Evaluation of bladder and sexual function in rat water avoidance stress model.

• Project/Area Number: 23592367
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: TAKAO Tetsuya 大阪大学, 医学(系)研究科(研究院), 講師 (30379177)
• Co-Investigator(Kenkyū-buntansha): TSUJIMURA Akira 大阪大学, 大学院医学系研究科, 准教授 (40294053) ; MIYAGAWA Yasushi 大阪大学, 大学院医学系研究科, 講師 (70362704) ; NONOMURA Norio 大阪大学, 大学院医学系研究科, 教授 (30263263)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ストレス / 頻尿 / ラット / 過活動膀胱 / 性機能障害

Research Abstract

Water avoidance stress (WAS) is a potent psychological stressor and it is associated with visceral hyperalgesia. We investigated the voiding pattern and cyclooxygenase-2 (Cox-2) expression in a rat bladder model of WAS. Metabolic cage analysis and cystometrography showed significantly shorter intervals and less volume of voiding in WAS rats. Significantly higher expression of Cox-2 mRNA was verified by reverse transcription PCR. Immunohistochemistry and western blotting showed significantly higher Cox-2 protein levels in WAS bladders. Furthermore, immunohistochemistry showed high Cox-2 expression exclusively in smooth muscle cells. All WAS-induced changes were reduced by Cox-2 inhibitor pretreatment. Chronic stress might cause frequency through Cox-2 gene upregulation in bladder smooth muscle cells. Further study of Cox-2 in the WAS bladder might provide novel therapeutic modalities for interstitial cystitis.

Research Products

• [Journal Article] Water avoidance stress induces frequency through cyclooxygenase-2 expression : a bladder rat model (2012)
  • Author(s): Yamamoto K, Takao T, Nakayama J, Kiuchi H, Okuda H, Fukuhara S, Yoshioka I, Matsuoka Y, Miyagawa Y, Tsujimura A, Nonomura N
  • Journal Title: Int J Urol Volume: 19(2) Issue: 2 Pages: 155-62
  • DOI: 10.1111/j.1442-2042.2011.02905.x
  • NAID: 10031184798
  • Peer Reviewed
• [Presentation] Water Avoidance StressラットにおけるFesoterodineの効果 (2012)
  • Author(s): 山本圭介、奥田英伸、福原慎一郎、吉岡厳、木内寛、高尾徹也、宮川康、辻村晃、中山治郎、松岡庸洋、野々村祝夫
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-04-21
• [Presentation] COX-2 inhibitorはWater Avoidance Stressラットの頻尿を改善する (2011)
  • Author(s): 山本圭介、奥田英伸、福原慎一郎、吉岡巌、松岡庸洋、高尾徹也,宮川康,辻村晃,木内寛、中山治郎、野々村祝夫
  • Organizer: 第18回日本排尿機能学会
  • Place of Presentation: 福井市
  • Year and Date: 2011-09-16
• [Presentation] Water Avoidance Stressラットモデルの頻尿とCOX-2の関連 (2011)
  • Author(s): 山本圭介,奥田英伸,福原慎一郎,中山治郎,高尾徹也,宮川康,辻村晃,奥山明彦,木内寛
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-04-21
• [Presentation] Water Avoidance Stressモデルラットの頻尿とCOX-2の関連
  • Author(s): 山本圭介, 奥田英伸, 福原慎一郎, 松岡庸洋, 高尾徹也, 宮川康, 辻村晃, 木内寛, 中山治郎, 野々村祝夫
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋市