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Epigenetics of Cdx2 gene on the extraembryonic cell lineage and human trophoblastic stem cell.

Research Project

Project/Area Number 23592390
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

SUGAWARA Junichi  東北大学, 東北メディカル・メガバンク機構, 教授 (60280880)

Co-Investigator(Kenkyū-buntansha) OKAE Hiroaki  東北大学, 医学系研究科, 助教 (10582695)
HIURA Hitoshi  東北大学, 医学系研究科, 助教 (70451523)
ARIMA Takahiro  東北大学, 医学系研究科, 教授 (80253532)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords胎盤幹細胞 / エピジェネティクス / 細胞系譜
Research Abstract

The increased expression of Cdx2, and more differentiated embryonic body tissue to the blastocyst stage, extra-embryonic tissue to differentiate into placental tissue (TE) determines the cell fate. In this study, using 1) placental stem (TS) cells, and subjected to analysis for histone modification and DNA methylation of Cdx2 gene promoter region. As a result, the DNA methylation of the promoter region of the gene Cdx2 be low methylation status, that histone modifications are also active was found. As a result of using a TS cell line knockdown 2) of the Cdx2 gene was observed, were analyzed for cell cycle and morphological changes, G1 arrest was observed and reduced growth potential. From this fact, the differentiation and maintenance of TS cells, that Cdx2 gene plays an important role has been suggested.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (8 results)

All 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] 生殖補助医療とエピジェネティクス2014

    • Author(s)
      樋浦仁、有馬隆博
    • Journal Title

      エピジェネティクス―基礎研究から産業応用への展望―(シーエムシー出版)

      Volume: (印刷中)

    • Related Report
      2013 Final Research Report
  • [Journal Article] 生殖医療と児の奇形、エピジェネティクス異常2014

    • Author(s)
      千葉初音、有馬隆博
    • Journal Title

      医学のあゆみ(医歯薬出版)

      Volume: (印刷中)

    • Related Report
      2013 Final Research Report
  • [Journal Article] 生殖補助医療とエピジェネティクス2014

    • Author(s)
      樋浦仁、有馬隆博
    • Journal Title

      エピジェネティクス―基礎研究から産業応用への展望―(シーエムシー出版)

      Volume: -

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 生殖医療と児の奇形、エピジェネティクス異常2014

    • Author(s)
      千葉初音、有馬隆博
    • Journal Title

      医学のあゆみ(医歯薬出版)

      Volume: -

    • Related Report
      2013 Annual Research Report
  • [Journal Article] DNA methylation errors in imprinting disorders and assisted reproductive technologies.2013

    • Author(s)
      Chiba H, Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Arima T.
    • Journal Title

      Pediatrics international.

      Volume: 55 Issue: 5 Pages: 542-549

    • DOI

      10.1111/ped.12185

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of recombinant human soluble thrombomodulin in severe postpartum hemorrhage with disseminated intravascular coagulation.2013

    • Author(s)
      Sugawara J, Suenaga K, Hoshiai T, Sato T, Nishigohri H, Nagase S and Yaegashi N.
    • Journal Title

      Clin Appl Thromb/Hemost

      Volume: 19(5) Issue: 5 Pages: 557-561

    • DOI

      10.1177/1076029612443305

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ヒト生殖補助医療(ART)とエピジェネティクスの異常2013

    • Author(s)
      千葉初音、岡江寛明、有馬隆博
    • Journal Title

      遺伝子医学MOOK(メディカルドゥ)

      Volume: 25 Pages: 178-183

    • Related Report
      2013 Final Research Report
  • [Journal Article] ヒト生殖補助医療(ART)とエピジェネティクスの異常2013

    • Author(s)
      千葉初音、岡江寛明、有馬隆博
    • Journal Title

      遺伝子医学MOOK(メディカルドゥ)

      Volume: 25 Pages: 178-183

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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