The elucidation of functional roles of GABA receptor during spermatogenesis process

Research Project

Project/Area Number	23592387
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Urology
Research Institution	Osaka Medical College
Principal Investigator	KANBARAN Kiyoto 大阪医科大学, 医学部, 非常勤講師 (40298758)
Co-Investigator(Kenkyū-buntansha)	MORI Yoshiaki 関西福祉科学大学, 保健医療学部, 教授 (70268192)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	GABAA受容体 / 精子形成過程 / 核内移行 / GABA A受容体 / GABA受容体 / 生殖細胞形成過程 / 核内受容体
Research Abstract	We investigated whether the alpha 1 subunit of GABAA receptor which was cell membranous receptor would be translocated into the nucleus during spermatogenesis process using Western blotting and immunoelectron microscopy. These findings suggested that the alpha 1 subunit which was translocated into the nucleus might function as a gene transcription factor in the area of condensed chromatin during the maturation of germ cells. Furthermore, it was thought that the nuclear translocation of the alpha 1 subunit received some kind of genetic control in the transfection experiment of the alpha 1 subunit.