
  • Author(s): H.Akino
  • Organizer: 41th Annual Meeting of the international Continence Society(ICS)
  • Place of Presentation: Glasgow(GBR)
• [Presentation] 下部尿路閉塞でのin-vitro膀胱自律収縮と膀胱粘膜由来ATP放出量 (2011)
  • Author(s): 秋野 裕信, 長瀬 桂子, 田中 一平, 渡邉 望, 石田 泰一, 棚瀬 和弥, 大山 伸幸, 三輪 吉司, 横山 修
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋
• [Remarks]
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• [Remarks] 福井大学医学部研究活動一覧
  • URL: http://www.med.u-fukui.ac.jp/home/ufms/file/kenkyu/welcome.html
• [Remarks] 福井大学医学部研究活動一覧
  • URL: http://www.med.u-fukui.ac.jp/home/ufms/file/kenkyu/welcome.html