The systemic and basic study on the clinical application of the nuclear transfer method.

Research Project

Project/Area Number	23592398
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	University of Yamanashi
Principal Investigator	HIRATA Shuji 山梨大学, 総合研究部, 教授 (00228785)
Co-Investigator(Kenkyū-buntansha)	FUKASAWA Hiroko 山梨大学, 総合研究部, 助教 (60362068) SHODA Tomoko 山梨大学, 総合研究部, 助教 (50345716)
Project Period (FY)	2011-04-28 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	体細胞核移植 / ドナー体細胞 / 卵細胞質 / ミトコンドリア / 初期発生 / IVM / 凍結保存 / ミトコンドリア動態 / 体細胞核移植胚 / 培養条件 / ntES 細胞 / 成熟培養 (IVM) / 凍結除核卵 / 未熟卵 / 成熟培養 / 再生医療
Outline of Final Research Achievements	The final destination of the present study is clinical application of the nuclear transferred embryonal stem (ntES) cells. The systemic and basic study on the "nt" was carried out to establish the methodology; "1) in vitro maturation (IVM) of the immature oocyte, 2) cryopreservation of the enucleated IVM oocyte, and 3) reconstruction of the cloned embryo using the enucleated IVM oocyte and donor nucleus. However, it was indicated that the early development rate of the cloned embryo was very low, and the rate should be improved for further investigation. Therefore, we study the factors related to the development thoroughly. As a result, the dynamics of the cytoplasm of the cloned embryo was important for the development. Further study revealed that the changes in the distribution of the mitochondria were a key factor. From the present study, the strategy that improve the early development of the cloned embryo was established.