Relaxin and immune abnormality in human reproductive failure
| Project/Area Number |
23592403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kobe University |
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Principal Investigator |
YAMADA Hideto 神戸大学, 医学(系)研究科(研究院), 教授 (40220397)
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| Co-Investigator(Kenkyū-buntansha) |
NAKABAYASHI Koji 神戸大学, 医学部附属病院, 講師 (80362789)
MAKIHARA Natsuko 神戸大学, 医学部附属病院, 助教 (30593991)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リラキシン / 子宮筋腫 / 流産 / アポトーシス / 絨毛外栄養膜細胞 / 生殖 / 免疫 |
|---|
| Research Abstract |
The study was conducted to evaluate the effects of human relaxin on apoptosis in the human trophoblast derived HTR-8/SV neo cell line, which is a possible model of human extravillous trophoblasts (EVTs). HTR-8/SV neo cells were treated with different doses of human recombinant (rH2) relaxin. RXFP1 and RXFP2 mRNA expression was observed in HTR-8/SV neo cells. Compared with untreated control cultures, treatment with rH2 relaxin, decreased TUNEL-positive rate in HTR-8/SV neo cells was observed. Western blot analysis revealed that treatment with rH2 relaxin decreased the expression of caspase-3 and cleaved PARP, but in contrast increased Bcl-2 expression in those cells. These results suggest that rH2 relaxin has anti-apoptotic effects on HTR8/SV neo cells by decreasing pro-apoptotic caspase-3 and cleaved PARP expression and up-regulating anti-apoptotic Bcl-2 expression.
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Report
(4 results)
Research Products
(136 results)
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[Journal Article] Novel MRI finding for diagnosis of invasive placenta praevia: evaluation of findings for 65 patients using clinical and histopathological correlations2014
Author(s)
Ueno Y, Kitajima K, Kawakami F, Maeda T, Suenaga Y, Takahashi S, Matsuoka S, Tanimura K, Yamada H,Ohno Y, Sugimura K
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Journal Title
Eur Radiol.
Volume: 24
Issue: 4
Pages: 881-888
DOI
Related Report
Peer Reviewed
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[Journal Article] Dried umbilical cord is a novel diagnostic material for retrospective diagnosis of intrauterine enterovirus infection2014
Author(s)
Morioka I, Matsumoto M, Miwa A, Yokota T, Matsuo K, Koda T, Nagasaka M, Shibata A, Fujita K,Yamane M, Yamada H, Enomoto M, Chikahira M, Iijima K
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Journal Title
J Matern Fetal Neonatal Med
Volume: -
Issue: 17
Pages: 1820-1822
DOI
Related Report
Peer Reviewed
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[Journal Article] The IgG avidity value for the prediction of congenital cytomegalovirus infection in a prospective cohort study2014
Author(s)
Ebina Y, Minematsu T, Sonoyama A, Morioka I, Inoue N, Tairaku S, Nagamata S, Tanimura K,Morizane M, Deguchi M, Yamada H
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Journal Title
J Perinat Med
Volume: -
Issue: 6
Pages: 755-759
DOI
Related Report
Peer Reviewed
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[Journal Article] Postnatal serum concentrations of endogenous free fatty acids in newborns admitted to the neonatal intensive care unit: effects on unbound bilirubin2014
Author(s)
Koda T, Morioka I, Yokota T, Kurokawa D, Fujita K, Nagasaka M, Matsuo K, Shibata A, Sato I,Kawano S, Yamada H, MD, Nakamura H, Iijima K
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Journal Title
Ann Clin Biochem
Volume: -
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Peer Reviewed
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[Journal Article] Novel treatment strategy for Japanese newborns with high serum unbound bilirubin2013
Author(s)
Yokota T, Morioka I, Kodera T, Morisawa T, Sato I, Kawano S, Koda T, Matsuo K, Fujioka K,Morikawa S, Miwa A, Shibata A, Yokoyama N, Yonetani M, Yamada H, Nakamura H, Iijima K
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Journal Title
Pediatr Int
Volume: 55
Issue: 1
Pages: 54-59
DOI
Related Report
Peer Reviewed
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[Journal Article] Impact of pre-emptive contact precautions for outborn newborns on the incidence of healthcare- associated methicillin-resistant Staphylococcus aureus transmission in a Japanese neonatal intensive care unit2013
Author(s)
Morioka I, Yahata M, Shibata A, Miwa A, Yokota T, Jikimoto T, Nakamura M, Lee M, Yoshida H,Yamada H, Arakawa S, Iijima K
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Journal Title
J Hosp Infect
Volume: 84
Issue: 1
Pages: 66-70
DOI
Related Report
Peer Reviewed
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[Journal Article] Polymorphisms in TLR-2 are associated with congenital cytomegalovirus (CMV) infection, but not with congenital CMV disease2013
Author(s)
Taniguchi R, Koyano S, Suzutani T, Goishi K, Ito Y, Morioka I, Oka A, Nakamura H, Yamada H,Igarashi T, Inoue N
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Journal Title
Int J Infect Dis
Volume: 17
Issue: 12
Pages: 1092-1097
DOI
Related Report
Peer Reviewed
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[Journal Article] Severely high serum unbound bilirubin level after abdominal surgery in a newborn2013
Author(s)
Morioka I, Miwa A, Yokota T, Do Huu CT, Nagasaka M, Koda T, Matsuo K, Morikawa S, Shibata A,Hisamatsu C, Nishio H, Yamada H, Nishijima E, Iijima K
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Journal Title
Pediatr Int
Volume: 55
Issue: 3
Pages: 59-62
DOI
Related Report
Peer Reviewed
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