Tissue Engineering for lower urinary tract by autologous bone marrow stem cell and adispose tissue

• Project/Area Number: 23592363
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Shinshu University
• Principal Investigator: ISHIZUKA Osamu 信州大学, 医学部, 准教授 (20184541)
• Co-Investigator(Kenkyū-buntansha): IMAMURA Tetsuya 信州大学, 医学部, 講師 (00467143)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 移植・再生医療 / 骨髄幹細胞 / 脂肪細胞 / 膀胱 / 尿道括約筋 / 脂肪幹細胞 / 再生医学 / 細胞・組織 / 生体機能利用 / 脳神経疾患

Research Abstract

From the clinical point of view, urethral sphincter reconstruction is important to prevent urinary incontinence in the field of bladder reconstruction. At first, we could culture and proliferate the bone-marrow-derived mesenchymal stem cells derived from the femur of rabbit and abdominal adipose tissue derived stem cells. The cultured stem cells were implanted into the injured urethral sphincter, and we could found the reconstruction of the urethral sphincter by histological methods (immunohistochemistry, RT-PCR method) and functional study.

Research Products

• [Journal Article] Implantation of autologous adipose-derived cells reconstructs functional urethral sphincters in rabbit cryo-injured urethra (2014)
  • Author(s): Sudha, G.S., Imamura, T., Ishizuka, O., Zhang, L., Yamagishi, T., Yokoyama, H., Minagawa, T., Ogawa, T., Kurizaki, Y., Kato, H., Nishizawa, O.
  • Journal Title: Tissue Eng. Part A Volume: (Epub ahead of print)
  • NAID: 120007100147
  • Peer Reviewed
• [Journal Article] Editorial commnet from Dr. Ishizuka to periurethral injection of autologous adipose-derived regenerative cells for the treatment of male stress incontinence: report of three initial cases. (2013)
  • Author(s): Ishizuka O
  • Journal Title: Int.J.Urol. Volume: 20 Issue: 2 Pages: 240-240
  • DOI: 10.1111/j.1442-2042.2012.03145.x
  • Peer Reviewed
• [Journal Article] Bone marrow-derived cells implanted into radiation-injured urinary bladders reconstructe functional bladder tissues in rats (2012)
  • Author(s): Imamura, T., Ishizuka, O., Zhang, L., Hida, S., Sudha, G.S., Kato, H., Nishizawa, O.
  • Journal Title: Tissue Eng Part A Volume: 18(15-16) Pages: 1698-709
  • NAID: 120007110571
  • Peer Reviewed
• [Journal Article] Bone Marrow-Derived Cells Implanted into Radiation-Injured Urinary Bladders Reconstruct Functional Bladder Tissues in Rats (2012)
  • Author(s): Imamura T, Ishizuka O, Lei Z, Hida S, Sudha GS, Kato H, Nishizawa O
  • Journal Title: Tissue Eng Part A Volume: (in press) Issue: 15-16 Pages: 1698-1709
  • DOI: 10.1089/ten.tea.2012.0061
  • NAID: 120007110571
  • Peer Reviewed
• [Journal Article] Autologous bone marrow-derived cells regenerate urethral sphincters (2012)
  • Author(s): Imamura, T., Ishizuka, O., Nishizawa, O.
  • Journal Title: LUTS Volume: 4 Issue: s1 Pages: 87-94
  • DOI: 10.1111/j.1757-5672.2011.00136.x
  • Peer Reviewed
• [Journal Article] Editorial Comment from Dr Ishizuka to periurethral injection of autologous adipose-derived regenerative cells for the treatment of male stress urinary incontinence: report of three initial cases (2012)
  • Author(s): Ishizuka O
  • Journal Title: Int. J.Urol. Volume: Mar 27 Issue: 7 Pages: 659-659
  • DOI: 10.1111/j.1442-2042.2012.03011.x
  • NAID: 10030824000
  • Peer Reviewed
• [Journal Article] Implantation of autologous bone marrow-derived cells reconstructs functional urethral sphincters in rabbits (2011)
  • Author(s): Imamura, T., Ishizuka, O., Kinebuchi, Y., Kurizaki, Y., Nakayama, T., Ishikawa, M., Nishizawa, O.
  • Journal Title: Tissue Eng. Part A Volume: 17(7-8) Pages: 1069-81
  • NAID: 120007110472
  • Peer Reviewed
• [Journal Article] Implantation of autologous bone-marrow-derived cells reconstructs functional urethral sphincters in rabbits. (2011)
  • Author(s): Imamura T, Ishizuka O, Kinebuchi Y, Nakayama T, Ishikawa M, Nishizawa O
  • Journal Title: Tissue Eng PartA Volume: 17 Pages: 1069-81
  • NAID: 120007110472
  • Peer Reviewed
• [Presentation] The implantation of autologous adipose-derived cells makes the functional regeneration of frozen-injured rabbit urethra (2013)
  • Author(s): Sudha, G., Imamura, T., Ishizuka, O., Zhang, L., Noguchi, W., Yamagishi, T., Yokoyama, H., Kurizaki, Y., Nishizawa, O., Andersson, K.E.
  • Organizer: 33th congress of the Societe Internationale d'Urologie
  • Place of Presentation: Vancouver(Urology 82(9) suppl , S96, 2013). (presented by O.Ishizuka)
• [Presentation] The implantation of autologous adipose-derived celss makes the functional regeneration of frozen-injured rabbit urethra (2013)
  • Author(s): Sudha, G., Imamura, T., Ishizuka, O., Zhang, L., Noguchi, W., Yamagishi, T., Yokoyama, H., Kurizaki, Y., Nishizawa, O., Andersson, K.E.
  • Organizer: 33th congress of the Societe Internationale d’Urologie
  • Place of Presentation: Vancouver, Canada
• [Presentation] Implantation of Bone Marrow-derived cells recovers tissue structures and functions of radiation-injured urinary bladders in rats (2012)
  • Author(s): Imamura, T., Ishizuka, O., Kurizaki, Y., Yokoyama, H., Noguchi, W., Yamagishi, T., Zhang, L., Kato, H., Nishizawa, O.
  • Organizer: 2012 annual meeting of American Urological Association
  • Place of Presentation: Atlanta
• [Presentation] Implantation of Bone Marrow-derived cells recovers tissue structures and functions of radiation-injured urinary bladders in rats. (2012)
  • Author(s): Imamura, T., Ishizuka, O., Kurizaki, Y., Yokoyama, H., Noguchi, W., Yamagishi, T., Zhang, L., Kato, H., Nishizawa, O.
  • Organizer: 2012 annual meeting of American Urological Asscociation
  • Place of Presentation: Atlanta, USA
• [Presentation] Implantation of autologous bone marrow-derived cells recovers urethral sphincter in rabbits (2011)
  • Author(s): Imamura, T., Ishizuka, O., Kurizaki, Y., Ishikawa, M., Noguchi, A., Zhang, L., Ichino, M., Kato, H., Nishizawa, O.
  • Organizer: The 41^<th> Annual Meeting of the International Continence Society
  • Place of Presentation: Glasgow
• [Presentation] Implantation of autologous bone marrow -derived cells recovers urethral spinster composed of striated and smooth muscle layers in rabbits (2011)
  • Author(s): Imamura T, Ishizuka O et al.
  • Organizer: 41st annual meeting of the international continence society
  • Place of Presentation: Glasgow, UK
• [Book] 下部尿路機能ポケットマニュアル (2012)
  • Author(s): 石塚 修、井川靖彦
  • Publisher: メデイカルレビュー